LOCUS       sequence-361957-        3538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of lambda-CI fused via three alanines to the
            MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Pandey S, Gravel CM, Stockert OM, Wang CD, Hegner CL, LeBlanc H, 
            Berry KE
  TITLE     Genetic identification of the functional surface for RNA binding by 
            Escherichia coli ProQ.
  JOURNAL   Nucleic Acids Res. 2020 May 7;48(8):4507-4520. doi: 
            10.1093/nar/gkaa144.
  PUBMED    32170306
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-05-7- 7"; volume: "48"; issue: "8";
            pages: "4507-4520"
COMMENT     Sequence Label: pCW17 - pAdapter
FEATURES             Location/Qualifiers
     source          1..3538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1620..2330
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             complement(join(3098..3538,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagctaa ttctcactca ttaggcaccc caggcctcga
     1561 gacgatagct agctcagtcc taggtatagt gctagcgcat gccacacagg aaacagcgta
     1621 tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag
     1681 caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga
     1741 tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt
     1801 ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa
     1861 tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg
     1921 agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa
     1981 cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg
     2041 cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct
     2101 ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct
     2161 gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc
     2221 aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt
     2281 ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gcggccgcag
     2341 aattcccggg gattcacccg ggtatggctt ctaactttac tcagttcgtt ctcgtcgaca
     2401 atggcggaac tggcgacgtg actgtcgccc caagcaactt cgctaacggg atcgctgaat
     2461 ggatcagctc taactcgcgt tcacaggctt acaaagtaac ctgtagcgtt cgtcagagct
     2521 ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc taaaggcgcc tggcgttcgt
     2581 acttaaatat ggaactaacc attccaattt tcgccacgaa ttccgactgc gagcttattg
     2641 ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcagca atcgcagcaa
     2701 actccggcat ctactaagga tccttacctt acagatctgc atcgcaggat gctgctggct
     2761 accctgtgga acacctacat ctgtattaac gaagcgctaa ccgtttttat caggctctgg
     2821 gaggcagaat aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac
     2881 tggcctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     2941 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg
     3001 aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcaac
     3061 caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
     3121 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     3181 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     3241 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     3301 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     3361 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     3421 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     3481 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacg
//
