LOCUS       sequence-363296-        8735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded FRET-based PKA activity reporter fused to 
            AKAP79..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Tenner B, Getz M, Ross B, Ohadi D, Bohrer CH, Greenwald E, Mehta S, 
            Xiao J, Rangamani P, Zhang J
  TITLE     Spatially compartmentalized phase regulation of a Ca(2+)-cAMP-PKA 
            oscillatory circuit.
  JOURNAL   Elife. 2020 Nov 17;9. pii: 55013. doi: 10.7554/eLife.55013.
  PUBMED    33201801
REFERENCE   2  (bases 1 to 8735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-17"; volume: "9. pii: 55013. doi"; pages: " 
            10.7554/eLife.55013"
COMMENT     Sequence Label: pcDNA3-AKAP79-AKAR4
FEATURES             Location/Qualifiers
     source          1..8735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      895..904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             907..936
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             949..960
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(2251..2253,2254..2256,2257..2967)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3490..4224
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     promoter        complement(4288..4306)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4289..4306)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4326..4343)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4332..4556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4602..5030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4689..4708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4899..4920
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5039..5059)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5044..5373
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5224..5359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5286..5305
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5440..6234
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5494..5513)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6104..6123
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6408..6529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6445..6464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6499..6518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6578..6594)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6578..6594)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6591..6613)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6602..6618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6626..6632,6633..6650,6651..6656))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6671..6692
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6809..6826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6980..7568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7060..7079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7739..8530,8531..8599))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8362..8381
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8600..8704)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
      901 atgggccatc atcatcatca tcatcatcat catcacagca gcggccatat cgaaggtcgt
      961 catatggaaa ccacaatttc agaaattcat gtagaaaaca aggatgagaa gagatcagca
     1021 gaaggtagtc ctggggctga aaggcagaag gaaaaggcat ccatgctttg cttcaagaga
     1081 agaaagaaag cagccaaagc actgaagccc aaagctggct ctgaagctgc tgatgtggca
     1141 aggaagtgtc cacaagaagc aggagcttct gatcagccag agcccacacg gggggcctgg
     1201 gcctcactca aacgtcttgt aacacgcagg aaaaggtcag agtcttcaaa gcagcaaaag
     1261 ccattggagg gtgaaatgca acctgcaata aatgctgagg atgctgatct ttctaagaaa
     1321 aaggcaaaat ctagacttaa gattccctgc ataaaattcc caagagggcc aaaaaggagt
     1381 aatcattcca aaattataga agactcagac tgcagcatca aagtccagga agaagctgaa
     1441 attttggata tacaaacaca gaccccattg aatgatcagg caacaaaggc taagtcaacc
     1501 caggatctaa gtgaaggcat ctcacagaaa gatggtgatg aggtctgtga atcaaatgtg
     1561 agcaatagca taacttctgg agagaaagtg atttcagtag aacttggatt agataatggg
     1621 cattctgcta ttcaaacggg aactctaatc cttgaagaaa ttgaaacgat caaggaaaaa
     1681 caagatgttc aaccccagca agcaagccca cttgaaactt cagaaacaga ccatcagcag
     1741 ccagtacttt ctgatgttcc tcctttacct gcaattccag atcaacaaat tgtggaagaa
     1801 gccagtaaca gtaccctaga aagtgcacca aatggaaaag actatgaaag tacagagatt
     1861 gtagctgaag aaactaagcc aaaagatact gaattgagcc aagaatcaga ttttaaagaa
     1921 aatgggatca ctgaagagaa atccaaatca gaagaaagca aaagaatgga gccaattgct
     1981 attattatta cagacactga aatcagtgaa tttgatgtta caaaatctaa aaatgtccct
     2041 aagcaattct taatttcagc tgaaaatgag caagtagggg tttttgctaa tgataatggt
     2101 tttgaggata gaacttcaga acaatatgaa acactcttaa ttgaaacagc ctcttctcta
     2161 gtcaagaatg ctattcagtt gtcaatagaa cagctggtta atgaaatggc ctctgatgat
     2221 aataaaataa acaatcttct acaggatccc atggtgagca agggcgagga gctgttcacc
     2281 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacag gttcagcgtg
     2341 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2401 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
     2461 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2521 gaaggctacg tccaggagcg taccatcttc ttcaaggacg acggcaacta caagacccgc
     2581 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2641 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacgccat cagtgacaac
     2701 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg cccacttcaa gatccgccac
     2761 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2821 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2881 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2941 actctcggca tggacgagct gtacaagcgc atgcataagt tttctcaaga acagatcggc
     3001 gaaaacattg tgtgcagggt catttgtacc acgggtcaaa ttcccatccg agatttgtca
     3061 gctgatattt cacaagtgct taaggaaaaa cgatccataa agaaagtttg gacatttggt
     3121 agaaacccag cctgtgacta tcatttagga aacatttcaa gactgtcaaa taagcatttc
     3181 caaatactac taggagaaga cggtaacctt ttattgaatg acatttccac taatgggacc
     3241 tggttaaatg ggcaaaaagt cgagaagaac agcaatcagt tactgtctca aggtgatgaa
     3301 ataaccgttg gtgtaggcgt ggaatcagat attttatctc tggtcatttt cataaacgac
     3361 aaatttaagc agtgcctcga gcagaacaaa gttgatcgct ctgcaggtaa gccaggcagc
     3421 ggcgagggca gcaccaaggg cctgcgtcgc gccaccctgg tggacggcgg caccggcggc
     3481 agcgagctca tgggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     3541 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa
     3601 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     3661 actctcggca tggacgagct gtacaaggga ggtaccggtg gatctatggt gagcaagggc
     3721 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3781 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3841 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3901 ggctacggcc ttcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     3961 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4021 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4081 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4141 tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     4201 ttcaagatcc gccacaacat cgagtaagaa ttctgcagat atccatcaca ctggcggccg
     4261 ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
     4321 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4381 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4441 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4501 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4561 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4621 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4681 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4741 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4801 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4861 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4921 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4981 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5041 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5101 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5161 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5221 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5281 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5341 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5401 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5461 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5521 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5581 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5641 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5701 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5761 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5821 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5881 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5941 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     6001 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     6061 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     6121 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     6181 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     6241 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6301 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6361 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6421 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6481 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6541 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6601 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6661 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6721 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6781 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6841 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6901 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6961 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7141 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7321 cactggcagc agccactggt aacaggatta gcaaagcgag gtatgtaggc ggtgctacag
     7381 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7741 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7801 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7861 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7921 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7981 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8041 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8101 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8161 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8221 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8281 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8341 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8401 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8461 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8521 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8581 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8641 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8701 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
