LOCUS       sequence-36143-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pAd:TRE-gateway
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..364)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(161..180)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 acatatccag tcactatggt cgacctgcag actggctgtg tataagggag cctgacattt
       61 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
      121 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
      181 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca
      241 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac
      301 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
      361 gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg
      421 acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct
      481 tcattctgca tggttgtgct taccagaccg gagatattga catcatatat gccttgagca
      541 actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca tacctctttt
      601 tgacatactt c
//
