LOCUS       sequence-361172-       12357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express FL variant P229S talin1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12357)
  AUTHORS   Azizi L, Varela L, Turkki P, Mykuliak VV, Korpela S, Ihalainen TO, 
            Church J, Hytonen VP, Goult BT
  TITLE     Talin variant P229S compromises integrin activation and associates 
            with multifaceted clinical symptoms.
  JOURNAL   Hum Mol Genet. 2022 Jul 21. pii: 6647920. doi: 10.1093/hmg/ddac163.
  PUBMED    35861643
REFERENCE   2  (bases 1 to 12357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2022 Jul 21. pii: 6647920. doi: 10.1093/hmg/ddac163."
COMMENT     Sequence Label: Full-length P229S talin1
FEATURES             Location/Qualifiers
     source          1..12357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      644..653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8285..8287,8288..8290,8291..9004)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8285..8287,8288..8290,8291..9001)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8330..8351)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8591..8610)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8938..8959
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9145..9266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9182..9201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9236..9255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9273..9728)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9410..9431)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9622..9641
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9755..9859
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9861..10218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9861..10052
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9884..9904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10069..10204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10253..11047
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10253..11047
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10307..10326)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10917..10936
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11235..11254)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11279..11326
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11655..12243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12144..12163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtattag gcaccccagg ctttacacga attcgcagaa gctgccacca tggttgcgct
      661 ttcgctgaag attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat
      721 ggtgtacgac gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc
      781 caacgacttt gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc
      841 tgggaaagct ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa
      901 gcagagaccc ctgaagatcc ggatgttaga cggaacggtg aagactatca tggtggatga
      961 ctccaagacc gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca
     1021 tgatgagtat tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac
     1081 cctgagaaaa gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa
     1141 gttgcacaca gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg
     1201 agtggaagag catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt
     1261 ggattcccga gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct
     1321 gaatggctcc cattctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca
     1381 gatccagttt ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga
     1441 cttcctgccc aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa
     1501 gaattgtggg cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc
     1561 cctcaagact tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa
     1621 actggtgccc aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac
     1681 caaggaggtg attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa
     1741 gagctttact ctggactttg gagactatca ggatggctac tactcagtac agacgacaga
     1801 aggcgagcag atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa
     1861 aagcaaggac cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt
     1921 gtctcccaaa aagtcaacag tccttcagca gcagtacaac cgagtgggga aagtggagca
     1981 cggctctgtg gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca
     2041 ggtggggagc atgccacctg cccagcagca gatcaccagt ggccagatgc accgaggaca
     2101 catgccacct ctgacttcag cccagcaggc gctcactgga accattaact ccagcatgca
     2161 ggctgtgcag gctgcccagg ccactctgga tgactttgag actctacccc ctcttggcca
     2221 ggatgctgcc tccaaagctt ggcgtaagaa caagatggat gaatcaaagc atgagatcca
     2281 ctcccaggta gatgccatca cagctggcac tgcctctgtg gtaaacctga cagcaggaga
     2341 tcctgcagag acagactata cagcagtggg ctgtgcagtc accaccatct cttccaacct
     2401 gacggagatg tcccgtgggg tgaagctatt ggctgccctg ctggaggatg aaggcggcaa
     2461 tggccggccc cttctgcaag cagcaaaggg ccttgcaggg gctgtgtcgg aactgcttcg
     2521 cagtgctcag cccgccagcg ctgagcctcg tcagaacctg ctgcaggcag ccgggaacgt
     2581 gggccaggcc agtggggagc tgttgcagca aattggggaa agtgacactg acccccactt
     2641 ccaggatgtt ctaatgcagc tagcaaacgc agtggcaagt gctgcagccg ccctggtcct
     2701 caaggccaag agtgtggccc agcgcacaga ggattccggg cttcagaccc aagtgattgc
     2761 tgcagctacg caatgtgctc tgtccacttc ccagctggtg gcctgtacca aggtggtagc
     2821 acctacaatc agctcacctg tgtgccaaga acagctagtc gaggctgggc gactggtggc
     2881 caaagctgtg gagggctgtg tgtctgcctc gcaagcagct acagaagatg gacagctgct
