LOCUS       sequence-364017-       10066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cyclofen-inducible apoptosis activation and imaging (tEosFP) in 
            zebrafish transient transgenic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Feng Z, Zhang W, Xu J, Gauron C, Ducos B, Vriz S, Volovitch M, 
            Jullien L, Weiss S, Bensimon D
  TITLE     Optical control and study of biological processes at the single-cell
            level in a live organism.
  JOURNAL   Rep Prog Phys. 2013 Jul;76(7):072601. doi: 
            10.1088/0034-4885/76/7/072601. Epub 2013 Jun 14.
  PUBMED    23764902
REFERENCE   2  (bases 1 to 10066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rep Prog 
            Phys."; date: "2013-07"; volume: "76(7):072601. doi"; pages: " 
            10.1088/0034-4885/76/7/072601. Epub 2013 Jun 14"
COMMENT     Sequence Label: pT2iApowriter2(#336)
FEATURES             Location/Qualifiers
     source          1..10066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(150..170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(150..168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(179..190,191..378)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     CDS             3977..4018
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             4499..4528
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4538..4567
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4577..4606
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4616..4645
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4655..4684
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4715..5395
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIA
                     RNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     CDS             5423..5479
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6863..6883
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    7856..7977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7867..7886)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7921..7940
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8096..8233,8234..8245)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(8317..8905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8397..8416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9076..9867,9868..9936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9699..9718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9937..10041)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccc gatactccag cttttgttcc ctttagtgag ggttaattgc gcgttacaca
      181 gaggtgtaaa gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg
      241 atttttactt tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt
      301 tttttagaaa aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt
      361 attttttgga gatcacttcc tcatagggat aacagggtaa tggccggcct tttttaggtc
      421 ccagatctga agctacccct aacacactat tctacacaca gaaaatgctc ttcactaggt
      481 cgagaccagc aaagttctag aatttgtcga aacatttatg ttatatattt cctgaaaaaa
      541 attctgagta agttcttaag tgttattgcc agcaacataa acaacagacg gcaaaatgaa
      601 taaatgataa caaagcagta ggcttaaata aacctaattt ttataggctg ttctctacaa
      661 ccctcaaaca gtgattagtt ttgtacttat aaacttgccc tttcattcat atttcaagaa
      721 aattggttca gaagatctgg atattctagc agttgttcaa gctcatggag ggatcagtga
      781 cctgattcca caatgactag gcctaatcca gaaattagat gactgtcaac ataaaaaggc
      841 acagcactca ctagctgccc tatatatttt attatatttt acatatatta ttttatttat
      901 ttagctctga gtgctgtact ttctggttaa agaaaactgc ttacaacagc taacctgtac
      961 tacctcaggc tcagggaatt tggaacaggt ttgtctggtt tgtttcttta accatgcatg
