LOCUS       sequence-362806-       11181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the 
            nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Erdenee E, Ting AY
  TITLE     A Dual-Purpose Real-Time Indicator and Transcriptional Integrator 
            for Calcium Detection in Living Cells.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1086-1095. doi: 
            10.1021/acssynbio.1c00597. Epub 2022 Mar 7.
  PUBMED    35254056
REFERENCE   2  (bases 1 to 11181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00597"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1086-1095"
COMMENT     Sequence Label: NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1
FEATURES             Location/Qualifiers
     source          1..11181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             919..939
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             940..963
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4519..4629
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4636..4677
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4703..5255
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4847..4864)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5074..5093
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5264..5273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5282..5302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5303..5740
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5774..6094
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6689..6715
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(6734..6752)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6735..6752)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6772..6789)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6778..7002
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7048..7476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7135..7154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7345..7366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7485..7505)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7490..7819
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7670..7805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7732..7751
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7886..8680
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7940..7959)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8550..8569
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8854..8975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8891..8910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8945..8964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9024..9040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9024..9040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9037..9059)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9048..9064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9072..9078,9079..9096,9097..9102))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9117..9138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9255..9272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9426..10014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9506..9525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10185..10976,10977..11045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10808..10827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11046..11150)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gccaccatgg gcgcgccgcc caagaagaag aggaaggtgg actacaagga cgatgacgat
      961 aaaggggcta gttcatcttc atctgagaga tggatcgatg gtcttcagtt ctcttccttg
     1021 ttatggcctc cgccacgaga tcctcaacaa cataaggatc aagtcgttgc ttatgttgaa
     1081 tattttggtc aatttacatc agagcaattc ccagatgaca ttgctgagtt ggtccggcat
     1141 cagtatccat caaccgagaa gcgacttttg gacgatgtgc tggcgatgtt tgtccttcat
     1201 catccggagc atggtcatgc agtcattctt ccaatcattt catgtcttat tgatggctcg
     1261 ttggtgtaca gcaaggaagc tcatccgttt gcctctttca tatctttagt ttgcccaagt
     1321 agtgagaatg actattcgga gcaatgggct ttggcatgtg gagaaatcct tcgcattttg
     1381 actcattaca accgtcccat ttataaaact gagcagcaaa atggagatac agagagaaat
     1441 tgtctgagca aagctacaac tagtggttct ccgacttcag agcctaaggc tggatcacca
     1501 acacagcatg aaaggaaacc tttaaggcct ttgtctccat ggatcagtga tatactactt
     1561 gctgctcctc ttggtataag aagtgactat ttccgatggt gtagtggtgt aatgggtaaa
     1621 tatgctgctg gagagctcaa gccgccaacc attgcttctc gaggatctgg taaacatcct
     1681 caactgatgc cttcaacccc aagatgggct gttgctaatg gagctggtgt catactgagt
     1741 gtttgtgatg atgaagttgc tcgatatgag actgctacgc tgacagcggt cgctgtccct
     1801 gcacttcttc ttcctccgcc aacgacatcc ttagatgagc atctagttgc tggccttcca
     1861 gctcttgaac catatgcacg tttgtttcat agatactatg ccattgcaac tccaagtgct
     1921 acgcagagac ttcttcttgg actcttagaa gcaccaccgt cgtgggctcc agatgcactt
     1981 gatgctgctg tacagcttgt ggaactcctt cgagctgctg aagattatgc atctggtgta
     2041 aggctaccca ggaactggat gcatttgcac ttcttgcggg ctataggaat tgctatgtct
     2101 atgagggcag gtgttgctgc tgatgctgca gccgctttgc ttttccgcat actctcacag
     2161 ccggcactgc tttttcctcc gctaagtcaa gttgagggag tagaaattca gcacgcgcct
     2221 attggtggct acagttcaaa ttacagaaaa cagatagaag ttcctgcagc agaagcaacc
     2281 attgaagcca ctgcccaagg aattgcctca atgctttgtg ctcatggtcc tgaagttgag
     2341 tggagaattt gcactatatg ggaagctgct tatggtttga tccctttaaa ttcttcggcg
     2401 gttgatcttc ccgaaatcat agttgctacc ccactgcaac ctcctatctt gtcatggaat
     2461 ttatacattc cactcctcaa agtacttgaa tatcttccac gggggagtcc ttcggaagca
     2521 tgcttgatga aaatatttgt tgccactgtg gaaacaatac tcagtagaac ttttccgcct
     2581 gaatcttcca gggaactaac cagaaaagct agatcgagtt ttaccacaag atcagcgacc
     2641 aaaaatcttg ctatgtctga gcttcgtgct atggtccatg ctctcttttt agaatcatgc
     2701 gctggtgtgg aattagcttc acgcctactt tttgttgtgt tgactgtatg tgttagccat
     2761 gaagcacagt ctagtggtag caagagaccg agaagtgaat atgctagtac tactgaaaat
     2821 attgaggcga atcaacctgt atctaacaat caaactgcta accgtaaaag taggaatgtc
     2881 aagggacagg gacctgtggc agcatttgat tcatacgttc ttgctgctgt ttgtgctctt
     2941 gcctgtgagg ttcagctgta tcctatgatc tctggtgggg ggaacttttc caattctgcc
     3001 gtggctggaa ctattacaaa gcctgtaaag ataaatgggt catctaaaga gtatggagct
     3061 gggattgact cggcaattag tcatacgcgc cgaattttgg caatcctaga ggcactcttt
     3121 tcattaaaac catcttctgt ggggactcca tggagttaca gttctagtga gatagttgct
     3181 gcggccatgg ttgcagctca tatttccgaa ctgttcagac gttcaaaggc cttgacgcat
     3241 gcattgtctg ggttgatgag atgtaagtgg gataaggaaa ttcataaaag agcatcatca
     3301 ttatataacc tcatagatgt tcacagcaaa gttgttgcct ccattgttga caaagctgaa
     3361 cccttggaag cctaccttaa gaatacaccg gttcagaagg attctgtgac ctgtttaaac
     3421 tggaaacaag agaacacatg tgcaagcacc acatgctttg atacagcggt gacatccgcc
     3481 tcaaggactg aaatgaatcc aagaggaaac cataagtatg ctagacattc agatgaaggc
     3541 tcaggaagac cctcagagaa gggtatcaaa gatttcctct tggatgcttc tgatctagcg