     2941 tcgaggggta ggagcagcag ccacggctgt cacccaggcc ctcaatgagc tgctgcagca
     3001 cgtgaaggcc cacgccaccg gagctgggcc tgccggtcgg tatgaccaag ccactgatac
     3061 catcctcacc gtcactgaaa acatcttcag ctccatgggt gatgctgggg agatggtgcg
     3121 ccaggcccgc atcctggccc aagccacctc agacctggtc aatgctatca aggccgatgc
     3181 tgagggggag agtgatctgg agaactctag aaagctcctg agtgctgcca agatcctcgc
     3241 tgatgccacc gccaagatgg tggaggcggc caagggagca gccgcccacc ctgacagtga
     3301 ggaacagcag cagcgactgc gtgaagcagc tgaggggctt cgcatggcca ccaatgcagc
     3361 tgcgcagaac gccatcaaga agaagttggt gcagcgcctg gagcatgcag ccaagcaagc
     3421 tgcagcctct gcaacacaga ccattgctgc agcccaacat gcagcctctg cccccaaggc
     3481 ctctgcgggc ccccagcccc tactggtgca gagctgtaag gccgtggcag agcagattcc
     3541 gttgctggtg cagggtgtcc gaggaagtca agctcagcct gacagcccta gtgctcagct
     3601 cgccctcatt gctgccagcc agagcttcct gcagccaggt gggaagatgg tggcagcggc
     3661 aaaagcctca gtgccaacaa ttcaggacca ggcttcagcg atgcagctga gtcagtgcgc
     3721 aaagaaccta ggcactgccc tggccgagct gcgcactgct gctcagaagg ctcaggaggc
     3781 atgtggacct ttggagatgg attctgcact gagtgttgta caaaatctag agaaagacct
     3841 acaggaaata aaagcagcag ctcgagatgg caagcttaag cccttacctg gggaaacaat
     3901 ggagaagtgt acccaagatc ttggcaacag caccaaagcg gtgagctctg ccatcgccaa
     3961 gctgctgggg gagattgccc agggcaacga gaactatgca ggtattgcag ctcgggatgt
     4021 agctggtgga ctaaggtcac tagcccaggc tgcgcggggt gtggctgcgc tgacatcaga
     4081 tcctgcagtg caggccattg tgcttgacac agccagcgac gttctggaca aggccagcag
     4141 cctcattgaa gaggcaaaaa aagcatctgg acacccaggg gacccagaaa gccagcaaag
     4201 gcttgctcag gtagctaaag cagtgaccca agccctgaac cgctgtgtca gctgcctgcc
     4261 tggccaaaga gacgtggata atgccctacg agccgttgga gatgccagca agcgactcct
     4321 gagtgacttg cttcctccaa gcacggggac atttcaagaa gcacagagcc gattgaatga
     4381 agctgcagct gggttaaatc aggccgccac agaactggtg caagcctccc gaggaactcc
     4441 tcaggacctg gctcgggcct ccggccgatt tggacaggac ttcagcacct tcctggaagc
     4501 tggtgtggag atggccggac aggccccgag ccaggaggac cgagcccagg tggtgtccaa
     4561 cctgaagggc atatccatgt cttcaagcaa acttctcctt gccgctaagg ccttgtccac
     4621 agaccctgct tctcccaacc tcaagagtca gctggctgcg gctgcccggg cagtgacgga
     4681 cagcatcaac cagctcatca ccatgtgcac ccagcaggca cctggccaga aggagtgtga
     4741 caatgcactt cggcagctag agacagtccg agaactcctg gagaatccag tccagcccat
     4801 caacgacatg tcctacttcg gttgcttgga cagtgtcatg gagaactcca aggtcctagg
     4861 tgaggccatg actggcatct cccaaaatgc caagaatgga aatctgccgg agtttggaga
     4921 tgccattgcc acagcctcca aggctctctg tggcttcacg gaggcagccg cacaggcagc
     4981 atatctagtt ggtgtctctg accccaacag ccaagctgga cagcaaggac tggtggaacc
     5041 cacacagttt gcccgtgcaa accaggcaat tcagatggcc tgtcagagtc tgggggagcc
     5101 tggctgtacc caggcccagg tgttatctgc agccactatt gtagccaaac acacatctgc
     5161 attgtgtaac agctgtcgcc tggcttccgc taggactgcc aatcccactg ccaagcgcca
     5221 gtttgtacag tcagccaagg aggtggccaa cagtacagcc aatcttgtca agaccatcaa
     5281 ggcactagat ggggacttca cagaagagaa ccgtgcccag tgccgagcag ccacagcccc
     5341 tctgctggaa gctgtggata acctgagtgc ctttgcctcc aaccctgagt tctccagcgt
     5401 ccctgcccag atcagccctg agggcagagc agccatggag cccattgtaa tctctgctaa
     5461 gacaatgttg gagagtgctg gagggctcat ccagacagct cgggcgttag cagtcaatcc
     5521 ccgagacccc ccacgttggt ctgtgctagc tggccactct cggactgtct cagattccat
     5581 caagaaactt attacaagca tgagggacaa agccccaggg cagctggagt gtgagacagc
     5641 cattgcggct ctgaacagct gcttgcggga cctagatcag gcttcgcttg ccgctgtcag
     5701 ccagcagctt gctccccgtg aaggaatctc tcaagaggct ttgcacaccc agatgctcac
     5761 tgcagtgcaa gaaatttctc atctcattga gccgctggcc agcgctgctc gggctgaagc
     5821 ctcccagctg ggacacaagg tgtcccaaat ggcccagtac tttgaaccac tcaccctggc
     5881 tgcagtgggt gctgcgtcta agaccctgag ccacccgcag cagatggcac ttctggacca
     5941 gactaaaacg ttggcagagt ctgccttgca gttgctatat actgccaagg aggctggtgg
     6001 taaccccaag caagcagcac acacccagga agccctggag gaggctgtgc agatgatgac
     6061 agaggccgta gaagacctga cgacgaccct caacgaagca gccagtgctg caggagtcgt
     6121 tggcggcatg gtggactcta tcactcaggc catcaaccag ctagatgaag gacctatggg
     6181 tgacccagaa ggctcattcg tagattacca gacaaccatg gtgaggacag ccaaggccat
     6241 tgctgtcact gttcaggaga tggtaaccaa gtcaaacacc agccctgaag agctaggccc
     6301 tcttgccaac cagctgacca gtgactatgg ccgactggcc tcacaagcca agcctgcagc
     6361 tgtggctgct gaaaatgaag agataggcgc tcacatcaaa caccgagtac aggagctggg
     6421 ccatggctgc tctgctctgg tcaccaaggc aggtgccttg cagtgtagcc ccagtgatgt
     6481 ctacaccaag aaggagctca tagagtgtgc ccgcagagtg tcagagaagg tctcccatgt
     6541 tctggctgca ctccaggctg ggaatcgtgg tacccaggcc tgcattacag cagccagtgc
     6601 tgtgtctggt atcattgctg acctcgacac caccatcatg ttcgctactg ctggcacact
     6661 taaccgtgag ggtgctgaaa cttttgctga ccaccgggag ggtatcttaa agacagcaaa
     6721 ggttcttgtg gaggacacca aggtcctagt gcagaatgca gctgggagcc aggagaagtt
     6781 ggcacaagcc gcccagtcct ccgtggccac cattacccgc ctcgctgatg tggtcaagct
     6841 cggtgcagcc agcctaggag ccgaagaccc tgaaactcag gtggtgctga tcaatgcagt
     6901 aaaggacgta gccaaggccc tgggtgacct catcagcgct acgaaggctg cagcgggcaa
     6961 agttggggat gaccctgcag tgtggcagct caagaactct gccaaggtga tggtgaccaa
     7021 tgtgacatca ttgctcaaga cagtgaaggc tgtggaagat gaggccacca aaggcacacg
     7081 ggccctagag gcaaccacag agcacatacg tcaggaactg gcggtcttct gttccccaga
     7141 gccacctgcc aagacctcta cccctgaaga cttcatccga atgaccaagg gtattactat
     7201 ggcaacagcc aaagccgttg ctgctggcaa ttcctgtcga caggaagatg tcattgccac
     7261 agccaatctg agccgacggg ctattgcgga catgcttcgg gcttgcaagg aagcagcttt
