LOCUS       sequence-364877-        8561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces 
            cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y
  TITLE     Establishment of strigolactone-producing bacterium-yeast consortium.
  JOURNAL   Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. 
            Epub 2021 Sep 17.
  PUBMED    34533983
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-09-17"; volume: "7(38):eabh4048. doi"; pages: " 
            10.1126/sciadv.abh4048. Epub 2021 Sep 17"
COMMENT     Sequence Label: p415PGK1-AtMAX1(pYL759)
FEATURES             Location/Qualifiers
     source          1..8561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    81..584
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        621..725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(726..794,795..1586)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(944..963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1757..2345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2246..2265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2499..2516
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2633..2654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2669..2674,2675..2692,2693..2699)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2707..2723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2712..2734
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2731..2747
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2731..2747
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2766..2786
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2768..2786
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2817..2841
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5405..5429)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5465..5484)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5466..5484)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5494..5511)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5494..5510)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5503..5525)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5651..6106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5738..5757)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5948..5969
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6407..6814
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6815..7909
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(8403..8422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8522..8544
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttaggacg gatcgcttgc ctgtaactta cacgcgcctc gtatctttta
      121 atgatggaat aatttgggaa tttactctgt gtttatttat ttttatgttt tgtatttgga
      181 ttttagaaag taaataaaga aggtagaaga gttacggaat gaagaaaaaa aaataaacaa
      241 aggtttaaaa aatttcaaca aaaagcgtac tttacatata tatttattag acaagaaaag
      301 cagattaaat agatatacat tcgattaacg ataagtaaaa tgtaaaatca caggattttc
      361 gtgtgtggtc ttctacacag acaagatgaa acaattcggc attaatacct gagagcagga
      421 agagcaagat aaaaggtagt atttgttggc gatcccccta gagtctttta catcttcgga
      481 aaacaaaaac tattttttct ttaatttctt tttttacttt ctatttttaa tttatatatt
      541 tatattaaaa aatttaaatt ataattattt ttatagcacg tgatgaaaag gacccaggtg
      601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      901 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      961 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1021 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1081 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1141 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1201 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1261 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1321 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1381 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1441 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1501 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1561 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1621 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1681 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1741 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1801 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1861 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1921 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1981 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2041 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2101 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2161 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2221 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2281 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2341 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2401 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2461 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2521 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2581 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2641 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2701 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2761 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctcccgcgg cccatcacaa
     2821 gtttgtacaa aaaagcaggc taggcatttg caagaattac tcgtgagtaa ggaaagagtg
     2881 aggaactatc gcatacctgc atttaaagat gccgatttgg gcgcgaatcc tttattttgg
     2941 cttcaccctc atactattat cagggccaga aaaaggaagt gtttccctcc ttcttgaatt
     3001 gatgttaccc tcataaagca cgtggcctct tatcgagaaa gaaattaccg tcgctcgtga
     3061 tttgtttgca aaaagaacaa aactgaaaaa acccagacac gctcgacttc ctgtcttcct
     3121 attgattgca gcttccaatt tcgtcacaca acaaggtcct agcgacggct cacaggtttt
     3181 gtaacaagca atcgaaggtt ctggaatggc gggaaagggt ttagtaccac atgctatgat
     3241 gcccactgtg atctccagag caaagttcgt tcgatcgtac tgttactctc tctctttcaa
