LOCUS       sequence-36245-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, 
            Choi SY
  TITLE     High Cleavage Efficiency of a 2A Peptide Derived from Porcine 
            Teschovirus-1 in Human Cell Lines, Zebrafish and Mice.
  JOURNAL   PLoS One. 2011  . 6(4):e18556.
  PUBMED    21602908
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(4):e18556."
COMMENT     Sequence Label: pSYC-181
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..162
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(207..226)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(208..226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    231..365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(255..274)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     309..328
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(478..498)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(478..496)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(517..533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(517..533)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(530..552)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    541..557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(565..571,572..589,590..595))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    610..631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(748..765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctttgngga ccatcgattg tcagatatct ggcgcgccta ccatggggcc cggccggcct
       61 aagatcaccg gtcccgggct taaggagctc gcatgcggaa gcggagctac taacttcagc
      121 ctgctgaagc aggctggaga cgtggaggag aaccctggac ctagatctgg aattcaaggc
      181 ctctcgagcc tctagatagc tagcctccct atagtgagtc gtattacgta gatccagaca
      241 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      301 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      361 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      421 ttttttaatt cgcggccgcg gcgccaatgc attgggcccg gtacccagct tttgttccct
      481 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      541 ttgttatccg ctcacaattc cacacaacat acgagccggg agcataaagt gtaaagcctg
      601 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      661 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      721 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      781 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      841 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      901 gnccgcgttg ctggcgtttt tccataggct cgccc
//
