LOCUS       sequence-362369-       11626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for stable integration of Renilla luciferase NMD(+) 
            PTC39 reporter at AAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-A...
FEATURES             Location/Qualifiers
     source          1..11626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    1129..1210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1327..2073)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2092..2602)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     polyA_signal    2622..2670
                     /note="synthetic polyadenylation signal"
     misc_feature    2684..2775
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2724..2743
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2779..3146
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2787..2805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2823..2841
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2859..2877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2895..2913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2931..2949
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2967..2985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3003..3021
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3181..3246
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3265..4194
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="TSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    4222..4246
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5649..5730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5752..6262
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             6284..6883
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6284..6303)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6780..6800
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6890..7114
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    7128..7964
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7998..8017)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7999..8017)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8024..8041)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8024..8040)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8033..8055)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8178..8480
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8359..8378
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             8829..9623
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8829..9623
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8883..8902)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9493..9512
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9873..10664,10665..10733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10496..10515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10857..11445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11346..11365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gctgagtcag tgagcgagga agctcggggc agtgaaaaaa
     1141 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1261 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     1321 ggagatttac ccggggagca tgtcaaggtc aaaatcgtca agagcgtcag caggcagcat
     1381 atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg tctaagtcaa aatcgtcaag
     1441 ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc tccaggccac atatgattag
     1501 ttccaggccg aaaaggaagg caggttcggc tccctgccgg tcgaacagct caattgcttg
     1561 tttcagaagt gggggcatag aatcggtggt aggtgtctct ctttcctctt ttgctacttg
     1621 atgctcctgt tcctccaata cgcagcccag tgtaaagtgg cccacggcgg acagagcgta
     1681 cagtgcgttc tccagggaga agccttgctg acacaggaac gcgagctgat tttccagggt
     1741 ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact ttagccccgt cgcgatgtga
     1801 gaggagagca cagcggtatg acttggcgtt gttccgcaga aagtcttgcc atgactcgcc
     1861 ttccaggggg caggagtggg tatgatgcct gtccagcatc tcgattggca gggcatcgag
     1921 cagggcccgc ttgttcttca cgtgccagta cagggtaggc tgctcaactc ccagcttttg
     1981 agcgagtttc cttgtcgtca ggccttcgat accgactcca ttgagtaatt ccagagcaga
     2041 gtttatgact ttgctcttgt ccagtctaga catggtaatt cgatgatccc cctggggaga
     2101 gaggtcggtg attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca
     2161 gaatgcggaa caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc
     2221 ccgcaccgcc ccttcccggc cgctgctctc ggcgcgccct gctgagcagc cgctattggc
     2281 cacagcccat cgcggtcggc gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt
     2341 actagtgaga cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc
     2401 ttcccgactt aggggcggag caggaagcgt cgccgggggg cccacaaggg tagcggcgaa
     2461 gatccgggtg acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt cggcgccgct
     2521 gcgtttcccg gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg
     2581 gaaaaggcgc aaccccaacc ccgtggaatt atcacctcga gaataaaata tctttatttt
     2641 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     2701 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     2761 ccagaacatt tctctctcga gtttactccc tatcagtgat agagaacgta tgaagagttt
     2821 actccctatc agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg
     2881 tataaggagt ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag
     2941 tgatagagaa cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt
     3001 actccctatc agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa
     3061 aagagtgctg attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt
     3121 tccgtaccac ttcctaccct cgtaaagaat tcgcggccgc aaccgtcaga attaaccatg
     3181 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
     3241 gacaagcttg cggccgcgaa ttcaacttcg aaagtttatg atccagaaca aaggaaacgg
     3301 atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt
     3361 attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac
     3421 gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt
     3481 attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg
     3541 ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag
     3601 atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat
     3661 caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg
     3721 gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa
     3781 atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag
     3841 ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt
     3901 cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt
     3961 gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg
     4021 tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt
     4081 cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
     4141 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaagcgaat
     4201 tcatcgatag atctgatatc aacaagtttg tacaaaaaag caggctccgc ggccgccccc
     4261 ttcaccgtgc atctgactcc tgaggagaag tctgccgtta ctgccctgtg gggcaaggtg
     4321 aacgtggatg aagttggtgg tgaggccctg ggcaggttgg tatcaaggtt acaagacagg
     4381 tttaaggaga ccaatagaaa ctgggcatgt ggagacagag aagactcttg ggtttctgat
     4441 aggcactgac tctctctgcc tattggtcta ttttcccacc cttaggctgc tggtggtcta
     4501 cccttggacc tagaggttct ttgagtcctt tggggatctg tccactcctg atgctgttat
     4561 gggcaaccct aaggtgaagg ctcatggcaa gaaagtgctc ggtgccttta gtgatggcct
     4621 ggctcacctg gacaacctca agggcacctt tgccacactg agtgagctgc actgtgacaa
     4681 gctgcacgtg gatcctgaga acttcagggt gagtctgatg ggcacctcct gggtttcctt
     4741 cccctggcta ttctgctcaa ccttcctatc agaaaaaaag gggaagcgat tctagggagc
