LOCUS       sequence-36282-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Lindsey-Boltz LA, Sercin O, Choi JH, Sancar A
  TITLE     Reconstitution of human claspin-mediated phosphorylation of Chk1 by 
            the ATR (ataxia telangiectasia-mutated and rad3-related) checkpoint 
            kinase.
  JOURNAL   J Biol Chem. 2009 Nov 27. 284(48):33107-14.
  PUBMED    19828454
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Nov 27. 284(48):33107-14."
COMMENT     Sequence Label: pGEX-4T1-Claspin-N
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caatcggact ggttccgcgt ggatccacca tgcatcacca tcaccatcac acaggcgagg
       61 tgggttctga ggttcaccta gaaatcaatg acccaaacgt catttcacaa gaggaagcag
      121 atagtccttc agatagtgga cagggcagct atgaaacaat tggacccttg agtgaaggag
      181 attcagatga agagatattt gtaagtaaga agttgaaaaa caggaaggtt ctacaagaca
      241 gtgattccga aacagaggac acaaatgcct ctccagagaa aactacctat gacagtgccg
      301 aggaggaaaa taaagagaat ttatatgctg ggaaaaatac aaaaatcaaa aggatttaca
      361 aaactgtggc agacagtgat gaaagttaca tggaaaagtc tttgtatcag gaaaatcttg
      421 aagcgcaagt gaaaccttgc ttagagctga gtcttcagtc tggaaactct acagacttta
      481 ccactgacag aaagagttcc aaaaagcaca tacatgataa agaaggaact gcaggaaaag
      541 caaaagtaaa atcaaaaaga agacttgaga aagaggagag aaaaatggaa aaaattagac
      601 agctaaaaaa gaaggaaaca aaaaaccagg aagatgatgt agaacagcca tttaatgaca
      661 gtggctgtct tcttgtggat aaagaccttt ttgaaactgg gttggaggat gaaaataact
      721 ctccattgga agatgaagag tcattagaat caataagagc agctgtaaaa aacaaagtaa
      781 aaaagcacaa gaaaaaaaga accatctttg gagagtgggg tccattcatt tgaggggagg
      841 aagtgagtta tcnaaagnaa ccacgaggga agggaaagaa aggccagcca gattaagtaa
      901 agaagcatta aaaccacctg catagtgaga ncacnctatc nnttttcgag agtcntgccc
      961 cgtgaacctt ccattncata tgccnggaga
//
