LOCUS       sequence-362154-        9554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human SOX2 under the human GFAP ABC1D promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Niu W, Zang T, Zou Y, Fang S, Smith DK, Bachoo R, Zhang CL
  TITLE     In vivo reprogramming of astrocytes to neuroblasts in the adult 
            brain.
  JOURNAL   Nat Cell Biol. 2013 Oct;15(10):1164-75. doi: 10.1038/ncb2843. Epub 
            2013 Sep 22.
  PUBMED    24056302
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2843"; 
            journalName: "Nat Cell Biol"; date: "2013-10"; volume: "15"; issue: 
            "10"; pages: "1164-75"
COMMENT     Sequence Label: LV-hGFAP-SOX2
FEATURES             Location/Qualifiers
     source          1..9554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1648..2236
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1701..1721)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2119..2130)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2755..2935
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2961..2978)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2967..3191
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3237..3665
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3324..3343)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3534..3555
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3674..3694)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3679..4008
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3859..3994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3921..3940
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4056..4103
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4122..4496
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4626..4747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4663..4682)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4717..4736
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4796..4812)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4796..4812)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4809..4831)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4820..4836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4844..4850,4851..4868,4869..4874))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4889..4910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5027..5044)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5198..5786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5278..5297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5957..6748,6749..6817))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6818..6922)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6997..7016)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7188..7567
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7569..7767
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7722..7742
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7785..7965
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8012..8137
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8630..8863
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9048..9092
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9366..9483
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctctgacaga ggctcggggg cctgagctgg ctctgtgagc tggggaggag gcagacagcc
       61 aggccttgtc tgcaagcaga cctggcagca ttgggctggc cgccccccag ggcctcctct
      121 tcatgcccag tgaatgactc accttggcac agacacaatg ttcggggtgg gcacagtgcc
      181 tgcttcccgc cgcaccccag cccccctcaa atgccttccg agaagcccat tgagcagggg
      241 gcttgcattg caccccagcc tgacagcctg gcatcttggg ataaaagcag cacagccccc
      301 taggggctgc ccttgctgtg tggcgccacc ggcggtggag aacaaggctc tattcagcct
      361 gtgcccagga aaggggatca ggggatgccc aggcatggac agtgggtggc agggggggag
      421 aggagggctg tctgcttccc agaagtccaa ggacacaaat gggtgagggg agagctctcc
      481 ccatagctgg gctgcggccc aaccccaccc cctcaggcta tgccaggggg tgttgccagg
      541 ggcacccggg catcgccagt ctagcccact ccttcataaa gccctcgcat cccaggagcg
      601 agcagagcca gagcaggttg gagaggagac gcatcacctc cgctgctcgc accggtgcca
      661 ccatgtacaa catgatggag acggagctga agccgccggg cccgcagcaa acttcggggg
      721 gcggcggcgg caactccacc gcggcggcgg ccggcggcaa ccagaaaaac agcccggacc
      781 gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg cgggcagcgg cgcaagatgg
      841 cccaggagaa ccccaagatg cacaactcgg agatcagcaa gcgcctgggc gccgagtgga
      901 aacttttgtc ggagacggag aagcggccgt tcatcgacga ggctaagcgg ctgcgagcgc
      961 tgcacatgaa ggagcacccg gattataaat accggccccg gcggaaaacc aagacgctca
     1021 tgaagaagga taagtacacg ctgcccggcg ggctgctggc ccccggcggc aatagcatgg
     1081 cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt gaaccagcgc atggacagtt
     1141 acgcgcacat gaacggctgg agcaacggca gctacagcat gatgcaggac cagctgggct
     1201 acccgcagca cccgggcctc aatgcgcacg gcgcagcgca gatgcagccc atgcaccgct
     1261 acgacgtgag cgccctgcag tacaactcca tgaccagctc gcagacctac atgaacggct
     1321 cgcccaccta cagcatgtcc tactcgcagc agggcacccc tggcatggct cttggctcca
     1381 tgggttcggt ggtcaagtcc gaggccagct ccagcccccc tgtggttacc tcttcctccc
     1441 actccagggc gccctgccag gccggggacc tccgggacat gatcagcatg tatctccccg
     1501 gcgccgaggt gccggaaccc gccgccccca gcagacttca catgtcccag cactaccaga
     1561 gcggcccggt gcccggcacg gccattaacg gcacactgcc cctctcacac atgtgatgta
     1621 caacttcgag ggatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     1681 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1741 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     1801 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     1861 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     1921 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     1981 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2041 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     2101 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2161 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2221 tgggccgcct ccccgcatcg ataccgtcga gacctagaaa aacatggagc aatcacaagt
     2281 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     2341 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     2401 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     2461 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     2521 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     2581 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     2641 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     2701 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     2761 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     2821 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     2881 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     2941 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3001 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3061 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3121 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3181 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     3241 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3301 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3361 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3421 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3481 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3541 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     3601 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     3661 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     3961 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4021 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     4081 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     4141 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     4201 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     4261 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     4321 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     4381 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     4441 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     4501 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     4561 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     4621 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4681 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4741 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     4801 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4861 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     4921 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     4981 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5041 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5101 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5161 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5221 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5281 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5341 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5401 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5461 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5521 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5581 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5641 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5701 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5761 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5821 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5881 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5941 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6001 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6061 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6121 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6181 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6241 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6301 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6361 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6421 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6481 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6541 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6601 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6661 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6721 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6781 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6841 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6901 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     6961 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     7021 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     7081 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     7141 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     7201 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     7261 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     7321 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     7381 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     7441 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     7501 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     7561 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     7621 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     7681 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     7741 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     7801 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7861 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7921 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     7981 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8041 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8101 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8161 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8221 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8281 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8341 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     8401 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8461 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     8521 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     8581 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     8641 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     8701 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     8761 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     8821 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     8881 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     8941 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     9001 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     9061 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     9121 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     9181 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     9241 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     9301 agagagacag agacagatcc attcgattag tgaacggatc tacaaatggc agtattcatc
     9361 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     9421 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     9481 tttcgggttt attacaggga cagcagaaat tcactttgaa ttcaacatat cctggtgtgg
     9541 agtaggggac gctg
//