     7321 ccacccagaa gtggcgcctg atgtacggct ccgagccctg cactatggcc gggagtgtgc
     7381 caatggttac ctggaactgc tggaccacgt gctgctgacc cttcagaagc caaacccaga
     7441 cctgaagcag cagctaactg gacactcaaa gcgggttgct ggctctgtga ctgagctcat
     7501 ccaggctgct gaagccatga aaggaacaga gtgggtggac ccagaggacc ctactgtcat
     7561 tgctgagaat gagctcttgg gagccgcagc cgccatcgag gctgcagcca agaagctgga
     7621 gcagctgaag cccagggcca aacccaagga ggcggatgag tccttgaact ttgaggaaca
     7681 aatcctagaa gctgccaagt ccatcgctgc agccaccagt gcactggtaa aggctgcgtc
     7741 agcagcccag agggagctgg tggctcaagg aaaggtgggc gccattccag ccaatgcact
     7801 ggatgatggg cagtggtcgc agggcctcat ttccgctgcc cgtatggtgg ctgcggccac
     7861 caacaatctg tgtgaagcag ctaacgcagc tgtccagggc catgctagcc aggagaaact
     7921 catttcctca gccaagcaag tagctgcctc cacagctcag ctcctggtag cttgcaaggt
     7981 caaagcagat caggactctg aggcaatgaa acggctccag gctgctggca atgcagtgaa
     8041 gagggcctca gataacctgg taaaggcggc ccagaaagct gcagccttcg aagaccagga
     8101 gaatgagacg gtggtggtga aggagaaaat ggttgggggc attgcccaga ttatcgcagc
     8161 acaggaagag atgcttcgga aggaacgaga gctggaagag gctcggaaaa agctcgccca
     8221 gatccggcag cagcagtaca agttcttgcc ttcagagctt cgagacgagc acggaggatc
     8281 cggaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     8341 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     8401 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     8461 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     8521 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     8581 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     8641 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     8701 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     8761 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     8821 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     8881 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     8941 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     9001 gtaaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
     9061 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     9121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     9181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9241 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     9301 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     9361 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     9421 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     9481 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     9541 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     9601 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     9661 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     9721 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     9781 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9841 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     9901 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9961 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10021 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    10081 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10141 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    10201 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
    10261 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
    10321 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
    10381 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
    10441 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
    10501 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
    10561 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
    10621 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
    10681 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
    10741 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
    10801 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
    10861 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
    10921 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
    10981 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
    11041 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
    11101 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    11161 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
    11221 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
    11281 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
    11341 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
    11401 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
    11461 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
    11521 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    11581 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    11641 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    11701 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    11761 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    11821 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    11881 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11941 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    12001 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    12061 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    12121 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    12181 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    12241 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    12301 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
