LOCUS       sequence-361706-        4537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked 
            by Gateway recombination sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero 
            C, Mueller F, Lemaire P
  TITLE     A multicassette Gateway vector set for high throughput and 
            comparative analyses in ciona and vertebrate embryos.
  JOURNAL   PLoS One. 2007. 2(9):e916.
  PUBMED    17878951
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2007. 2(9):e916."
COMMENT     Sequence Label: pENT-L3-pFOGl-L5
FEATURES             Location/Qualifiers
     source          1..4537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    576..671
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2659..2754)
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2778..2797)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2779..2797)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2802..2818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2802..2818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2920..2939)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2931..3740
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2998..3017)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3887..4475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4376..4395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccct ctagaaaata atgattttat tttgactgat
      601 agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta
      661 taataaagta ggctgcaact attgtaacac cacacggggg cgcagtagag ccagaatctt
      721 agtaaatgcg gtttgattgt aaagttttta acaatctctc gctttgtata ttctagaatg
      781 gggaaagatg ggacccttaa gcactcattg cccaatattt ccaaacccaa aatctacgat
      841 ggttttgtgt ggtaccacga attagtacta tcatatacac ttcaaagaaa tatattaaac
      901 catacgagaa gtaatttatc tattcgcaca acaggtgtaa ctttggaaat tcgaacacag
      961 gcgagctagt tttatgacac gtaaacttta tatatttctt ttctaattta aggaatattg
     1021 accactgccc atttttcttg ttttaatatc ccagttttta aaatggcctg aaatatttca
     1081 gcttatttta ttaaattacg atactggatt gatgattcaa attaacaaac taccaaaaaa
     1141 tgttgacagt tttaatatga tttaccgagc accacgtgta acagcatcat acaaatttaa
     1201 ttgattttgt attacttact ttataattta aacatacggt atttagtaaa caaagaacat
     1261 tcaaagaatt agaaaaccgt attcccatga ctttcataga ccgttgttga ttgtttaaaa
     1321 catgatcata atatgtaggt attatatgtg ccaaaggtgt cccgttttcc cccacccata
     1381 ctatatatat tataaaaaaa aatggcgtta taaaaaaact ataccctgca tgtatttata
     1441 gatttgttaa acgtatgatc tcgtgttatg atctatccgt atagtggacc aggaatcttt
     1501 gggttgtgca tatttaactg agaaatcgac aagcaattaa acgcggcagt atacgacatg
     1561 aacaatataa ttggcgcatt ataattagat tgtataccac gcactaccta aaaagttaag
     1621 ctataactga cgtcaggtat tatttattgg tatccggcaa cactaataaa tgaaattttg
     1681 tcaaaatgta agccctcgta tctatttaaa ttttgacgct aaataatcca tttatgcagt
     1741 ataataccaa acatacaaaa tgtttcagtt tgtatcactt tattatgcat cacgtttttg
     1801 ggataaatac acatcaggat ttaacaaaat atgttaaata caatatggcg gtattgcttt
     1861 cacttaaaag tgttaacaat taagatggga aaaataaaac agtaaatata ttctttaaat
     1921 cgaacttttg ttgtaaatct tctattttaa taatttattc atgtttaaaa cgatgtttgt
     1981 ttcggcgtca tgctgctcgt taggtataac ttcactcggg acattttagt atcgagttgc
     2041 tcaaaattgt tagggttgtc aactgaatag cgatgttttc gattgagtta atatttctat
     2101 acatgatggc atcagctagt aagatagaaa acaaccttgt tattactcgt gctgtaataa
     2161 tataattcat gaaaaacaaa catggtctta tcaaatctat tccgaagaca aacatacggc
     2221 tgatagaatt cagtcagcgc ggtttgggcg acatcactca gagctctagc tcttatcttg
     2281 cttatcgagc aagcgataaa acaagcagta acgagaaaac aagagaaaga aggctagctt
     2341 cctggagaag accaagataa agtatctcaa aattcaggaa acggtccaag accgaagctc
     2401 caaagctctt gtgttcagtt aaactctgat agtgaataag cttcgtgtat tgtaccgacc
     2461 cattgtcaat catgcaaact tgatattata ttgacaagag aagaaggcag tttaaattaa
     2521 aactctaaag tagagagaca ttaatctcag ctgacaaggc aggtggtcac agtaagttca
     2581 tttaaatagt tggccaacaa cagcttttcc aagaaagtat ttttgtttca ggtctataca
     2641 aaaataacac acataaccca acttttgtat acaaagttgg cattataaga aagcattgct
     2701 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     2761 cagctctaga tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     2821 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa ataatatcat
     2881 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     2941 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     3001 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     3061 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     3121 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     3181 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     3241 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     3301 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     3361 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     3421 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     3481 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     3541 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     3601 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     3661 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     3721 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     3781 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     3841 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3901 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3961 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4021 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4081 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4141 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4201 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4261 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4321 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4381 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4441 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4501 ggttcctggc cttttgctgg ccttttgctc acatgtt
//