     3601 aatttcctca cagctgatag actcgcaggg ttctattgtg gtacacaaaa gcttttgagg
     3661 tcagtgcttg cagagaaacc ggagctgtct ttctccgttg tttcactgtt atggcacaaa
     3721 ctgattgctg ctcctgaaat ccagcccacc gcagaaagca cctctgcgca acaaggatgg
     3781 agacaggttg ttgatgcgct atgcaatgtc gtatctgcaa cgccagcgaa agcagcagca
     3841 gcagttgtcc ttcaggctga aagggagttg cagccttgga tcgccaaaga tgatgaagaa
     3901 ggccaaaaaa tgtggaaaat caaccaacgg atagtcaaag tgttggtgga actcatgcgc
     3961 aatcatgaca ggcctgagtc actggtgatt ctcgcaagtg catcagatct tcttctgcgg
     4021 gcaactgatg gaatgcttgt tgatggagaa gcttgtacat tacctcaact tgagctactt
     4081 gaagccacgg caagagcaat acagccggtg ctagcttggg ggccatctgg actagcagtg
     4141 gtcgacggtt tatccaatct attgaagtgt cgtctaccag caacaatacg gtgcctttca
     4201 cacccaagtg cacacgtacg tgccttaagc acgtcagtac tacgtgatat catgaaccaa
     4261 agctccatac ccatcaaagt aactccaaaa ctgccaacaa cagagaagaa cggaatgaat
     4321 agtccgtcct atcgattctt caacgccgcc tcaatagact ggaaagccga tatccaaaac
     4381 tgtttaaact gggaagctca cagcttgctc tccacaacta tgcctactca gtttctcgac
     4441 actgcggctc gggaactcgg ctgtactata tccttgtccc aagcggccgc aagtgctggt
     4501 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
     4561 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     4621 gacatgctcc ccgggggcaa gcccatcccc aaccccctgc tgggcctgga cagcacctta
     4681 attaactagc cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     4741 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4801 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4861 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4921 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     4981 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5041 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5101 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     5161 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     5221 gacgtggttt tcctttgaaa aacacgatga taatatggcc acagccacca tgggcgcgcc
     5281 gcccaagaag aagaggaagg tgaagctact gtcttctatc gaacaagcat gcgatatttg
     5341 ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa
     5401 caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct
     5461 gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
     5521 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
     5581 aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt
     5641 ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga
     5701 agagagtagt aacaaaggtc aaagacagtt gactgtatcg ccgggcgacc cactagtcca
     5761 gtgtggtgga attggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc
     5821 caagcgcggc cagacctgcg tggtgcacta caccgggatg cttgaagatg gaaagaaatt
     5881 tgattcctcc cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat
     5941 ccgaggctgg gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat
     6001 atctccagat tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac
     6061 tctcgtcttc gatgtggagc ttctaaaact ggaaagcggc agcgagactc ccgggacctc
     6121 agagtccgcc acacccgaaa gtgtcactgg ttatagactc tttgaagaga tactggcggc
     6181 cgcagcgaga gaacatgcga tcggagaagc caccggaaaa cgaaagaaac gcggcagagt
     6241 tgaagaagca gaagaatact gtaacgatgg aatcgaagaa caagtagagg atgagaagct
     6301 tccgttagag gttgggatgt tctattaccc aatgactccg ccttcgttca ttgtttccga
     6361 tgctctggag ccagattttc ctttgatcta tgtcaacaga gtcttcgaag tcttcactgg
     6421 ctatcgtgcc gatgaagttc ttggtcgtaa ctgtcgattc ctacagtaca gagatcctcg
     6481 agctcaaagg cgtctcccat tggttgatcc tgtggttgta tctgagatta ggagatgtct
     6541 tgaagaaggt attgaattcc aaggagagct tcttaatttc agaaaagatg gtactccttt
     6601 ggttaacaga ctacggcttg ctccaatacg tgacgatgat ggaaccatta cacacgtaat
     6661 tgggatacag gtcttctctg aaacggctta cccatacgat gttccagatt acgcttagtc
     6721 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     6781 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6841 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     6901 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6961 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     7021 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     7081 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7141 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7201 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7261 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7321 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7381 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7441 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     7501 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7561 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7621 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7681 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7741 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     7801 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     7861 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     7921 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     7981 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     8041 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     8101 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     8161 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     8221 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     8281 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     8341 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     8401 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     8461 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     8521 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     8581 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     8641 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     8701 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     8761 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8821 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     8881 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8941 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     9001 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9061 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9121 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9181 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9241 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9841 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9901 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9961 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10021 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10081 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgacttccc
    10261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10501 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10801 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11161 cgaaaagtgc cacctgacgt c
//
