LOCUS       sequence-36486-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Arnold CP, Tan R, Zhou B, Yue SB, Schaffert S, Biggs JR, Doyonnas R,
            Lo MC, Perry JM, Renault VM, Sacco A, Somervaille T, Viatour P, 
            Brunet A, Cleary ML, Li L, Sage J, Zhang DE, Blau HM, Chen C, Chen 
            CZ
  TITLE     MicroRNA programs in normal and aberrant stem and progenitor cells.
  JOURNAL   Genome Res. 2011 May . 21(5):798-810.
  PUBMED    21451113
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2011 May . 21(5):798-810."
COMMENT     Sequence Label: MDH1-miR-31-PGK-GFP-Blast
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    107..568
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(163..180)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     390..409
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ggcnnctggc tggngagctg tacagtaatg aggatcaccg ggcaattgaa gatctagtcg
       61 accaattccg gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
      121 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
      181 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
      241 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
      301 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
      361 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
      421 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
      481 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
      541 ggttttcctt tgaaaaacac gatgataata ccatgaaaac atttaacatt tctcaacagg
      601 atctagaatt agtagaagta gcgacagaga agattacaat gctttatgag gataataaac
      661 atcatgtggg agcggcaatt cgtacgaaaa caggagaaat catttcggca gtacatattg
      721 aagcgtatat aggacgagta actgtttgtg cagaagccat tgcgattggt agtgcagttt
      781 cgaatggaca aaaggatttt gacacgattg tagctgttag acacccttat tctgacgaag
      841 tagatagaag tattcgagtg gtaagtcctt gtggtatgtg tagggagttg atttcagact
      901 atgcaccaga ttgttttgta agangangat ntcaatcnnn antcnatcaa
//
