LOCUS       sequence-363549-        6992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP tagged zebrafish cdh1 lacking the cytoplasmic domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Boutillon A, Escot S, Elouin A, Jahn D, Gonzalez-Tirado S, Starruss 
            J, Brusch L, David NB
  TITLE     Guidance by followers ensures long-range coordination of cell 
            migration through alpha-catenin mechanoperception.
  JOURNAL   Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 
            10.1016/j.devcel.2022.05.001.
  PUBMED    35613615
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2022 May 17. pii: S1534-5807(22)00329-X. doi: 
            10.1016/j.devcel.2022.05.001."
COMMENT     Sequence Label: pCS2+ Zf cdh1-deltaCyot-GFP
FEATURES             Location/Qualifiers
     source          1..6992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..67
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     49..66
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2298..2300,2301..2303,2304..3017)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2298..2300,2301..2303,2304..3014)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2343..2364)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2604..2623)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2951..2972
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3058..3192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3082..3101)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3136..3155
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3305..3325)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3305..3323)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3344..3360)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3344..3360)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3357..3379)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3368..3384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3392..3398,3399..3416,3417..3422))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3437..3458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3575..3592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3746..4334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3826..3845)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4505..5296,5297..5365))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5128..5147
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5366..5470)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5496..5951)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5633..5654)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5845..5864
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(6022..6992,1..14)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 ctcgtttagg gaaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca
       61 ctatagaata caagctactt gttctttttg caggatccgg atccgccacc atggcctgcg
      121 tgaccacagt gggactggga gtgatcttct tcctgttccg cgtgttcagc agcggataca
      181 cccacatgag catctgcacc cctggattcg agctggaaga gttcgtgttc aaggtgcaca
      241 gaaaccatct gcacagcggc aagagactgg gaaaagtgac cttcaacaac tgcgacggaa
      301 gaaccagaac cctgttccag agcatcgaca agagattcga gatcaacacc gacggaaccg
      361 tgaccctgaa gagacaggtc acactgcacg agggacacaa ggtgttctct gtgcatgcct
      421 gggacagcag cggaatgaag cacaccgcca gcgtgcgcgt cgagcgcgtg ccagctcagg
      481 tcgagagcag cagcgacgtg gacctgacca agaacaagcg cgtgaagaga ggatggatca
      541 tccctcctat cagcgtgtcc gagaacagca agggaccttt cccaatgaga ctggtgcaga
      601 tcaagagcga ctacgccatc gagacaagac tggcctacaa gatcaccggc gagggcgccg
      661 acctggaccc taagggaatc ttcaccatcg acagactgag cggatgggtg tccgtgaccc
      721 agctgctgga cagagagaag aaggccagct acaagctgag agcccacgcc aacggcgtgg
      781 acgccgacgt gaccgagaag cctatggaca tcatcgtgac cgtgaccgac cagaacgaca
      841 acaagcctgt gttcacccag aaccctttca acggaaacgt gccagaagct ttggagaagg
      901 gcgaggtgtt catgaccgtg acagccaccg acgccgacga caaagagaac accgacaacg
      961 ccgacatcag ctatgtgatc atcagccagg accctccaag ccctaagcct aacatgttcg
     1021 ccatcaaccc tgtgtctggc ggaatcagcg tgctggaaaa gggactggat agagagcagt
     1081 ggttcagata caccctggtg atcaccgcca ccgacatgaa cggcgaggga ctgagcacca
     1141 ccggaaccgc cgtgatcacc gtgacagact ctaacgacaa cgcccctctg ttcgagcaga
     1201 gcagctacac agctagcgtg ccagagaacc aggtcggagt cgaggtggcc aagctgcctg
     1261 tgaccgacgg tgacgagcct gagagcaccg cttggagcac caagtaccag atcattgccg
     1321 gcgacaaggg cggattcttc aacatcagca ccggacctag cagactggaa ggcatcatca
     1381 caaccgtgaa gcctctggac tacgagaaaa caaagcagta catcctgagc gtgatcgtgg
     1441 tgaacgacga caagttcgtg ggacctctgc ctaccagcac cgccacagtg accgtgaacg
     1501 tgaaggacgt gaacgagcct cctgagttca tccccaaaga gaagttcatc agcagacctg
     1561 agaacctgcc tgtgggaagc aacctgatcc ctttcaccgc catcgaccct gacaccgaga
     1621 agaagcagaa catcacctac agaatcggaa acgaccctag cgactggctg aacatcaccg
     1681 gaagcggaca gatccaggtc aaaaacgccc tggaccgcga gagcagcaac gtgaaagacg
     1741 gaaagtacaa ggccctgatc ctggccctgg acaacgacgt ggaaagccct gctaccggaa
     1801 ccggaacact ggtgatcgag ctgcaggatg tgaacgataa cgctcctgtg atcaacgaga
     1861 gaaccatcaa gctgtgcaac cgcgagtctg cccctgtgct gctgagcatt accgacaagg
     1921 acctgcctcc tttcgccgga cctttcaagg tcgagcctca gggcgacacc agcaagaatt
     1981 ggagcgtgtt cttcaacgag acaggccact tcctgaacat caagcctcag agccagctgg
     2041 aacagggcga gtacaaggtg gtgctgagag tggccgacag agagggcgag agccaagaga
     2101 acatcatcca ggccagcgtg tgcgactgca agggcgaggc cttccagtgc accgacaaac
     2161 aggtggccgg aatcccactg tttggagtgc tgggcgtgct gggaggaatc cttctcctgc
     2221 tgctgctggc tttgctgctg ctgatgttcc tgagaagaaa gagcaacagc aagaaagagc
     2281 ctctgctgca attcaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     2341 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     2401 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     2461 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     2521 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     2581 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2641 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2701 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     2761 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     2821 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2881 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2941 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     3001 acgagctgta caagtaaagc ctcgagcctc tagaactata gtgagtcgta ttacgtagat
     3061 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3121 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3181 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     3241 tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt
     3301 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     3361 tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc ataaagtgta
     3421 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3481 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3541 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3601 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3661 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3721 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3781 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3841 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3901 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3961 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4021 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4081 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4141 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4201 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4261 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4321 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4381 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4441 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4501 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4561 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4621 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4681 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4741 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4801 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4861 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4921 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4981 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5041 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5101 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5161 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5221 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5281 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5341 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5401 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5461 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     5521 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     5581 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     5641 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     5701 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     5761 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     5821 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     5881 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     5941 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     6001 ggcctcttcg ctattacgcc agtcgaccgc caattcaata tggcgtatat ggactcatgc
     6061 caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc
     6121 caattcaata tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc
     6181 caattcaata tggcggacct ggcactgtgc caactgggga ggggtctact tggcacggtg
     6241 ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg
     6301 ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat
     6361 ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata
     6421 tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc
     6481 atataccata tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg
     6541 gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc
     6601 gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt
     6661 caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc
     6721 tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct
     6781 attaatagta acttggcaag tacattacta ttggaagtac gccagggtac attggcagta
     6841 ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg
     6901 acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag
     6961 gcgtgcctaa tgggaggtct atataagcaa tg
//
