LOCUS       sequence-361389-        8743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of gRNA scaffold, contains intact LTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Iaffaldano BJ, Marino MP, Reiser J
  TITLE     CRISPR library screening to develop HEK293-derived cell lines with 
            improved lentiviral vector titers.
  JOURNAL   Front Genome Ed. 2023 Jul 13;5:1218328. doi: 
            10.3389/fgeed.2023.1218328. eCollection 2023.
  PUBMED    37520398
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Genome Ed."; date: "2023-07-13"; volume: "5:1218328. doi"; pages: " 
            10.3389/fgeed.2023.1218328. eCollection 2023"
COMMENT     Sequence Label: pxpr_050-wt-ltr
FEATURES             Location/Qualifiers
     source          1..8743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..186
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    285..873
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(338..358)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(756..767)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             945..1578
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1656..1777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1693..1712)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1747..1766
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1817..1952
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1972..1991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1973..1991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2001..2018)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2001..2017)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2010..2032)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2159..2614
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2246..2265)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2456..2477
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2640..2744
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2745..2813,2814..3605)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2963..2982)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3776..4364
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4265..4284
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4518..4535
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4652..4673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4688..4693,4694..4711,4712..4718)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4726..4742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4731..4753
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4750..4766
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4750..4766
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4785..4805
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4787..4805
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4833..5059
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5060..5240
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5287..5412
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5905..6138
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6323..6367
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6545..6793
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6545..6565
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6716..6735
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    6967..7084
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7222..8400
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7453..8391
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8348..8368
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(8413..8743,1..269)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8413..8432)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct
       61 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
      121 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
      181 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
      241 ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt
      301 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
      361 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
      421 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
      481 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
      541 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
      601 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
      661 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
      721 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
      781 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
      841 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
      901 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
      961 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     1021 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     1081 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     1141 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     1201 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggagta
     1261 cttcaagaac tgctgacatc gagcttgcta caagggactt tccgctgggg actttccagg
     1321 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatgctgca tataagcagc
     1381 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     1441 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     1501 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     1561 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     1621 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     1681 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1741 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     1801 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     1861 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     1921 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     1981 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2041 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2101 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     2161 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2221 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2281 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2341 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2401 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2461 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2521 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2581 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     2641 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2701 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2761 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2821 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2881 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2941 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3001 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3061 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3121 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3181 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3241 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     3301 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3361 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3421 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3481 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3541 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3601 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3661 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3721 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3781 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3841 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3901 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3961 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4021 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4081 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4141 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4201 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4261 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4321 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4381 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4441 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4501 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4561 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4621 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     4681 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     4741 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     4801 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     4861 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     4921 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     4981 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     5041 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     5101 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5161 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5221 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     5281 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     5341 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     5401 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     5461 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     5521 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     5581 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     5641 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     5701 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     5761 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     5821 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     5881 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     5941 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     6001 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     6061 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     6121 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     6181 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     6241 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     6301 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     6361 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     6421 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     6481 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     6541 cagagagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     6601 agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     6661 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     6721 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     6781 aggacgaaac accggagacg accggttttt tgcgtctctg tttcagagct acagcagaaa
     6841 tgctgtagca agttgaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     6901 ggtgcttttt tgaattcaag cttggcgtaa ctagatcttg agacaaatgg cagtattcat
     6961 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     7021 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     7081 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctca gggggcccgg
     7141 gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     7201 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     7261 acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg
     7321 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     7381 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     7441 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     7501 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
     7561 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     7621 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     7681 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     7741 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     7801 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     7861 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     7921 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     7981 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     8041 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     8101 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     8161 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     8221 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     8281 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     8341 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     8401 cgtacggcca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8461 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8521 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8581 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     8641 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     8701 gagttgagcg gttcccggct ggccgcgcag caacagatgg aag
//
