LOCUS       sequence-363977-       10795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible fish Eng2b (mut) activation in zebrafish 
            permanent transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Amblard I, Thauvin M, Rampon C, Queguiner I, Pak VV, Belousov V, 
            Prochiantz A, Volovitch M, Joliot A, Vriz S
  TITLE     H2O2 and Engrailed 2 paracrine activity synergize to shape the 
            zebrafish optic tectum.
  JOURNAL   Commun Biol. 2020 Sep 29;3(1):536. doi: 10.1038/s42003-020-01268-7.
  PUBMED    32994473
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-09-29"; volume: "3(1):536. doi"; pages: " 
            10.1038/s42003-020-01268-7"
COMMENT     Sequence Label: pT22Ubm4Eng2bKRPC(#493)
FEATURES             Location/Qualifiers
     source          1..10795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(152..172)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(152..170)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(181..192,193..380)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    399..520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(540..1247,1248..1250,1251..1253))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(579..600)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     928..947
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1187..1208
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2014..2025)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5677..5706
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5716..5745
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5755..5784
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5794..5823
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6652..6672
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7639..7695
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7705..8415
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8443..8564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8660..8797,8798..8809)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8877..8894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9048..9636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9128..9147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9807..10598,10599..10667))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10430..10449
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10668..10772)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc ccgatactcc agcttttgtt ccctttagtg agggttaatt gcgcgttaca
      181 cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg
      241 ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca
      301 tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac
      361 ttattttttg gagatcactt cctcaggacc agacatgata agatacattg atgagtttgg
      421 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      481 tgctttattt gtaaccatta taagctgcaa taaacaagtt gcgtgcgcat ttatttaagg
      541 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      601 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      661 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      721 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat gccgttcttc
      781 tgcttgtcgg cggtgatata gacgttgtgg ctgatgtagt tgtactccag cttgtgcccc
      841 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      901 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      961 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     1021 tggtcggggt agcggctgaa gcactgcacg ccccaggtca gggtggtcac gagggtgggc
     1081 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     1141 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     1201 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catgtcggct
