LOCUS       sequence-364856-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CapChR1 in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Fernandez Lahore RG, Pampaloni NP, Peter E, Heim MM, Tillert L, 
            Vierock J, Oppermann J, Walther J, Schmitz D, Owald D, Plested AJR, 
            Rost BR, Hegemann P
  TITLE     Calcium-permeable channelrhodopsins for the photocontrol of calcium 
            signalling.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4.
  PUBMED    36543773
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7844. doi"; pages: " 
            10.1038/s41467-022-35373-4"
COMMENT     Sequence Label: CapChR1_pUAST-attb
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7..933
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFDILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKECWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
ORIGIN
        1 gccaccatgg actatggcgg cgctttgtct gccgtcggac gcgaactttt gttcgttact
       61 aatcctgtgg tggtgaacgg gtccgtcctg gtccctgagg atcaatgtta ctgtgccgga
      121 tggattgaat ctcgcggcac gaacggcgct cagaccgcgt caaatgtcct gcagtggctt
      181 gcagcaggat tcgacatttt gctgctgatg ttctatgcct accaaacctg gaaatctaca
      241 tgcggctggg aggagatcta tgtgtgcgcc attgaaatgg ttaaggtgat tctcgagttc
      301 ttttttgagt ttaagaatcc ctctatgctc taccttgcca caggacaccg ggtgcagtgg
      361 ctgcgctatg cagagtggct gctcacttgt cctgtcatct gtatccacct gagcaacctc
      421 accggcctga gcaacgacta cagcaggaga accatgggac tccttgtctc agacatcggg
      481 tgtatcgtgt ggggggctac cagcgccatg gcaaccggct atgttaaagt catcttcttt
      541 tgtcttggat tgtgctatgg cgcgaacaca ttttttcacg ccgccaaagc atatatcgag
      601 ggttatcata ctgtgccaaa gggtcggtgc cgccaggtcg tgaccggcat ggcatggctg
      661 tttttcgtga gctggggtat gttcccaatt ctcttcattt tggggcccga aggttttggc
      721 gtcctgagcg tctatggctc caccgtaggt cacacgatta ttgatctgat gagtaaagag
      781 tgttgggggt tgttgggaca ctacctgcgc gtcctgatcc acgagcacat attgattcac
      841 ggagatatcc gcaaaaccac caaactgaac atcggcggaa cggagatcga ggtcgagact
      901 ctcgtcgaag acgaagccga ggccggagcc gtg
//