     1021 cttgttttca actatggcaa cacagtcaca tgggacatta cagaaatgat ttgtcgatga
     1081 catgcgactt ttctttaata aagcgcaaag atcccaaaaa gcaaactttt aacaaaaatc
     1141 atataattat attttcaatc cagctttgta gcaactttgt gctgctgttc actcagcaac
     1201 agatagtcag tataaggtca gtgtgtctca aagcagtgcc atctgtttca cacattgcgt
     1261 tctatatata agtgtgctgg ttgacacgac actgtataag gcctaggcta aaacacaaac
     1321 aatgtagaat gacactgtgt tttttttgta aacaaatgtt gtttttggtt aaacatcttt
     1381 gtgaaaacat cctcctgtca tgtatttgct atattcaaat gttaaacccg tgcagaatag
     1441 aacatataca aaaaaaaaca acacaacaca tttttaaaca ttattaaata tcaagtattg
     1501 ctggcagttc tgtttctgtt ttacagtacc ctttgccaca gttctccgct tttcctggtc
     1561 cagattccac aagtctgatt caccaatagc aaagcgaata aacaaccaaa gcagccaatc
     1621 actgcttgta gactgtcctg cgagaccggc ccattccagc acattctgga aacttccttt
     1681 atatgataat tataaataca tttaaattat tgatacaaaa catgtaattc ctagaacata
     1741 accatagcaa tcattagttt tcagggtaat tatgtatttt taggatttga ctgcggaaag
     1801 atctggtcat gtgacgtctc atgaacgtca cggccctggg tttctataaa tacagtagga
     1861 ctctcgacca tcggcagatt tttcgaagaa gaagatcagt ttcaggagcc gtactgttcc
     1921 gtttcaacgc aaatattaac ggtaagagcg aatttcctag tttgttttca tgccattctt
     1981 taaaaccata gcgtattact ttaattatag taaactttcg ctttctttat tacaagagac
     2041 gttttgtgtt gattctccgc ggacattttc ggtcagacaa tcagaaaatg accgcggagg
     2101 accagtaact tgcattacac gtaagttaaa tcttcgtgta ttaaaatggt taggttgtta
     2161 acgtcaaata ggttaccgtg tttgcgtgtg atcaggttgg ttttgttaga tttttgtcag
     2221 tatttttaat ttatttgttt tagtttattt attttttttg ctgaatcata gtttgtgaac
     2281 aaagaacccg gatgttacat acagtacagc cgccatgtta cagagagtta taacttaatc
     2341 attttaaaaa taattttgcc ttacttttag tttgtcatgt tgagaaatga ggaaatgtta
     2401 aaatgaggaa atatccaatt aatttaatat atcaaaataa tccatgatta caatgcactg
     2461 aactggagaa aattaagatg ttttctagtg tcatgaaaca aatgtaagag atgtacattg
     2521 tagatgtttt atgtcaagaa ttggctagtt gatgcagcat actggcgata ctcagttgta
     2581 ataacagtaa cgttacatgt taatagacta ctgagtatgc tgttctgtct atgtatgctc
     2641 tgtaagctac gagaaggact tttttaaaca gtaaagggtg caatattttt acaaattgaa
     2701 ttaaataaag gctgtctatt aagtaatatg cttgatattt ttcttacttg atcggaaata
     2761 agaaaaaata taaacgttgt tgctctaaaa atcctagttc agtttagcca accacaaata
     2821 cctttttgtt cctccaacag tttttttttc ttctctataa tatttggcag tctatagtac
     2881 tccaaatgtt tccccacagt ctaactaatt ggtacagcca aaatcatgac acttattgca
     2941 ataataattt tggttcattg gcattgttga tagcctgtgc cactaatatg gtcgattgat
     3001 catgcttcag gaagaaaact atattgtttg atgtaagatt attaaatctt cacctgcttc
     3061 cattacaaac tattcccatc ttattgaatt ctggtatgtc ttaaaggatt agttcacttc
     3121 ccaaatcaaa atttacttag ccttttttca tccatgatcc ctttttttca tcattaatga
     3181 agaaattgtt tttgaaaaag tttcaagatt tttttctcta tattgtggag cttgttagtt
     3241 taaaattcca aaatgcaata tgtggcttca aatggttcta aatgatccca gtcaaggaat
     3301 aacagtctta tctaatgaaa ccattagacc tttaaaaata aaaaaaataa aagtatttat
     3361 ttttaaatga ctgagtgatt aagttgaatt tcagcgtttc cttactgtgt agaagtcctt
     3421 ccttactggc cccacccttt ggttctctgc caatctgcta cctaatgtaa tgttgtggaa
     3481 cattattatt ctttatttct taatttttta ttttttattt taaaacaatg taaactgcac
     3541 agatgtgcag tttgtttaaa atggccaatg ctttggaaat gcatgacata attagatttc
     3601 atgatgcaca aagccaaatc tcagagcttg tgcaaaatga gctatcattt cactaggtaa
     3661 gaccctaaat tttcatatag gatcatttgg acaattttgc tgcaggtaaa atgcattcta
     3721 tagtccactg tcagccattg ttttggatag tatttatttt tctctacaag tatagtcaat
     3781 agttttctat tattttaaag gtttgtaaca tttaagggtg accaaatgca aagtaaaatt
     3841 tcattttcgg gtgaactatc tcgtttaaca tgggagaagt gcaaaacata cattattggc
