LOCUS       sequence-36252-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Citri A, Bhattacharyya S, Ma C, Morishita W, Fang S, Rizo J, Malenka
            RC
  TITLE     Calcium binding to PICK1 is essential for the intracellular 
            retention of AMPA receptors underlying long-term depression.
  JOURNAL   J Neurosci. 2010 Dec 8. 30(49):16437-52.
  PUBMED    21147983
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2010 Dec 8. 30(49):16437-52."
COMMENT     Sequence Label: FuPick-shRNA
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(171..190)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(172..190)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(197..214)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(197..213)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(206..228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        338..737
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ttgggatctt ataagttccc tatcagtgat agggatccct atgagtaccg ccttatcctt
       61 tcaagagaag gataaggcgg tactcatagt tttctagccc caagggcgaa ttctgcagat
      121 atccatcaca ctggcggccg ctcgagcatg catctagagg gcccaattcg ccctatagtg
      181 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      241 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      301 aggcccgcac cgattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg
      361 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
      421 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
      481 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
      541 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
      601 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
      661 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
      721 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
      781 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccg
//
