LOCUS       sequence-362936-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro transcription of heiCas9 mRNA - mammalian codon 
            optimized Cas9, N-terminal hei-tag (Myc-flexible 
            linker-optimizedNLS) and C-terminal oNLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Thumberger T, Tavhelidse-Suck T, Gutierrez-Triana JA, Cornean A, 
            Medert R, Welz B, Freichel M, Wittbrodt J
  TITLE     Boosting targeted genome editing using the hei-tag.
  JOURNAL   Elife. 2022 Mar 25;11. pii: 70558. doi: 10.7554/eLife.70558.
  PUBMED    35333175
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-25"; volume: "11. pii: 70558. doi"; pages: " 
            10.7554/eLife.70558"
COMMENT     Sequence Label: pCS2+ heiCas9
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tacgactcac tatagttcta gattagtcca gcctgggcct cttgggagga ggggatccgt
       61 cacccccaag ctgtgacagg tcgattctgg tttcgtacag gccggtgatg ctctggtgga
      121 tcagggtggc gtccagcacc tccttggtgc tggtgtatct ctttctgtcg attgtggtgt
      181 cgaagtactt gaaggctgcg ggtgcgccca ggttggtcag ggtgaacagg tgaatgatgt
      241 tctcggcctg ctctctgatg ggcttgtctc tgtgcttgtt gtaggcgctc agcaccttgt
      301 ccaggttggc gtcagccagg atcactctct tactgaactc gctgatctgc tcaatgattt
      361 cgtccaggta gtgcttgtgc tgctccacga acagctgctt ctgctcgttg tcctcggggc
      421 tgcccttcag cttctcgtag tggctggcca ggtacaggaa gttcacgtac ttgctgggca
      481 gggccagctc gttgcccttc tgcagctcgc cggcgctggc cagcattctc tttctgccgt
      541 tctccagctc gaacaggctg tacttgggca gcttgataat caggtctttc ttcacctcct
      601 tgtagccctt ggcctccagg aagtcgatgg ggttcttctc gaagctggat ctctccatga
      661 tggtgatgcc caggagctcc ttcacgctct tcagtttctt gctcttgccc ttctccacct
      721 tggccacgac cagcacgctg taggccacgg tggggctgtc gaagcctccg tatttcttgg
      781 ggtcccagtc tttctttctg gcgatcagct tgtcgctgtt tctcttgggc aggatgctct
      841 ccttgctgaa gcctccggtc tgcacctcgg ttttcttcac gatgttcacc tggggcatgc
      901 tcagcacctt tctcacggtg gcgaagtctc tgcccttgtc ccacacgatc tcgccggttt
      961 cgccgttggt ttcgatcagg ggcctctttc tgatctcgcc gttggccagg gtgatctcgg
     1021 tcttaaagaa gttcatgatg ttgctgtaaa agaagtactt ggc
//