     3901 tagaacattg tagtattttt taaatggaaa tgtgtgattg ctaatcttac tttgaatttg
     3961 tttacaggga tccatgatgg ggagtagcaa gagcaagcct aaagacccta gccagagaag
     4021 caggggatct accggtgcat ctgttgtagc tgatcttccg tcttatggac ctatcgcttt
     4081 cgatcaggtg gatggcgtca cgaacgcgac ccagctattc aaaaataata taaacaaaac
     4141 cagaatgaac gatttaaacc gcgaggagac acgattaaag accttcaccg actggccgct
     4201 agattggctg gataaacgcc agctggccca aaccggcatg tacttcacac acgctggcga
     4261 caaagttaaa tgctttttct gcggcgtgga aatcggttgc tgggagcagg aggatcagcc
     4321 cgtgccggaa catcagcgat ggtcgcccaa ctgtccactg ttgcgcagga gaactaccaa
     4381 caatgtgccg atcaatgccg aagcattaga tcgcatcctg ccgccaataa gctacgacat
     4441 ctgcggcgcc aacgactcga cgctagagat gaggagatcc catcgattta aagctatgga
     4501 gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca tttctgaaga
     4561 ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg aaatggagca
     4621 aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt ctgaagagga
     4681 cttgaatgaa atggagagct tgggcgacct caccatggtg agtgcgatta agccagacat
     4741 gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cattttgtga tcgacggaga
     4801 tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg
     4861 acctctgcct tttgcctttg acatcctgac taccgctttc cattacggca acagggtatt
     4921 cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta aaggatattc
     4981 gtgggaacgc agcttgactt tcgaggacgg aggcatttgt atcgccagaa acgacataac
     5041 aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact ttcccgccaa
     5101 tggtccagtt atgcagaaga aaacgctgaa atgggagccc tccactgaga aaatgtatgt
     5161 gcgtgatgga gtgctgacgg gtgatattac aatggctttg ttgcttgaag gaaatgccca
     5221 ttaccgatgt gacttcagaa ctacttacaa agctaaagag aagggtgtca agttaccagg
     5281 ctaccacttt gttgaccact gtattgagat tttaagccat gacaaagatt acaacaaggt
     5341 taagctgtat gagcacgctg ttgctcattc tggattgcct gacaatgcca ggagatctcc
     5401 caagaagagg aaggtgagat ctgccaccaa ttttagcctc ctgaaacaag caggcgacgt
     5461 tgaggagaat ccaggaccgt ctcccatgat ggacgaggcg gaccgccagc tcctgagaag
     5521 atgcagggtg cgcctagtga gcgagctgca agtcgcggag ctgtgggacg ctctgctgag
     5581 tcgagagctg ttcacgcgcg acatgatcga ggatattcag caggcaggat ctggctctcg
     5641 gcgggatcag gctaggcagc tcgtcacaga cctggagact agaggccgcc aggctctgcc
     5701 tctgttcatc tcctgcttag aggacacagg ccaaggcacc ctggcttcac tcttgcaaag
     5761 cggtcggcaa gcagccaagc aagacccaga ggctgttaaa cccctagacc atctggtgcc
     5821 tgtggtcctg ggaccaatgg gactcacagc aaaggagcag agagtagtga agctggaccc
     5881 gtcacagcct gccgtgggaa acctgacccc agtggtgctc ggacctgaag aactctggcc
     5941 tgctcgcctg aagccagagg ttctcagacc agaaacacct agacccgtgg acattggttc
     6001 tggtggagcc catgatgtct gtgttccagg gaagatcagg ggacatgcag atatggcata
     6061 caccctggac tctgatccct gcggccactg tctcatcatc aacaatgtga acttctgccc
     6121 ttcctcgggg ctcggcacac gcacgggctc caacttggac cgtgacaaac tggagcaccg
     6181 attccgctgg ctgcgcttca tggtggaggt gaagaacgac ctgactgcta agaaaatggt
     6241 cacggctttg atggagatgg cacaccggaa ccaccgtgcc ctggactgct ttgtggtggt
     6301 catcctctct catggctgcc aggccagcca cctccagttc ccaggtgctg tttatgggac
     6361 agatggatgc tccgtgtcca ttgagaaaat tgtgaacatc tttaacggaa gcggctgccc
     6421 tagcctggga gggaagccca aactgttctt catccaggct tgcggaggtg agcagaaaga
     6481 ccacggcttt gaggtggctt gcacttcctc tcagggcaga accttggaca gtgactctga
     6541 gcctgatgct gtcccctatc aggaaggccc aagacccttg gaccaactgg atgctgtgtc
     6601 aagtttgcct acccctagcg acatccttgt gtcctactcc accttcccag gatttgtgtc
     6661 ttggagggac aagaaaagtg gctcctggta catcgagact ttggacggta ttctggaaca
     6721 gtgggctcgc tctgaggatc tgcagtccct ccttctcagg gttgccaatg ctgtttctgc
