LOCUS       sequence-361984-       10509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector; Tet-on advanced system driving the expression of 
            tagged HNF1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Beucher A, Miguel-Escalada I, Balboa D, De Vas MG, Maestro MA, 
            Garcia-Hurtado J, Bernal A, Gonzalez-Franco R, Vargiu P, Heyn H, 
            Ravassard P, Ortega S, Ferrer J
  TITLE     The HASTER lncRNA promoter is a cis-acting transcriptional 
            stabilizer of HNF1A.
  JOURNAL   Nat Cell Biol. 2022 Oct 6. pii: 10.1038/s41556-022-00996-8. doi: 
            10.1038/s41556-022-00996-8.
  PUBMED    36202974
REFERENCE   2  (bases 1 to 10509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2022 Oct 6. pii: 10.1038/s41556-022-00996-8. doi: 
            10.1038/s41556-022-00996-8."
COMMENT     Sequence Label: pLenti-CMVtight-HNF1A-FLAG-Hygro
FEATURES             Location/Qualifiers
     source          1..10509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     125..144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     378..395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    512..533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(548..553,554..571,572..578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    586..602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     591..613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     610..626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     610..626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     645..665
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        647..665
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        693..919
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             920..1100
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1147..1272
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1765..1998
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2183..2227
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2494..2611
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2633..2947
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2641..2659
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2677..2695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2712..2730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2748..2766
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2784..2802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2819..2837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2855..2873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2920..2944
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3025..3049
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4545..4554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4980..5003
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5016..5060
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     protein_bind    complement(5105..5129)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5160..5748
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5213..5233)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5631..5642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5804..6303
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5839..5862)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6236..6255
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6321..7346
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6321..7346
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7720..7953
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8025..8146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8062..8081)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8116..8135
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8186..8321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8341..8360)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8342..8360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8370..8387)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8370..8386)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8379..8401)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8528..8983
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8615..8634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8825..8846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9009..9113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9114..9182,9183..9974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9332..9351)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10145..10509,1..224)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
       61 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      301 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      361 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      421 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
      481 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      541 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      601 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
      661 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
      721 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
      781 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
      841 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
      901 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
      961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     1021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     1081 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     1141 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     1201 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     1261 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     1321 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     1381 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     1441 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     1501 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     1561 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     1621 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1681 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1741 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1801 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1861 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1921 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1981 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     2041 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     2101 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     2161 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     2221 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2281 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2341 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2401 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2461 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     2521 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2581 acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgagtttac
     2641 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
     2701 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2761 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
     2821 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     2881 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     2941 gatcgcctgg agaattcgag ctcggtaccc ggggatccac cggttcgtcg actagtccag
     3001 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc tttaaaggaa
     3061 ccaattcagt cgacatggtt tctaaactga gccagctgca gacggagctc ctggcggccc
     3121 tgctggagtc agggctgagc aaagaggcac tgatccaggc actgggtgag ccggggccct
     3181 acctcctggc tggagaaggc cccctggaca agggggagtc ctgcggcggc ggtcgagggg
     3241 agctggctga gctgcccaat gggctggggg agactcgggg ctccgaggac gagacggacg