     1261 ggcgaccggt ggatcatccc ccgcaagtct gactgagtgg aagtttcagt tcaatggaag
     1321 ggagaaaaga gccagccttt atatatgtca tgtattgctg ctgtctgcat gcgatgaacc
     1381 tagagcttac tttttctgtc aagtaaggca aaagcagaga acctttgtct tgcattgtgt
     1441 ttgatgttgt gctgtgacag tcatttctta gttcatgctg gtacttttgt tccatagctt
     1501 tacccaaaga gttatccagc attcccagtt attccaaaaa ctcaatgtca caaattttcc
     1561 agtccattgc ttagcagcgt tgaccccgga gaccctgatg tggtaattta cagtgcaatt
     1621 cagttgctgg tccaaacact ttgtaacagt ttaaagaatt gtggttacta gtaaatcctt
     1681 aatttggcat catctaggtg tcattctttg ccacttttga atcacacaaa tgaaccaaac
     1741 aatggttagc tagcatcaaa catttaccaa ataatctcat cagtgtcacc ccaatgttta
     1801 agaagccaca ttttatgtcc ccagatatat taatattatt ttatatataa tttaaaatag
     1861 taatgtggtg ttactgtatt tgtcatataa cgcaatatat acagtatgtc tttatctgta
     1921 tcaattttgt ggtcacacca tcaataaact ccagtttaat tatttaaaat tgggtagtga
     1981 taaccatata aaggaacagt ggctagttcc atgttgtagc tcccaacctg ctttcaggtg
     2041 atccatgcag tgtcgtataa aaaggtaaca atttttaacc ttggaaagca agtacactta
     2101 aggtcccaga tctgaagcta cccctaacac actattctac acacagaaaa tgctcttcac
     2161 taggtcgaga ccagcaaagt tctagaattt gtcgaaacat ttatgttata tatttcctga
     2221 aaaaaattct gagtaagttc ttaagtgtta ttgccagcaa cataaacaac agacggcaaa
     2281 atgaataaat gataacaaag cagtaggctt aaataaacct aatttttata ggctgttctc
     2341 tacaaccctc aaacagtgat tagttttgta cttataaact tgccctttca ttcatatttc
     2401 aagaaaattg gttcagaaga tctggatatt ctagcagttg ttcaagctca tggagggatc
     2461 agtgacctga ttccacaatg actaggccta atccagaaat tagatgactg tcaacataaa
     2521 aaggcacagc actcactagc tgccctatat attttattat attttacata tattatttta
     2581 tttatttagc tctgagtgct gtactttctg gttaaagaaa actgcttaca acagctaacc
     2641 tgtactacct caggctcagg gaatttggaa caggtttgtc tggtttgttt ctttaaccat
     2701 gcatgcttgt tttcaactat ggcaacacag tcacatggga cattacagaa atgatttgtc
     2761 gatgacatgc gacttttctt taataaagcg caaagatccc aaaaagcaaa cttttaacaa
     2821 aaatcatata attatatttt caatccagct ttgtagcaac tttgtgctgc tgttcactca
     2881 gcaacagata gtcagtataa ggtcagtgtg tctcaaagca gtgccatctg tttcacacat
     2941 tgcgttctat atataagtgt gctggttgac acgacactgt ataaggccta ggctaaaaca
     3001 caaacaatgt agaatgacac tgtgtttttt ttgtaaacaa atgttgtttt tggttaaaca
     3061 tctttgtgaa aacatcctcc tgtcatgtat ttgctatatt caaatgttaa acccgtgcag
     3121 aatagaacat atacaaaaaa aaacaacaca acacattttt aaacattatt aaatatcaag
     3181 tattgctggc agttctgttt ctgttttaca gtaccctttg ccacagttct ccgcttttcc
     3241 tggtccagat tccacaagtc tgattcacca atagcaaagc gaataaacaa ccaaagcagc
     3301 caatcactgc ttgtagactg tcctgcgaga ccggcccatt ccagcacatt ctggaaactt
     3361 cctttatatg ataattataa atacatttaa attattgata caaaacatgt aattcctaga
     3421 acataaccat agcaatcatt agttttcagg gtaattatgt atttttagga tttgactgcg
     3481 gaaagatctg gtcatgtgac gtctcatgaa cgtcacggcc ctgggtttct ataaatacag
     3541 taggactctc gaccatcggc agatttttcg aagaagaaga tcagtttcag gagccgtact
     3601 gttccgtttc aacgcaaata ttaacggtaa gagcgaattt cctagtttgt tttcatgcca
     3661 ttctttaaaa ccatagcgta ttactttaat tatagtaaac tttcgctttc tttattacaa
     3721 gagacgtttt gtgttgattc tccgcggaca ttttcggtca gacaatcaga aaatgaccgc
     3781 ggaggaccag taacttgcat tacacgtaag ttaaatcttc gtgtattaaa atggttaggt
     3841 tgttaacgtc aaataggtta ccgtgtttgc gtgtgatcag gttggttttg ttagattttt
     3901 gtcagtattt ttaatttatt tgttttagtt tatttatttt ttttgctgaa tcatagtttg
     3961 tgaacaaaga acccggatgt tacatacagt acagccgcca tgttacagag agttataact
     4021 taatcatttt aaaaataatt ttgccttact tttagtttgt catgttgaga aatgaggaaa
     4081 tgttaaaatg aggaaatatc caattaattt aatatatcaa aataatccat gattacaatg
