LOCUS       sequence-36145-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  TITLE     3I4W
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PDZ3-pET28a
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             353..370
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(423..441)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      437..484
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      521..949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(608..627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     818..839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggtgagg aagacattcc
       61 gcgtgaaccg cgtcgtatcg tgatccatcg tggctccacc ggcctgggct tcaacatcgt
      121 gggcggcgag gatggtgaag gcatcttcat ctccttcatc cttgctgggg gtccagccga
      181 cctcagtggg gagctacgga agggggacca gatcctgtcg gtcaatggtg ttgacctccg
      241 caatgccagt cacgaacagg ctgccattgc cctgaagaat gcgggtcaga cggtcacgat
      301 catcgctcag tataaaccag aagagtatag tcgattcgag gcctgactcg agcaccacca
      361 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      421 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      481 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      541 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      601 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      661 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      721 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      781 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
      841 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      901 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      961 ttaacgctta ca
//
