LOCUS       sequence-36228-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Agarwala S, Aglyamova GV, Marma AK, Fallon JF, Ragsdale CW
  TITLE     Differential susceptibility of midbrain and spinal cord patterning 
            to floor plate defects in the talpid2 mutant.
  JOURNAL   Dev Biol. 2005 Dec 1. 288(1):206-20.
  PUBMED    16246323
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2005 Dec 1. 288(1):206-20."
COMMENT     Sequence Label: pcDISP1
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(759..777)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(760..777)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(795..811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(795..811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(808..830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    819..835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(843..849,850..866,867..872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    887..908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gctcgagcgg ccgccagtgt gatggatatc tgcagaattc gcccttaagg tctgagagaa
       61 ggcattacac cgcagttttt ttggtagcgg gatctggcca caagtgccct ggggtccaag
      121 gcatcggcac atgcactgga agaagaatgt tgcaaaagcc caactaatgc acattattag
      181 catcatgaaa gtgccgagct gcgtgtacgc caacactgta gaaggcatca tcattgctcc
      241 agctacaaac gttgtcaacg cagccatcgc aatagcagaa cccattcggc ttagagaaaa
      301 aatcactttt ccctctcggt cagggtcggg ggctaagcga taagctactc cgtaatgaac
      361 agcaaagtct acagacaagc cgacagctac agaaatagtg acagattcta gcacatttag
      421 ctcccagcca agaagaacca gggaacctac agtgacaaaa atagttccag cgattgaaac
      481 tattgcataa aggcttataa taatgttcca agttgtaaga agcattacac taaaggcaac
      541 agcaaccgaa agacccatgg caattagagt tccatcagaa agactgtcct gtagatcata
      601 aaattctaaa ttactcacaa accaaccatt gctaaggcct tcaggagctg aactaagctc
      661 acttgaaatc cacgagtcca agggcgaatt ccagcacact ggcggccgtt actagtggat
      721 ccgagctcgg taccaagctt gatgcatagc ttgagtattc tatagtgtca cctaaatagc
      781 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      841 cacaacatac gagccggagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      901 tcacattaat tgcgttgcgc tcactgc
//
