LOCUS       sequence-364399-        8343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of gRNA scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, 
            Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG
  TITLE     Optimized libraries for CRISPR-Cas9 genetic screens with multiple 
            modalities.
  JOURNAL   Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8.
  PUBMED    30575746
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-21"; volume: "9(1):5416. doi"; pages: " 
            10.1038/s41467-018-07901-8"
COMMENT     Sequence Label: pXPR_050
FEATURES             Location/Qualifiers
     source          1..8343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    133..254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(144..163)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     198..217
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             332..565
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    637..1225
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             743..754
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1152..1172
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1241..1840)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1324..1344)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1821..1840
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1853..3031)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1862..2800
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(1885..1905)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3169..3286
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(3460..3708)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(3518..3537)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3688..3708)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(3886..3930)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4115..4348
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4841..4966
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5013..5193
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5194..5420)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5448..5468)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5448..5466)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5487..5503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5487..5503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5500..5522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5511..5527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5535..5541,5542..5559,5560..5565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5580..5601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5718..5735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5889..6477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5969..5988)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6648..7439,7440..7508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7271..7290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7509..7613)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7639..8094)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7776..7797)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7988..8007
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8221..8243
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8235..8252
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8236..8252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8262..8281
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8262..8280
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      join(8301..8343,1..93)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
       61 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggatagctag
      121 agccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      181 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      241 gcaataaaca agttcctctc actctctgat attcatttct ttgcaagtta taaatactga
      301 ataataagat gacatgaact actatacgta ctgctagaga ttttccacac tgactaaaag
      361 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
      421 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
      481 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
      541 cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
      601 acagctgcct tgtaagtcat tggtcttaaa gtcgacgcgg ggaggcggcc caaagggaga
      661 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
      721 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
      781 gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca
      841 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
      901 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
      961 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     1021 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     1081 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     1141 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     1201 aaattttgta atccagaggt tgattgtcga cttaacgcgt tcaggcaccg ggcttgcggg
     1261 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
     1321 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
     1381 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
     1441 ggtcgggcga gactccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
     1501 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
     1561 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
     1621 ccacggcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
     1681 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc
     1741 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt
     1801 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggtggccgta cgtcacgaca
     1861 cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc
     1921 caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt
     1981 cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta
     2041 aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact
     2101 ccgtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg
     2161 actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcagggc
     2221 cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc
     2281 agggcggggc gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag
     2341 actacccccg tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc
     2401 gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca
     2461 gatcttggcc cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc
     2521 aaaaatttta aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgccacc
     2581 agattcgcac gcggcggccc cagcgcccag gccaggcctc aactcaagca cgaggcgaag
     2641 gggctcctta agcgcaaggc ctcgaactct cccacccact tccaacccga agctcgggat
     2701 caagaatcac gtactgcagc caggtggaag taattcaagg cacgcaaggg ccataacccg
     2761 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc
     2821 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct
     2881 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc
     2941 tctaggcacc ggatcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg
     3001 tgcgctctgc ccactgacgg gcaccggagc caattcccac tcctttcaag acctagaagg
     3061 tccattagct gcaaagattc ctctctgttt aaaactttat ccatctttgc acccgggccc
     3121 cctgagccgg cgccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     3181 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     3241 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     3301 tgccatttgt ctcaagatct agttacgcca agcttgaatt caaaaaagca ccgactcggt
     3361 gccacttttt caagttgata acggactagc cttatttcaa cttgctacag catttctgct
     3421 gtagctctga aacagagacg caaaaaaccg gtcgtctccg gtgtttcgtc ctttccacaa
     3481 gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat
     3541 tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt
     3601 attttgtact aatatctttg tgtttacagt caaattaatt ctaattatct ctctaacagc
     3661 cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctctc tgggtcccct
     3721 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     3781 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     3841 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     3901 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     3961 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     4021 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     4081 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     4141 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     4201 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     4261 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     4321 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     4381 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     4441 atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg
     4501 ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt
     4561 ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct actgtattat
     4621 ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt
     4681 gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc
     4741 tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa
     4801 ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc
     4861 tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc
     4921 ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc
     4981 ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa
     5041 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     5101 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     5161 ccaggctcag atctggtcta accagagaga cccgtttatg tatcgagcta ggcacttaaa
     5221 tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca gacccgtctg
     5281 ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg
     5341 ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa
     5401 gagtattgca taagactaca ttaagcttgc agctccagct tttgttccct ttagtgaggg
     5461 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5521 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5581 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5641 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5701 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5761 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5821 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5881 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5941 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6001 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6061 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6121 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6181 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6241 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6301 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6361 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6421 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6481 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6541 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6601 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6661 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6721 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6781 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6841 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6901 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6961 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7021 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7081 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7141 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7201 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7261 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7321 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7381 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7441 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7501 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7561 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7621 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     7681 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     7741 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     7801 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     7861 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     7921 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     7981 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     8041 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     8101 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     8161 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     8221 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     8281 gcgaattggg tacgtcccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     8341 aga
//