     3301 acgatgggga agacttcacg ccacccatcc tcaaagagct ggagaacctc agccctgagg
     3361 aggcggccca ccagaaagcc gtggtggaga cccttctgca ggaggacccg tggcgtgtgg
     3421 cgaagatggt caagtcctac ctgcagcagc acaacatccc acagcgggag gtggtcgata
     3481 ccactggcct caaccagtcc cacctgtccc aacacctcaa caagggcact cccatgaaga
     3541 cgcagaagcg ggccgccctg tacacctggt acgtccgcaa gcagcgagag gtggcgcagc
     3601 agttcaccca tgcagggcag ggagggctga ttgaagagcc cacaggtgat gagctaccaa
     3661 ccaagaaggg gcggaggaac cgtttcaagt ggggcccagc atcccagcag atcctgttcc
     3721 aggcctatga gaggcagaag aaccctagca aggaggagcg agagacgcta gtggaggagt
     3781 gcaatagggc ggaatgcatc cagagagggg tgtccccatc acaggcacag gggctgggct
     3841 ccaacctcgt cacggaggtg cgtgtctaca actggtttgc caaccggcgc aaagaagaag
     3901 ccttccggca caagctggcc atggacacgt acagcgggcc ccccccaggg ccaggcccgg
     3961 gacctgcgct gcccgctcac agctcccctg gcctgcctcc acctgccctc tcccccagta
     4021 aggtccacgg tgtgcgctat ggacagcctg cgaccagtga gactgcagaa gtaccctcaa
     4081 gcagcggcgg tcccttagtg acagtgtcta cacccctcca ccaagtgtcc cccacgggcc
     4141 tggagcccag ccacagcctg ctgagtacag aagccaagct ggtctcagca gctgggggcc
     4201 ccctcccccc tgtcagcacc ctgacagcac tgcacagctt ggagcagaca tccccaggcc
     4261 tcaaccagca gccccagaac ctcatcatgg cctcacttcc tggggtcatg accatcgggc
     4321 ctggtgagcc tgcctccctg ggtcctacgt tcaccaacac aggtgcctcc accctggtca
     4381 tcggcctggc ctccacgcag gcacagagtg tgccggtcat caacagcatg ggcagcagcc
     4441 tgaccaccct gcagcccgtc cagttctccc agccgctgca cccctcctac cagcagccgc
     4501 tcatgccacc tgtgcagagc catgtgaccc agagcccctt catggccacc atggctcagc
     4561 tgcagagccc ccacgccctc tacagccaca agcccgaggt ggcccagtac acccacacgg
     4621 gcctgctccc gcagactatg ctcatcaccg acaccaccaa cctgagcgcc ctggccagcc
     4681 tcacgcccac caagcaggtc ttcacctcag acactgaggc ctccagtgag tccgggcttc
     4741 acacgccggc atctcaggcc accaccctcc acgtccccag ccaggaccct gccggcatcc
     4801 agcacctgca gccggcccac cggctcagcg ccagccccac agtgtcctcc agcagcctgg
     4861 tgctgtacca gagctcagac tccagcaatg gccagagcca cctgctgcca tccaaccaca
     4921 gcgtcatcga gaccttcatc tccacccaga tggcctcttc ctcccaggaa ttcactagtg
     4981 actacaaaga cgatgacgat aaaggtgatg ggtccggcct gaacgacatc ttcgaggctc
     5041 agaaaatcga atggcacgaa ggcgcgccga gctcgaggta ggcggccgca ctcgagatat
     5101 ctagacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca
     5161 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5221 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5281 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5341 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5401 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5461 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5521 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5581 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5641 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5701 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctgc
     5761 agatatccgg actagtgatc tctcgaggtt aacgaattct accgggtagg ggaggcgctt
     5821 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     5881 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     5941 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     6001 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     6061 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     6121 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     6181 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     6241 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     6301 tcgacctgca tcccgccacc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     6361 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     6421 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     6481 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     6541 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     6601 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     6661 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     6721 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     6781 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     6841 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     6901 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     6961 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     7021 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     7081 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     7141 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     7201 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     7261 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     7321 gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc tgatttcgag
     7381 aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga acggccttac
     7441 gcttggtggc acagttctcg tccacagttc gctaagctcg ctcggctggg tcgcgggagg
     7501 gccggtcgca gtgattcagg cccttctgga ttgtgttggt ccccagggca cgattgtcat
     7561 gcccacgcac tcgggtgatc tgactgatcc cgcagattgg agatcgccgc ccgtgcctgc
     7621 cgattgggtg cagatccgtc gagggcccgg tacctttaag accaatgact tacaaggcag
     7681 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc
     7741 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7801 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7861 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7921 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7981 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     8041 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8101 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     8161 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8221 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8281 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     8341 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8401 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8461 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8521 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8581 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8641 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8701 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8761 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8821 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8881 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8941 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     9001 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9061 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9121 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9181 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9241 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9301 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9361 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9421 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9481 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9541 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9601 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9661 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9721 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9781 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9841 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9901 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9961 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10021 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10081 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10141 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10201 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10261 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10321 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10381 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10441 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10501 cgacctaca
//
