LOCUS       sequence-360983-        1317 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated cytosolic expression of jGCaMP8m under the synapsin 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1317)
  TITLE     GCaMP8/jGCaMP8 AAV plasmids
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-syn-NES-jGCaMP8m-WPRE
FEATURES             Location/Qualifiers
     source          1..1317
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     351..372
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(478..499)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(739..758)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgggtaacc tgaacgaact ggcattgaag ttggccggtt tggacatcat gcatcatcac
       61 catcatcaca cgcgtcgcaa gaagaccttc aaggaggtgg ccaccgccgt gaagatcatc
      121 ggccgcctga tgggcctgaa gatcaacgtc tatatcaagg ccgacaagca gaagaacggc
      181 atcaaggcga acttccacat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac
      241 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      301 ctgagcgtgg agtccaaact ttcgaaagac cccaacgaga agcgcgatca catggtcctg
      361 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt
      421 accggaggga gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      481 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc
      541 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      601 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      661 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag
      721 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      781 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      841 atcctggggc acaagctgga gtacaacctg ccggaccaac tgactgaaga gcagatcgca
      901 gaatacaaag aggctttctc cctatttgac aaggacgggg atgggacaat aacaaccaag
      961 gagctgggga cggtgatgcg gtctctgggg cagaacccca cagaagcaga gctgcaggac
     1021 atgatcaatg aagtagatgc cgacggtgac ggcacaatcg acttccctga gttcctgaca
     1081 atgatggcaa gaaaaatgaa atacagggac acggaagaag aaattagaga agcgttcggt
     1141 gtgtttgata aggatggcaa tggctacatc agtgcagcag agcttcgcca cgtgatgaca
     1201 aaccttggag agaagttaac agatgaagag gttgatgaaa tgatcaggga agcagacatc
     1261 gatggggatg gtcaggtaaa ctacgaagag tttgtacaaa tgatgacagc gaagtaa
//