     4141 cactgaactg gagaaaatta agatgttttc tagtgtcatg aaacaaatgt aagagatgta
     4201 cattgtagat gttttatgtc aagaattggc tagttgatgc agcatactgg cgatactcag
     4261 ttgtaataac agtaacgtta catgttaata gactactgag tatgctgttc tgtctatgta
     4321 tgctctgtaa gctacgagaa ggactttttt aaacagtaaa gggtgcaata tttttacaaa
     4381 ttgaattaaa taaaggctgt ctattaagta atatgcttga tatttttctt acttgatcgg
     4441 aaataagaaa aaatataaac gttgttgctc taaaaatcct agttcagttt agccaaccac
     4501 aaataccttt ttgttcctcc aacagttttt ttttcttctc tataatattt ggcagtctat
     4561 agtactccaa atgtttcccc acagtctaac taattggtac agccaaaatc atgacactta
     4621 ttgcaataat aattttggtt cattggcatt gttgatagcc tgtgccacta atatggtcga
     4681 ttgatcatgc ttcaggaaga aaactatatt gtttgatgta agattattaa atcttcacct
     4741 gcttccatta caaactattc ccatcttatt gaattctggt atgtcttaaa ggattagttc
     4801 acttcccaaa tcaaaattta cttagccttt tttcatccat gatccctttt tttcatcatt
     4861 aatgaagaaa ttgtttttga aaaagtttca agattttttt ctctatattg tggagcttgt
     4921 tagtttaaaa ttccaaaatg caatatgtgg cttcaaatgg ttctaaatga tcccagtcaa
     4981 ggaataacag tcttatctaa tgaaaccatt agacctttaa aaataaaaaa aataaaagta
     5041 tttattttta aatgactgag tgattaagtt gaatttcagc gtttccttac tgtgtagaag
     5101 tccttcctta ctggccccac cctttggttc tctgccaatc tgctacctaa tgtaatgttg
     5161 tggaacatta ttattcttta tttcttaatt ttttattttt tattttaaaa caatgtaaac
     5221 tgcacagatg tgcagtttgt ttaaaatggc caatgctttg gaaatgcatg acataattag
     5281 atttcatgat gcacaaagcc aaatctcaga gcttgtgcaa aatgagctat catttcacta
     5341 ggtaagaccc taaattttca tataggatca tttggacaat tttgctgcag gtaaaatgca
     5401 ttctatagtc cactgtcagc cattgttttg gatagtattt atttttctct acaagtatag
     5461 tcaatagttt tctattattt taaaggtttg taacatttaa gggtgaccaa atgcaaagta
     5521 aaatttcatt ttcgggtgaa ctatctcgtt taacatggga gaagtgcaaa acatacatta
     5581 ttggctagaa cattgtagta ttttttaaat ggaaatgtgt gattgctaat cttactttga
     5641 atttgtttac agggatccca tcgatttaaa gctatggagc aaaagctcat ttctgaagag
     5701 gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa
     5761 aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac
     5821 ttgaatgaaa tggagagctt gggcgacctc accatggaag aaaacgatca tagcaacaga
     5881 gatgtggagc gccaggattc gggcgacgag tccaataggg ctattcttcc tctgttgcag
     5941 gctcctggaa acgtactccc tcacagaatc accaactttt acatcgacaa tattttaaga
     6001 ccagattttg gccgcaggaa agaaggaagc agacgcgacg aaattaacat agttgagaga
     6061 gagaaccgct gtccatcggc ccccggatca ggacaggtag ctccagtatc gggggaagga
     6121 acatcgagcc ctcgcgctgt aaacgcatct aaaaaaactg atattagcac ggacgaatct
     6181 ctgaaatccc gtgcagagac tggagatcag tgtttaagtt ccgattcgga ctgctcacaa
     6241 agatgcgctg cccaggcgaa acagcctatg ctgaagcccg ccaaggtata ctgcacgcgt
     6301 tattcagaca gaccttcatc tggaccaaga tcacgcaaac caaagaagaa aaccccaaca
     6361 aaggaagaca agagaccgag aacagcattc acagcggagc aactacagag actcaagaat
     6421 gagttccaga ataatcgtta cctgacggag caaaggagac aagccctggc ccaggaactc
     6481 ggcctgaacg agtctcagat caaaatctgg tttcaaaaca aaagggcaaa gatcaaaaaa
     6541 gcaacgggga acaaaaacac acttgccgtg cacctgatgg cacagggact ttacaatcac
     6601 gccacagtaa caaaggacga caaatcagac agtgatggac tgcccgggcc agagaacctg
     6661 tacttccagg ggctcgagcc ctctgccgga gacatgagag ctgcaaacct ttggccgagc
     6721 ccgctgatga ttaaacgctc taagaagaac agcctggcct tgtccctgac cgccgaccag
     6781 atggtcagtg ccttgttgga tgccgagccc cccatactct attccgagta tgatcctacc
     6841 agacccttca gtgaggcttc gatgatgggc ttactgacca acctggcaga cagggagctg
     6901 gttcacatga ttaactgggc caagagggtg ccaggctttg tggatttgac cctccacgat
     6961 caggtccacc tcctagaatg tgcctggcta gagatcctga tgattggtct cgtctggcgc
