LOCUS       sequence-362899-        7510 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Rosalind Kim, Steve J. Sandler, Stanley Goldman, Hisao Yokota, Alvin
            J. Clark, Sung-Hou Kim
  TITLE     Overexpression of archaeal proteins in Escherichia coli
  JOURNAL   Biotechnology Letters. March 1998. 20(3): 207-210.
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechnology Letters. March 1998. 20(3): 207-210."
COMMENT     Sequence Label: pSJS 1240
FEATURES             Location/Qualifiers
     source          1..7510
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     tRNA            complement(139..214)
                     /gene="ileX"
                     /product="E. coli tRNA-Ile recognizing AUA"
                     /label=ileX
     tRNA            461..537
                     /gene="argU"
                     /product="E. coli tRNA-Arg recognizing AGA and AGG"
                     /label=argU
     CDS             complement(2627..3415)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(5580..6239)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6154..6173
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6240..6342)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6734..6751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6868..7413)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6948..6967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtaattctc atgtttgaca gcttatcatc gataagcttg gtaccgagct cggatccact
       61 agtaacggcc gccagtgtgc tggaattcgg cttaaagctt aaaaaggctg acgatttctc
      121 gtcagccttt gcggccgctg gtggcccctg ctggacttga accagcgacc aagcgattat
      181 gagtcgcctg ctctaaccac tgagctaagg ggccaatcga ttggattata aagtaactcc
      241 gtgtcgcaat ccaagcttgg ctgcaggtca ctttgataca tgaaaatacg ggttttcttg
      301 attcagacgc gcagcggtgt gcgtttgttt gccgctatag cgaaataaat cagaaaatca
      361 gacgcggtcg ttcacttgtt cagcaaccag atcaaaagcc attgactcag caagggttga
      421 ccgtataatt cacgcgatta caccgcattg cggtatcaac gcgcccttag ctcagttgga
      481 tagagcaacg accttctaag tcgtgggccg caggttcgaa tcctgcaggg cgcgccatta
      541 caattcaatc agttacgcct tctttatatc ctccataatt tcagagtggg acatatttgg
      601 gacattatca ccaaaaatgt cgtctatttt cctcgcatgc gagctcgacc tgcttgctat
      661 ggcgcattca tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat
      721 ctagcctcta tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac
      781 agtctcatcg aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta
      841 gagacacaaa cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc
      901 ggcggcattg acctgttcac gtataaaact agacctcaag tctcgaacga aacagcgatg
      961 tactggtact ggttctcggg agcacaggat gacgcctaac aattcattca agccgacacc
     1021 gcttcgcggc gcggcttaat tcaggagtta aacatcatga gggaagcggt gatcgccgaa
     1081 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     1141 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     1201 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     1261 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     1321 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     1381 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     1441 attgatcgtt actattaata gatattgata gacttccaag aacgccataa ccgatcatta
     1501 tccatgatgg ggcgcatgtc gcangcgatt agtctgagca ttatatacac gtgaggccgc
     1561 ggacgggtga tgcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     1621 acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgckgccc gagatgcgcc
     1681 gcgtgcggck gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg
     1741 cattcacagt yctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
     1801 cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa
     1861 cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt
     1921 gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct
     1981 ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
     2041 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
     2101 gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc
     2161 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     2221 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     2281 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     2341 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     2401 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg
     2461 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     2521 aaggaatggt gcatgcgagc tcgacccgca tagtcttccc cagctctcta acgcttgagt
     2581 taagccgcgc cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta
     2641 ccttggtgat ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg
     2701 ccaagcgatc ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact
     2761 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     2821 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     2881 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     2941 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     3001 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     3061 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     3121 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     3181 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     3241 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     3301 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     3361 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt
     3421 ttaactcctg aattaagccg cgccgcgaag cggtgtcggc ttgaatgaat tgttaggcgt
     3481 catcctgtgc tcccgagaac cagtaccagt acatcgctgt ttcgttcgag acttgaggtc
     3541 tagttttata cgtgaacagg tcaatgccgc cgagagtaaa gccacatttt gcgtacaaat
     3601 tgcaggcagg tacattgttc gtttgtgtct ctaatcgtat gccaaggagc tgtctgctta
     3661 gtgcccactt tttcgcaaat tcgatgagac tgtgcgcgac tcctttgcct cggtgcgtgt
     3721 gcgacacaac aatgtgttcg atagaggcta gatcgttcca tgttgagttg agttcaatct
     3781 tcccgacaag ctcttggtcg atgaatgcgc catagcaagc aggtcgagct cgcatgcacc
     3841 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     3901 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     3961 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     4021 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     4081 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     4141 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     4201 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     4261 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     4321 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     4381 tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc
     4441 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
     4501 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg
     4561 cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact
     4621 gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc
     4681 cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc
     4741 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     4801 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     4861 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcccctacg
     4921 tgctgctgaa gttgcccgca acagagagtg gaaccaaccg gtgataccac gatactatga
     4981 ctgagagtca acgccatgag cggcctcatt tcttattctg agttacaaca gtccgcaccg
     5041 ctgccggtag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     5101 tcttctttat catgcaactc gtaggacagg tgccggcagc gcccaacagt cccccggcca
     5161 cggggcctgc caccataccc acgccgaaac aagcgccctg caccattatg ttccggatct
     5221 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
     5281 aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa ttattacctc
     5341 cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct
     5401 tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg
     5461 ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta
     5521 tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc ttaaaaaaat
     5581 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     5641 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     5701 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     5761 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     5821 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     5881 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     5941 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     6001 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga
     6061 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     6121 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     6181 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     6241 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     6301 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa
     6361 aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag
     6421 tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta
     6481 ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg
     6541 tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca
     6601 gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg
     6661 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga
     6721 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     6781 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg
     6841 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca
     6901 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
     6961 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     7021 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     7081 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
     7141 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag
     7201 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa
     7261 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag
     7321 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag
     7381 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa
     7441 tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
     7501 cgatataagt
//