     6781 gaaagggact tacaagcaga ttcctggctg ttttaacttc ctgcggaaaa agctgttttt
     6841 caaaacttct gtgcccgggc cagagaacct gtacttccag gggctcgagc cctctgccgg
     6901 agacatgaga gctgcaaacc tttggccgag cccgctgatg attaaacgct ctaagaagaa
     6961 cagcctggcc ttgtccctga ccgccgacca gatggtcagt gccttgttgg atgccgagcc
     7021 ccccatactc tattccgagt atgatcctac cagacccttc agtgaggctt cgatgatggg
     7081 cttactgacc aacctggcag acagggagct ggttcacatg attaactggg ccaagagggt
     7141 gccaggcttt gtggatttga ccctccacga tcaggtccac ctcctagaat gtgcctggct
     7201 agagatcctg atgattggtc tcgtctggcg cagcatggag cacccagtga agctactgtt
     7261 tgctcctaac ttgctcttgg acaggaacca gggaaaatgt gtagagggca tggtggagat
     7321 tttcgacatg ctgctggcta catcatctcg gttccgcatg atgaatctgc agggagagga
     7381 atttgtgtgc ctcaaatcta ttattttgct taattctgga gtttacacat ttctgtccag
     7441 caccctgaag tctctggagg aaaaggacca tatccatcga gtgctggaca agatcacaga
     7501 cactttgatt cacctgatgg ctaaggctgg cctgaccctg cagcagcagc atcagagact
     7561 cgcacagctt ctcctgatcc tctcccacat caggcacatg agtaacaaag gcatggagca
     7621 tctgtactct atgaagtgca agaacgtggt gcccctctat gatctgctgc tggaggcggc
     7681 ggacgctcac cgcctacatg cgcccactag ccgtggaggg gcatccgtgg aggaaacaga
     7741 ccaaagccac ttggctactg cgggctctac ttcatctcat tccttgcaaa agtattacat
     7801 cacgggggag gcagagggtt tccctgccac agcaccagga tctacgtaga catgataaga
     7861 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     7921 gaaatttgtg atgctattgc tttatttgta accatattaa gctgcaataa acaagttaac
     7981 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcc
     8041 tgcaggactc tagtcgcggc cctagggata acagggtaat ggccgctctt ccgctaatac
     8101 tcaagtacaa ttttaatgga gtactttttt acttttactc aagtaagatt ctagccagat
     8161 acttttactt ttaattgagt aaaattttcc ctaagtactt gtactttcac ttgagtaaaa
     8221 tttttgagta ctttttacac ctctgccctg catcagcatg tgagcaaaag gccagcaaaa
     8281 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8341 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8401 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8461 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8521 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8581 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8641 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8701 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8761 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8821 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8881 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8941 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9001 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9061 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9121 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9181 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9241 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9301 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9361 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9421 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9481 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9541 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9601 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9661 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9721 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9781 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9841 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9901 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9961 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10021 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacct
//
