LOCUS       sequence-36253-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Lindsey-Boltz LA, Wauson EM, Graves LM, Sancar A
  TITLE     The human Rad9 checkpoint protein stimulates the carbamoyl phosphate
            synthetase activity of the multifunctional protein CAD.
  JOURNAL   Nucleic Acids Res. 2004  . 32(15):4524-30.
  PUBMED    15326225
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2004  . 32(15):4524-30."
COMMENT     Sequence Label: pcDNA3.1-HisFlag-CAD
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             148..180
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(184..192,193..207)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             256..279
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             292..315
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggctttagc gactctctgg ctactagaga acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct taccatgggg
      121 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
      181 cgggatctgt acgacgatga cgacaagact agtggatccg attacaaaga cgatgacgac
      241 aagactagtg gatccgatta caaagacgat gacgacaaga ctagtggatc cgattacaaa
      301 gacgatgacg acaagactag tggatccctg gtgttggagg acgggtcggt gctgcagggc
      361 cggccctttg gggccgctgt gtcgactgct ggggaagtgg tgtttcagac cggcatggtc
      421 ggctacccag aggccctcac tgacccttcc tacaaagctc agatcctggt gctcacgtac
      481 cctctcatcg gcaactacgg cattccctca gatgaagagg acgagttcgg cctgagcaag
      541 tggtttgaat cctcggagaa ccacgtggca ggactggtgg tgggagaatg ctgtcccacg
      601 cccagccact ggagtgccac ctgcaccctg catgagtggc tgcagcagca tggcatcccc
      661 ggcctgcaag gagtagacac tcgggagctg actaaaaagt tgcgggagca agggtctctt
      721 ttggggaagc tggtccagag tgggacagag ccttcaaccc tgccgttcgt ggaccccaat
      781 gccaggcccc tggcaccaga ggtttctatt aagaccccac gggtattcaa tgcagggggt
      841 gcccctcgga tctgtgccnt tggactgcgg cctcaagtat aatcagatca gatgtctctg
      901 ccagctttgg ggctgaggtt actgtg
//
