LOCUS       sequence-36260-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Milenkovic L, Scott MP, Rohatgi R
  TITLE     Lateral transport of Smoothened from the plasma membrane to the 
            membrane of the cilium.
  JOURNAL   J Cell Biol. 2009 Nov 2. 187(3):365-74.
  PUBMED    19948480
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Nov 2. 187(3):365-74."
COMMENT     Sequence Label: MSCV-SNAP-Smo
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             216..761
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for mammalian expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 tcttntcnnc ctactccttc tctaggcgcc ggaattagat ctctcgaggt taattcacca
       61 tggccgctgg ccgccccgtg cgtgggcccg agctggcgcc ccggaggctg ctgcagttgc
      121 tgctgctggt actgctgggg ggcccgggcc ggggggcggc cttgagcggg aacgtgaccg
      181 ggcctgggcc tcacagcgcc agcgggagct cgagcatgga caaagactgc gaaatgaagc
      241 gcaccaccct ggatagccct ctgggcaagc tggaactgtc tgggtgcgaa cagggcctgc
      301 acgagatcaa gctgctgggc aaaggaacat ctgccgccga cgccgtggaa gtgcctgccc
      361 cagccgccgt gctgggcgga ccagagccac tgatgcaggc caccgcctgg ctcaacgcct
      421 actttcacca gcctgaggcc atcgaggagt tccctgtgcc agccctgcac cacccagtgt
      481 tccagcagga gagctttacc cgccaggtgc tgtggaaact gctgaaagtg gtgaagttcg
      541 gagaggtcat cagctaccag cagctggccg ccctggccgg caatcccgcc gccaccgccg
      601 ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct gatcccctgc caccgggtgg
      661 tgtctagctc tggcgccgtg gggggctacg agggcgggct cgccgtgaaa gagtggctgc
      721 tggcccacga gggccacaga ctgggcaagc ctgggctggg cagctcgagg aggaacgtgc
      781 cggtgaccag ccctccgccg ccgctgctga gccactgcgg ccgggccgcc cactgcgagc
      841 ctttgcgcta caacgtgtgc ctgggctcgg cgctgcccta cggagccacc accacgctgc
      901 tggctgggga ntcgatcaga aanagaattt tncgncaacc ttga
//
