LOCUS       sequence-364416-        9671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses low-affinity split-TurboID C-terminal fragment in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Cho KF, Branon TC, Rajeev S, Svinkina T, Udeshi ND, Thoudam T, Kwak 
            C, Rhee HW, Lee IK, Carr SA, Ting AY
  TITLE     Split-TurboID enables contact-dependent proximity labeling in cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 May 18. pii: 1919528117. doi: 
            10.1073/pnas.1919528117.
  PUBMED    32424107
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 May 18. pii: 1919528117. doi: 
            10.1073/pnas.1919528117."
COMMENT     Sequence Label: pLX304 CMV HA-HaloTag-FRB-sTurboID (C)
FEATURES             Location/Qualifiers
     source          1..9671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1174..1762
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1227..1247)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1645..1656)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1834..2067
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2145..2266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2182..2201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2236..2255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2306..2441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2461..2480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2462..2480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2490..2507)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2490..2506)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2499..2521)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2648..3103
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2735..2754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2945..2966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3129..3233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3234..3302,3303..4094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4265..4853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4754..4773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5007..5024
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5141..5162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5177..5182,5183..5200,5201..5207)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5215..5231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5220..5242
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5239..5255
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5239..5255
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5274..5294
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5276..5294
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5322..5548
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5549..5729
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5776..5901
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6394..6627
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6812..6856
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7031..7531
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7450..7468
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7543..7938
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7998..8115
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8232..8535
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8536..8739
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8690..8710
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    8762..8786
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8807..8833
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagtgagat cgcgcgctgg ctctcgacgc tcgaaatttc cggcgctagc accaaaggtt
       61 ctggtgggac tggcgggagc gtggccatcc tgtggcacga gatgtggcat gaaggcctgg
      121 aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg
      181 agcccttgca tgctatgatg gaacggggcc cccagactct gaaggaaaca tcctttaatc
      241 aggcctatgg tcgagattta atggaggccc aagagtggtg caggaagtac atgaaatcag
      301 ggaatgtcaa ggacctcacc caagcctggg acctctatta tcatgtgttc cgacgaatct
      361 caggttccaa gggctcgggc tcgacctcgg gctcgggcgg acagctggac ggcgggagcg
      421 tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga atcggcgagc
      481 tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga agcagaggga
      541 ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg cgcctgaagc
      601 ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca gaagcgctgc
      661 gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat ctgcaggata
      721 gaaagctggc aggcatcctg gtggagctgg ccggaataac aggcgatgct gcacagatcg
      781 tcattggcgc cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg gtcaatcagg
      841 gctggatcac actgcaggaa gcagggatta acctggacag gaatactctg gccgctacgc
      901 tgatccgaga gctgcgggca gccctggaac tgttcgagca ggaaggcctg gctccatatc
      961 tgccacggtg ggagaagctg gataacttca tcaatagacc cgtgaagctg atcattgggg
     1021 acaaagagat tttcgggatt agccggggga ttgataaaca gggagccctg ctgctggaac
     1081 aggacggagt tatcaaaccc tggatgggcg gagaaatcag tctgcggtct gccgaaaagt
     1141 aagctagcgt aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     1201 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1261 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     1321 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     1381 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     1441 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     1501 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     1561 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     1621 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     1681 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     1741 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     1801 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     1861 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     1921 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     1981 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     2041 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     2101 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     2161 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2221 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     2281 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     2341 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     2401 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     2461 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     2521 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2581 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2641 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2701 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2761 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2821 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2881 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2941 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3001 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3061 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     3121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3241 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3301 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3361 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3421 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3481 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3541 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3601 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3661 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3721 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3781 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3841 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3901 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3961 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4021 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4081 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4141 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4381 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4621 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4921 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4981 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5041 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     5101 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     5161 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     5221 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     5281 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     5341 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     5401 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     5461 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     5521 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     5581 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5641 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5701 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     5761 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     5821 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     5881 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     5941 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     6001 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     6061 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     6121 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     6181 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     6241 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     6301 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     6361 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     6421 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     6481 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     6541 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     6601 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     6661 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     6721 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     6781 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     6841 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     6901 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     6961 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     7021 gaggggaccc ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     7081 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     7141 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     7201 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     7261 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     7321 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
     7381 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     7441 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     7501 cgttgaccga atcaccgacc tctctcccca gggggtacca ccatggccaa gcctttgtct
     7561 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     7621 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     7681 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     7741 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     7801 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     7861 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     7921 ggttatgtgt gggagggcct gcagctgcag tagtaagaat tctagatctt gagacaaatg
     7981 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     8041 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     8101 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc
     8161 gagggggccc ggggttatta atagtaatca attacggggt cattagttca tagcccatat
     8221 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     8281 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     8341 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     8401 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     8461 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     8521 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     8581 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     8641 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     8701 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tgtcgggatc
     8761 aacaagtttg tacaaaaaag caggcttcga aggagataga accatgtacc catacgacgt
     8821 tccagactac gcaatcggta caggcttccc cttcgacccc cattatgtgg aagtcctggg
     8881 cgagcgtatg cactacgtcg atgttggacc gcgggatggc acgcctgtgc tgttcctgca
     8941 cggtaacccg acctcgtcct acctgtggcg caacatcatc ccgcatgtag caccgagtca
     9001 tcggtgcatt gctccagacc tgatcgggat gggaaaatcg gacaaaccag acctcgatta
     9061 tttcttcgac gaccacgtcc gctacctcga tgccttcatc gaagccttgg gtttggaaga
     9121 ggtcgtcctg gtcatccacg actggggctc agctctcgga ttccactggg ccaagcgcaa
     9181 tccggaacgg gtcaaaggta ttgcatgtat ggaattcatc cggcctatcc cgacgtggga
     9241 cgaatggcca gaattcgccc gtgagacctt ccaggccttc cggaccgccg acgtcggccg
     9301 agagttgatc atcgatcaga acgctttcat cgagggtgcg ctcccgatgg gggtcgtccg
     9361 tccgcttacg gaggtcgaga tggaccacta tcgcgagccc ttcctcaagc ctgttgaccg
     9421 agagccactg tggcgattcc ccaacgagct gcccatcgcc ggtgagcccg cgaacatcgt
     9481 cgcgctcgtc gaggcataca tgaactggct gcaccagtca cctgtcccga agttgttgtt
     9541 ctggggcaca cccggcgtac tgatcccccc ggccgaagcc gcgagacttg ccgaaagcct
     9601 ccccaactgc aagacagtgg acatcggccc gggattgttc ttgctccagg aagacaaccc
     9661 ggaccttatc g
//
