LOCUS       sequence-36126-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  TITLE     McCabe Lab Plasmids
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAd/CMV/PBX1-IRES-nEBFP2
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gttaagctat caacaagttt gtacaaaaaa gcaggcttcg
      121 ccaccatgga cgagcagccc aggctgatgc attcccatgc tggggtcggg atggccggac
      181 accccggcct gtcccagcac ttgcaggatg gggccggagg gaccgagggg gagggcggga
      241 ggaagcagga cattggagac attttacagc aaattatgac catcacagac cagagtttgg
      301 atgaggcgca ggccagaaaa catgctttaa actgccacag aatgaagcct gccttgttta
      361 atgtgttgtg tgaaatcaaa gaaaaaacag ttttgagtat ccgaggagcc caggaggagg
      421 aacccacaga cccccagctg atgcggctgg acaacatgct gttagcggaa ggcgtggcgg
      481 ggcctgagaa gggcggaggg tcggcggcag cggcggcagc ggcggcggct tctggagggg
      541 caggttcaga caactcagtg gagcattcag attacagagc caaactctca cagatcagac
      601 aaatctacca tacggagctg gagaaatacg agcaggcctg caacgagttc accacccacg
      661 tgatgaatct cctgcgagag caaagccgga ccaggcccat ctccccaaa
//
