LOCUS       sequence-362929-        6220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmLSm1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Tritschler F, Eulalio A, Truffault V, Hartmann MD, Helms S, Schmidt 
            S, Coles M, Izaurralde E, Weichenrieder O
  TITLE     A divergent Sm fold in EDC3 proteins mediates DCP1 binding and 
            P-body targeting.
  JOURNAL   Mol Cell Biol. 2007 Dec . 27(24):8600-11.
  PUBMED    17923697
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Dec . 27(24):8600-11."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmLSm1_A
FEATURES             Location/Qualifiers
     source          1..6220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2325..2327,2328..2330,2331..3041)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2325..2327,2328..2330,2331..3041)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2370..2391)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2631..2650)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2978..2999
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3507..3548
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3558..3575
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3598..3615)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3648..3782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3672..3691)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3726..3745
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4383..4971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4463..4482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5142..5933,5934..6002))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5765..5784
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6003..6107)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6175..6193
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 attctaaaaa cacaaatgat acttctaaaa aaaaatcatg aatggcatca actctgaatc
       61 aaatctttgc agatgcacct acttctcatt tccactgtca catcattttt ccagatctcg
      121 ctgcctgtta tgtggcccac aaaccaagac acgttttatg gccattaaag ctggctgatc
      181 gtcgccaaac accaaataca taatgaatat gtacacattc gagaaagaag cgatcaaaga
      241 agcgtcttcg ggcggagtag gagaatgcgg aggagaagga gaacgagctg atctagtatc
      301 tctccacaat ccaatgccaa ctgaccaact ggccatattc ggagcaattt gaagccaatt
      361 tccatcgcct ggcgatcgct ccattcttgg ctatatgttt ttcaccgtta cccggggcca
      421 ttttcaaaga ctcgtcggca agataagatt gtgtcactcg ctgtctctct tcatttgtcg
      481 aagaatgctg aggaatttct gcgtcttttg ccgaacgcag tgcatgtgca attgttgttt
      541 tttggaatat tcaattttcg gactgtccgc tttgatttca gtttcttggc ttattcaaaa
      601 agcaaagtaa agccaaaaaa gcgagatggc aataccaaat gcggcaaaac ggtagtggaa
      661 ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg gcgtggcggg gtctgtggct
      721 gggcgcgacg tcaccgacgt tggagccact cctttgacca tgtgtgcgtg tgtgtattat
      781 tcgtgtctcg ccactcgccg gttgtttttt tctttttatg ctgcgctctc tctagcgcca
      841 tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt ccttttatgc gtcattttgg
      901 ctcgaaatag gcaattattt aaacaaagat tagtcaacga aaacgctaaa ataaataagt
      961 ctacaatatg gttacttatt gccatgtgtg tgcagccaac gatagcaaca aaagcaacaa
     1021 cacaggtggc tttccctctt tcactttttg tttgcaagcc gcgtgcgagc aagacggcac
     1081 gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag ttttggcgaa aaacatcaag
     1141 gcgcctgata cgaatgcatt tgcaataaca attgcgatat ttaatattgt ttatgaagct
     1201 gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat tatttgaaag agaattagga
     1261 atcggacgct tatcgttagg gtaacaacaa gaaatgctta ctgagtcaca gcctctggaa
     1321 aactgccgca agccagagag agagagaaaa agagggagag cagcttagac cgcatgtgct
     1381 tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa cgcatagact gcactgaaaa
     1441 aatcgattac ctatttttta tgaatgaata tttgcactat tactattcaa aactattaag
     1501 atagcaatca cattcaatag ccaaatacta taccacctga gcgatgcaac gaaatgatca
     1561 atttgagcaa aaatgctgca tatttaggac ggcatcatta tagaaatgct tcttgctgtg
     1621 tacttttctc tcgtctggca gctgtttcgc cgttattgtt aaaaccggct taagttaggt
     1681 gtgttttcta cgactagtga atgccctact agaagatgtg tgttgcacaa aatgtccctg
     1741 gaataaccaa tttgaagtgc agatagcagt aaacgtaagc taatatgaat attatttaac
     1801 tgtaatgttt taatatcgct ggacattact aataaaccca ctataaacac atgtacatat
     1861 gtatgttttg gcatacaatg agtagttggg gaaaaaatgt gtaaaagcac cgtgaccatc
     1921 acagcataaa gataaccagc tgaagtatcg aatatgagta acccccaaat tgaatcacat
     1981 gccgcaactg ataggaccca tggaagtaca ctcttcatgg cgatatacaa gacacacaca
     2041 agcacgaaca cccagttgcg gaggaaattc tccgtaaatg aaaacccaat cggcgaacaa
     2101 ttcataccca tatatggtaa aagttttgaa cgcgacttga gagcggagag cattgcggct
     2161 gataaggttt tagcgctaag cgggctttat aaaacgggct gcgggaccag ttttcatatc
     2221 actaccgttt gagttcttgt gctgtgtgga tactcctccc gacacaaagc cgctccatca
     2281 gccagcagtc gtctaatcca gagaccccgg atcggggtac catcatggtg agcaagggcg
     2341 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     2401 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     2461 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     2521 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     2581 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     2641 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2701 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2761 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     2821 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2881 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2941 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3001 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gccaagcttg aattctatgg
     3061 acgacttaaa tccgctggcg ggcacggctc acctcctgga agaggttgac aagaaactga
     3121 tggtactttt gagagacgga cggactctga ttggatacct gcggtccgtg gaccagttcg
     3181 ccaacctggt gctgcaacgc accatcgagc ggatacatgt gggcaacgaa tacggcgaca
     3241 ttcctcgtgg agtcttcatc attcgcggcg agaatgtggt gctactgggc gaaatagacc
     3301 gtgaaaagga gcagaaactg ccactcaaag agatatccgt cgatgaaatc ctggacgccc
     3361 agcgtaggga acaggagcag cggcaggaga aacaccgcct agtatccaag gcactaaagg
     3421 aacgaggcct ggccgtagat gccaacataa tcaacgagga cttctgctaa ggcggccgct
     3481 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     3541 attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     3601 cgactgtgcc ttctaaggcc tgagctcgct gatcagcctc gatcgaggat ccagacatga
     3661 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3781 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     3841 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag tcgaccgatg
     3901 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3961 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     4021 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     4081 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     4141 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     4201 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     4261 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     4321 cagggacagc ttcaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4381 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4441 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4501 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4561 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4621 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4681 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4741 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4801 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4861 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4921 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4981 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5041 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5101 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5161 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5221 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5281 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5341 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5401 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
     5461 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5521 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5581 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5641 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5701 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     5761 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5821 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5881 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5941 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6001 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6061 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6121 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     6181 cgtatcacga ggccctttcg tcttcaagaa ttaattctat
//
