LOCUS       sequence-363841-       11697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deletion of BHLH domain of mouse TFAP4 in combination with 
            TFAP4_BHLH_3-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Schaefer M, Nabih A, Spies D, Hermes V, Bodak M, Wischnewski H, 
            Stalder P, Ngondo RP, Liechti LA, Sajic T, Aebersold R, Gatfield D, 
            Ciaudo C
  TITLE     Global and precise identification of functional miRNA targets in 
            mESCs by integrative analysis.
  JOURNAL   EMBO Rep. 2022 Jul 28:e54762. doi: 10.15252/embr.202254762.
  PUBMED    35899551
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Jul 28:e54762. doi: 10.15252/embr.202254762."
COMMENT     Sequence Label: TFAP4_BHLH_5-1
FEATURES             Location/Qualifiers
     source          1..11697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(183..202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(184..202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(212..229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(212..228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(221..243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      370..825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(457..476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     667..688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        851..955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(956..1024,1025..1816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1174..1193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1987..2575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2476..2495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2729..2746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2863..2884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2899..2904,2905..2922,2923..2929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2937..2953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2942..2964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2961..2977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2961..2977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2996..3016
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2998..3016
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3044..3270
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3271..3451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3498..3623
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4116..4349
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4534..4578
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4756..5004
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4756..4776
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4927..4946
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5026..5101
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5149..5266
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5335..5546
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5566..5575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5575..5598
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5605..5625
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5650..9750
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5650..9750
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9751..9798
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9805..9861
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(9862..9864,9865..10578)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9862..9864,9865..10575)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(10165..10184)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10512..10533
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10593..11181
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10646..10666)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11064..11075)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11253..11486
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11564..11685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11601..11620)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11655..11674
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
       61 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
      181 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
      241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      301 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      361 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      421 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      481 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      541 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      601 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      661 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      721 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      781 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
      841 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      901 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      961 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1021 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1081 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1141 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1201 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1261 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1321 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1381 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1441 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1501 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1561 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1621 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1681 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1741 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1801 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1861 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1921 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1981 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2041 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2101 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2161 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2221 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2281 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2341 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2401 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2461 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2521 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2581 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2641 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2701 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2761 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     2821 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     2881 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     2941 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     3001 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     3061 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     3121 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     3181 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     3241 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     3301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3421 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     3481 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     3541 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     3601 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     3661 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     3721 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     3781 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     3841 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     3901 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     3961 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     4021 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     4081 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     4141 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     4201 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     4261 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     4321 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     4381 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     4441 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     4501 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     4561 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     4621 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     4681 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     4741 ccgaggggac ccagagaggg cctatttccc atgattcctt catatttgca tatacgatac
     4801 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
     4861 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     4921 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     4981 atcttgtgga aaggacgaaa caccggggtc agtggaatgt tggctgtttt agagctagaa
     5041 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     5101 cttttttgaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     5161 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     5221 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     5281 ggacagcaga gatccacttt ggcgccggct cgagtggctc cggtgcccgt cagtgggcag
     5341 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     5401 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     5461 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     5521 gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg gatccgccac catggattac
     5581 aaagacgatg acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc
     5641 ccagcagccg acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg
     5701 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
     5761 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
     5821 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
     5881 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
     5941 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
     6001 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
     6061 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
     6121 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
     6181 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
     6241 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
     6301 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
     6361 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
     6421 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
     6481 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
     6541 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
     6601 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
     6661 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
     6721 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
     6781 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
     6841 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
     6901 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
     6961 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
     7021 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
     7081 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
     7141 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
     7201 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
     7261 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
     7321 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
     7381 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
     7441 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
     7501 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
     7561 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
     7621 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
     7681 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
     7741 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
     7801 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
     7861 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
     7921 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
     7981 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
     8041 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
     8101 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
     8161 gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
     8221 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
     8281 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
     8341 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
     8401 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
     8461 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
     8521 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
     8581 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
     8641 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
     8701 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
     8761 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
     8821 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
     8881 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
     8941 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
     9001 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
     9061 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
     9121 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
     9181 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
     9241 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
     9301 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
     9361 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
     9421 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
     9481 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
     9541 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
     9601 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
     9661 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
     9721 cggatcgacc tgtctcagct gggaggcgac aagcgtcctg ctgctactaa gaaagctggt
     9781 caagctaaga aaaagaaagc tagcgccacg aacttctctc tgttaaagca agcaggcgac
     9841 gtggaagaaa accccggtcc cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     9901 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     9961 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
    10021 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
    10081 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
    10141 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
    10201 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
    10261 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
    10321 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
    10381 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
    10441 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
    10501 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
    10561 gacgagctgt acaagtaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
    10621 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
    10681 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
    10741 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
    10801 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
    10861 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
    10921 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
    10981 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
    11041 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
    11101 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
    11161 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
    11221 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
    11281 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
    11341 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    11401 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    11461 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
    11521 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
    11581 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11641 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggct
//
