LOCUS       sequence-36367-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2_F1-GTT
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..39
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             40..105
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggatctgcca ccatggctcc aaagaagaag cgtaaggtag actacaaaga ccatgacggt
       61 gattataaag atcatgacat cgattacaag gatgacgatg acaagggtac caagccctat
      121 aaatgtcctg aatgtggcaa gtccttctcg acctctggca acctcacccg tcaccaacgt
      181 acgcacaccg gcgaaaaacc atatgcttgc cctgtcgagt cctgcgatcg ccgcttttct
      241 tatcggcagt ctcttacccg gcatatccgg attcacacag gccagaagcc cttccagtgt
      301 cgcatctgca tgaggaactt cagccgctct gacaacctca cccgccacat ccgcacccac
      361 acaggccgag aagccttttg catgcgacat ctgcggcagg aagttccgtc agagtagtca
      421 tctcaagcag cacaccaaga tccacacagg tggatcccaa ctagtcaaaa gtgaactgga
      481 ggagaagaaa tctgaacttc gtcataaatt gaaatatgtg cctcatgaat atattgaatt
      541 aattgaaatt gccagaaatt ccactcagga tagaattctt gaaatgaagg taatggaatt
      601 ttttatgaaa gtttatggat atagaggtaa acatttgggt ggatcaagga aaccggacgg
      661 agcaatttat actgtcggat ctcctattga ttacggtgtg atcgtggata ctaaagctta
      721 tagcggaggt tataatctgc caattg
//
