LOCUS       sequence-361782-        3207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: 
            tgcc - MCS - gagc).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM246 - mosTI - unc-119 - MCS
FEATURES             Location/Qualifiers
     source          1..3207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(11..802,803..871))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASQQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     634..653
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(872..976)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1075..1097
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1089..1106
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1090..1106
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1730..1763)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2201..2217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2201..2217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2214..2236)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2389..2977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2469..2488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(3094..3118)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
       61 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
      121 catctggccc cagtgctgca atgataccgc gagagccacg ctcaccggct ccagatttat
      181 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
      241 cctccatcca gtctattaat tgttgctggg aagctagagt aagtagttcg ccagttaata
      301 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
      361 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
      421 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
      481 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
      541 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
      601 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
      661 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
      721 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
      781 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
      841 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
      901 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
      961 aaataggggt tccgcgcaca tttccccgaa aagtgccaga tacctgaaac aaaacccatc
     1021 gtacggccaa ggaagtctcc aataactgtg atccaccaca agcgccaggg ttttcccagt
     1081 cacgacgttg taaaacgacg gccagtcatg cataatccgc acgcatctgg aataaggaag
     1141 tgccattccg cctgacctaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     1201 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     1261 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     1321 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     1381 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     1441 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     1501 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     1561 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatct
     1621 tcattgttga gtttatcttg ttgatttttg aataaattat caactcttta ctttttaatg
     1681 ggttatgaaa taaataaaca ttgaaaactg ataaacaacg ttcatctcta taacttcgta
     1741 tagcatacat tatacgaagt tattgcctga gaccagctag caagcttgca tgcctgcagg
     1801 tcgactctag aggatccccg ggtaccgagc tcaggtctct gagctcagga aacggagaat
     1861 ctgtgcggca atgtcttatc atttgaaacg cgccgtatcg atgctgacat catgcggctc
     1921 ccgtgttagt cgagaagatg ggtacgggcc tttattcatc caacactcta cggcttcttc
     1981 aagagctagt agggcaccct gtagttggaa agggattatt tcgtatggcg agcccatacc
     2041 gtctttcttg cggaagctta acacgatagg aagttggata gtttcgaacg atggttccat
     2101 acggaacgct gtctcaagga tgagtctgac ggagtgtaac tcgaaggcta ggtggaggct
     2161 cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt
     2221 gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca atctccaaga
     2281 cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa
     2341 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2401 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2461 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2521 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2581 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2641 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2701 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2761 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2821 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2881 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2941 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3001 ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta aatgggtagg
     3061 gggcttcaaa tcgtcctcgt gataccaatt cggagcctgc ttttttgtac aaacttgttg
     3121 ataatggcaa ttcaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3181 caatctaaag tatatatgag taaactt
//
