LOCUS       sequence-363665-        5042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: RRP1_pCSdest
FEATURES             Location/Qualifiers
     source          1..5042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     138..155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    272..293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(308..313,314..331,332..338)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    346..362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     351..373
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     370..386
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     370..386
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     405..425
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        407..425
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    538..672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(575..594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     629..648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    716..740
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1635..1659)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(1713..1731)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1714..1731)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1766..2750)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     rep_origin      2821..3276
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2908..2927)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3118..3139
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3302..3406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3407..3475,3476..4267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3625..3644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4438..5026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4927..4946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
       61 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      121 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      181 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
      241 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      301 cccaggcttt acactttatg ctcccggctc gtatgttgtg tggaattgtg agcggataac
      361 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
      421 aaagggaaca aaagctgggt accgggccca atgcattggc gccgcggccg cgaattaaaa
      481 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
      541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
      661 catgtctgga tctacgtaat acgactcact atagttctag aggctcgaga ggatcaccac
      721 tttgtacaag aaagctgggt tctagtgagg tgctaacatg tgaggattaa tccagtgatt
      781 ccggtcacaa tgcattccag gaggaggtac ccatgtcact ggaattgggc gatatggttt
      841 attttttctt ccctgatttg gataaccaaa tggaacagga ggaggatagt gattctgatg
      901 gccattccct cgatacattc ctggcttttt tctgggcaaa gggtgccaca ttggaagagg
      961 tggaaatata agttctgaaa tctggtacac aggacttgcg gctgcagtca ccgaactggg
     1021 tttcacttcc atttccttac ttgtttcttc atctgaagaa gaggatcctg aatgataatc
     1081 agaggtaacc ttatcaaggg ccctggtaac tttcctggag atctcatcct tgttctcatc
     1141 tttattttca aagctggcaa caatgaatgc attgtttttc tctccataca taatcaaaaa
     1201 ggaggaggcc gggcgcagtg gctcacgcct gtaatccagc gctttgggag gccaaggcgg
     1261 gcggatcacg aggtcaggag tttgagagca gtctggccaa catggtgaaa ccccatctct
     1321 acgacagcac acctcacatg ggtccaccca gagagtgagc tcctttggca agcccaggtc
     1381 actatacaag atgcagctgt tttcacaggc tttcaggaca tcaggatcaa ctctctgaaa
     1441 tttattgaca cgaatacatc tgtaggcctg tcctttcgat ggtttttctg gataccagtg
     1501 atttttatat ttttcttgaa gtattagggt caatttctca gcaaacctct caactgcctc
     1561 ttttttcaac ttatcatgtt ttcgaactag ccttgtgaaa aagaagacaa cggcagcaat
     1621 ttcattcttc atggtgcctg cttttttgta caaacttgtg atccttgaat tcgaatcgat
     1681 gggatcctgc aaaaagaaca agtagcttgt attctatagt gtcacctaaa tcaagcttgc
     1741 tccgacgtcc ccaggcagaa tggcggttcc ctaaacgagc attgcttata tagacctccc
     1801 attaggcacg cctaccgccc atttacgtca atggaacgcc catttgcgtc attgcccctc
     1861 cccattgacg tcaatgggga tgtacttggc agccatcgcg ggccatttac cgccattgac
     1921 gtcaatggga gtactgccaa tgtaccctgg cgtacttcca atagtaatgt acttgccaag
     1981 ttactattaa tagatattga tgtactgcca agtgggccat ttaccgtcat tgacgtcaat
     2041 agggggcgtg agaacggata tgaatgggca atgagccatc ccattgacgt caatggtggg
     2101 tggtcctatt gacgtcaatg ggcattgagc caggcgggcc atttaccgta attgacgtca
     2161 atgggggagg cgccatatac gtcaatagga ccgcccatat gacgtcaata ggaaagacca
     2221 tatatagaga ccattgacgt caatggggga gtggctatgg gcggtattag gaagccccat
     2281 atatggtata tgggaccgcc cattgggagg ggctatctac gtcaatagga aaacccatat
     2341 atggaatact atatggcata gggccaatac atagtattga acctggccaa tagccatatt
     2401 ggcatagggc catattggat attgcctata tattgatcct ggcatatagc caatatggcc
     2461 gccattattg gcaccatgcc aattcaatat ggcggacttg gcacagggcc aagacccctc
     2521 ctcaaacttg gcaccgtgcc aagtagaccc ctccccagtt ggcacagtgc caggtccgcc
     2581 atattgaatt ggcatggtgc caagtccgcc atattgaatt ggctggggtc cagatccacc
     2641 atattgaatt ggcacgagtc catatacgcc atattgaatt ggcacaggtc cagatccacc
     2701 atattgaatt ggcatgagtc catatacgcc atattgaatt ggcggtcgac tggcgtaata
     2761 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     2821 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2881 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2941 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3001 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3061 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3121 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     3181 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3241 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     3301 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3361 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3421 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3481 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3541 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3601 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3661 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3721 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3781 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3841 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3901 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3961 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4021 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4081 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4141 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4201 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4261 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4321 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4381 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4441 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4501 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4561 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4621 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4681 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4741 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4801 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4861 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4921 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4981 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5041 gg
//