     4801 agtctccatg actgtgtgtg gagtgttgac aagagttcgg atattttatt ctctactcag
     4861 aattgctgct ccccctcact ctgttctgtg ttgtcatttc ctctttcttt ggtaagcttt
     4921 taatttccag ttgcatttta ctaaattaat taagctggtt atttacttcc catcctgata
     4981 tcagcttccc ctcctccttt cctcccagtc cttctctctc tcctctctct ttctctaatc
     5041 ctttcctttc cctcagttca tttcttcttc tttgatctac gtttgtttgt ctttttaaat
     5101 attgccttgt aacttgctca gaggacaagg aagatatgtc cctgtttctt ctcatagctc
     5161 tcaagaatag tagcataatt ggcttttatg ccagggtgac aggggaagaa tatattttac
     5221 atataaattc tgtttgacat aggattctta taataatttg tcagtagttt aaggttgcaa
     5281 acaaatgtct ttgtaaataa gcctgcagta tctggtattt ttgctctaca gttatgttga
     5341 tggttcttcc atattcccac agctcctggg caatatgatc gtgattgtgc tgggccacca
     5401 cctgggcaag gatttcaccc ccgctgcaca ggctgccttc cagaaggtgg tggctggagt
     5461 ggccactgcc ctggcccaca agtatcacta aaagggtggg cgcgccgacc cagctttctt
     5521 gttctgcagc atatgtctag aggatcataa tcagccatac cacatttgta gaggttttac
     5581 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5641 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5701 atttcacaaa taaagcattt ttttcactgc cgacgtcagg tggcactttt cggggttggg
     5761 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     5821 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     5881 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     5941 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     6001 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     6061 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     6121 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     6181 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     6241 aatcaccgac ctctctcccc agggggatcc accggagctt accatgaccg agtacaagcc
     6301 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6361 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6421 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6481 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6541 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6601 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6661 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6721 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6781 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6841 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaggtaccc tgtgccttct
     6901 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6961 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7021 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7081 agcaggcatg ctggggatgc ggtgggctct atggggtacc aagctttact agggacagga
     7141 ttggtgacag aaaagcccca tccttaggcc tcctccttcc tagtctcctg atattgggtc
     7201 taacccccac ctcctgttag gcagattcct tatctggtga cacaccccca tttcctggag
     7261 ccatctctct ccttgccaga acctctaagg tttgcttacg atggagccag agaggatcct
     7321 gggagggaga gcttggcagg gggtgggagg gaaggggggg atgcgtgacc tgcccggttc
     7381 tcagtggcca ccctgcgcta ccctctccca gaacctgagc tgctctgacg cggctgtctg
     7441 gtgcgtttca ctgatcctgg tgctgcagct tccttacact tcccaagagg agaagcagtt
     7501 tggaaaaaca aaatcagaat aagttggtcc tgagttctaa ctttggctct tcacctttct
     7561 agtccccaat ttatattgtt cctccgtgcg tcagttttac ctgtgagata aggccagtag
     7621 ccagccccgt cctggcaggg ctgtggtgag gaggggggtg tccgtgtgga aaactccctt
     7681 tgtgagaatg gtgcgtccta ggtgttcacc aggtcgtggc cgcctctact ccctttctct
     7741 ttctccatcc ttctttcctt aaagagtccc cagtgctatc tgggacatat tcctccgccc
     7801 agagcagggt cccgcttccc taaggccctg ctctgggctt ctgggtttga gtccttggca
     7861 agcccaggag aggcgctcag gcttccctgt cccccttcct cgtccaccat ctcatgcccc
     7921 tggctctcct gccccttccc tacaggggtt cctggctctg ctctaagggc gaattcgcgg
     7981 ccgctaaatt caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     8041 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     8101 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     8161 gcagcctata cgtacggcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     8221 tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg gtgatccccc
     8281 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     8341 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     8401 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     8461 tgatgttctg gggaatataa atgtcaggca tgagattatc aaaaaggatc ttcacctaga
     8521 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
     8581 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
     8641 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
     8701 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     8761 tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
     8821 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8881 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     8941 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     9001 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     9061 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     9121 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     9181 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     9241 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9301 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     9361 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9421 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9481 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     9541 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9601 gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc
     9661 tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg
     9721 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9781 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9841 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9901 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9961 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10021 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10081 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10141 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10201 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10261 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10321 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10381 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10441 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10501 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10561 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10621 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10681 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10741 ctttttcaat attattgaag catttatcag ggttattgtc tcatgaccaa aatcccttaa
    10801 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10861 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10921 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10981 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    11041 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    11101 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    11161 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    11221 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11281 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11341 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11401 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11461 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11521 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11581 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