     7021 agcatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga caggaaccag
     7081 ggaaaatgtg tagagggcat ggtggagatt ttcgacatgc tgctggctac atcatctcgg
     7141 ttccgcatga tgaatctgca gggagaggaa tttgtgtgcc tcaaatctat tattttgctt
     7201 aattctggag tttacacatt tctgtccagc accctgaagt ctctggagga aaaggaccat
     7261 atccatcgag tgctggacaa gatcacagac actttgattc acctgatggc taaggctggc
     7321 ctgaccctgc agcagcagca tcagagactc gcacagcttc tcctgatcct ctcccacatc
     7381 aggcacatga gtaacaaagg catggagcat ctgtactcta tgaagtgcaa gaacgtggtg
     7441 cccctctatg atctgctgct ggaggcggcg gacgctcacc gcctacatgc gcccactagc
     7501 cgtggagggg catccgtgga ggaaacagac caaagccact tggctactgc gggctctact
     7561 tcatctcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccaca
     7621 gcaccaggtt ccggcagcgc tacaaacttc tctctgctca agcaggctgg agatgtggaa
     7681 gaaaaccctg ggcccgaaga cggcatggtg agcaagggcg aggaggataa catggctatc
     7741 atcaaggagt tcatgcgctt caaggttcac atggagggct ccgtgaacgg ccacgagttc
     7801 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     7861 gtgacaaagg gcggccccct gcccttcgcc tgggacatcc tgagccctca gttcatgtac
     7921 ggctccaaag cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtctttc
     7981 ccagagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgacagtg
     8041 acccaggact cttctctgca ggacggcgag tttatctaca aggtgaagct gcgcggcacc
     8101 aacttcccct ccgacggccc cgtaatgcag aagaaaacta tgggatggga ggcttcttcc
     8161 gagcggatgt acccagagga cggagccctg aagggcgaga tcaagcagag gctgaagctg
     8221 aaggacggcg gccactacga tgccgaggtc aagaccacct acaaggccaa gaagcccgtg
     8281 caactgcccg gagcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     8341 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc acagcaccgg cggcatggac
     8401 gagctgtaca agtaaggcgc gccgatatct acgtagacat gataagatac attgatgagt
     8461 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     8521 ctattgcttt atttgtaacc atattaagct gcaataaaca agttaacaac aacaattgca
     8581 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcctgc aggactctag
     8641 tcgcggccgc tcttccgcta atactcaagt acaattttaa tggagtactt ttttactttt
     8701 actcaagtaa gattctagcc agatactttt acttttaatt gagtaaaatt ttccctaagt
     8761 acttgtactt tcacttgagt aaaatttttg agtacttttt acacctctgc cctgcattaa
     8821 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8881 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8941 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9001 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9061 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9661 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9721 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9781 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9841 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9901 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9961 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10021 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10081 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10141 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10201 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10261 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10321 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10381 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10441 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10501 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10561 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10621 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10681 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10741 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