     3301 acagaattgt ccgaatcgtg tgacaacaac agcctgttct cacacactct tttcttctaa
     3361 ccaagggggt ggtttagttt agtagaacct cgtgaaactt acatttacat atatataaac
     3421 ttgcataaat tggtcaatgc aagaaataca tatttggtct tttctaattc gtagtttttc
     3481 aagttcttag atgctttctt tttctctttt ttacagatca tcaaggaagt aattatctac
     3541 tttttacaac aaatatatac aatgaagacc caacatcaat ggtgggaagt tttggatcca
     3601 ttcttgactc aacatgaagc tttgattgct ttcttgactt tcgctgctgt tgttatcgtt
     3661 atctacttgt atagaccatc ttggtctgtc tgtaatgttc caggtccaac tgctatgcca
     3721 ttggttggtc atttgccatt gatggctaaa tatggtccag atgttttctc cgttttggct
     3781 aaacaatacg gtccaatctt cagattccaa atgggtagac aacccttgat tattatcgct
     3841 gaagctgaat tgtgtagaga ggttggtatt aagaagttca aggacttgcc aaacagatcc
     3901 attccatctc caatttctgc ttctccattg cataagaagg gtttgttttt caccagagac
     3961 aagagatggt caaagatgag aaacaccatc ctgtcattat accagccatc tcatttgacc
     4021 tcattgattc caactatgca ctcctttatt acctctgcta ctcacaactt ggattccaaa
     4081 cctagagata tcgtgttctc caacttgttc ttgaagttga ccaccgatat tattggtcaa
     4141 gctgcttttg gtgttgactt tggtttgtct ggtaaaaagc caatcaagga tgttgaagtt
     4201 accgacttca tcaaccagca tgtttactct actacccaat tgaagatgga cttgtctggt
     4261 tccttgtcca ttattttggg tttgttgatc ccaatcctgc aagaaccttt tagacaagtc
     4321 ttgaagagaa tcccaggtac tatggattgg agagttgaaa agactaacgc tagattgtct
     4381 ggtcagttga acgaaatcgt ttccaagaga gctaaagaag ctgaaaccga ttctaaggac
     4441 ttcctgtctt tgattttgaa ggccagagaa tctgatccat tcgccaagaa tattttcacc
     4501 tccgattaca tttctgccgt tacctatgaa catttgttgg ctggttctgc tactaccgct
     4561 tttactttat cttctgtcct gtacttggtt tccggtcact tagatgtcga aaagagactg
     4621 ttgcaagaaa tcgatggttt cggtaacaga gatttgattc ctactgctca tgacttgcaa
     4681 cataagttcc catacttgga ccaagttatc aaagaagcca tgaggttcta tatggtgtct
     4741 ccattagttg ctagagaaac cgcaaaagaa gttgaaattg gcggttactt gttgccaaaa
     4801 ggtacttggg tttggttggc tttgggtgtt ttggcaaaag atccaaagaa tttcccagaa
     4861 ccagaaaagt tcaagccaga aagatttgat ccaaacggtg aagaagaaaa gcacagacat
     4921 ccatacgctt ttattccatt tggtattggt cctagagctt gtgttggaca aagatttgct
     4981 ctacaagaaa tcaagttgac cctgttgcac ttgtacagaa actacatctt cagacacagc
     5041 ctggaaatgg aaataccatt gcaattggat tacggcatca tcctgagttt taagaacggt
     5101 gttaagttga gaaccatcaa gaggttctga gtgattttgt ataactaaat aatattggaa
     5161 actaaatacg aatacccaaa ttttttatct aaattttgcc gaaagattaa aatctgcaga
     5221 gatatccgaa acaggtaaat ggatgtttca atccctgtag tcagtcagga acccatatta
     5281 tattacagta ttagtcgccg cttaggcacg cctttaatta gcaaaatcaa accttaagtg
     5341 catatgccgt ataagggaaa ctcaaagaac tggcatcgca aaaatgaaaa aaaggaagag
     5401 tgaaacccag ctttcttgta caaagtggtg atggggcggc cgcgtttaaa cggtacccaa
     5461 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     5521 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     5581 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5641 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5701 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5761 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5821 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5881 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5941 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6001 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6061 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     6121 ttctccttac gcatctgtgc ggtatttcac accgcatatc gaccggtcga ggagaacttc
     6181 tagtatatct acatacctaa tattattgcc ttattaaaaa tggaatccca acaattacat
     6241 caaaatccac attctcttca aaatcaattg tcctgtactt ccttgttcat gtgtgttcaa
     6301 aaacgttata tttataggat aattatactc tatttctcaa caagtaattg gttgtttggc
     6361 cgagcggtct aaggcgcctg attcaagaaa tatcttgacc gcagttaact gtgggaatac
     6421 tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa ccattatttt tttcctcaac
     6481 ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact ggaaattttt
     6541 tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat tgggagaaaa
     6601 aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaggcg ttcatgacta
     6661 aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc
     6721 aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat
     6781 atatatatat atatttcaag gatataccat tctaatgtct gcccctaaga agatcgtcgt
     6841 tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg ttcttaaagc
     6901 tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa ttggtggtgc
     6961 tgctatcgat gctacaggtg ttccacttcc agatgaggcg ctggaagcct ccaagaaggc
     7021 tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggtaccggta gtgttagacc
     7081 tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact taagaccatg
     7141 taactttgca tccgactctc ttttagactt atctccaatc aagccacaat ttgctaaagg
     7201 tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta agagaaagga
     7261 agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag aagtgcaaag
     7321 aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc ctatttggtc
     7381 cttggataaa gctaatgttt tggcctcttc aagattatgg agaaaaactg tggaggaaac
     7441 catcaagaac gaattcccta cattgaaggt tcaacatcaa ttgattgatt ctgccgccat
     7501 gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca acatgtttgg
     7561 tgatatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt tgccatctgc
     7621 gtccttggcc tctttgccag acaagaacac cgcgtttggt ttgtacgaac catgccacgg
     7681 ttctgctcca gatttgccaa agaataaggt caaccctatc gccactatct tgtctgctgc
     7741 aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg aagatgcagt
     7801 taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca acagtaccac
     7861 cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa aagattctct
     7921 ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa acgacacgaa
     7981 attacaaaat ggaatatgtt catagggtag acgaaactat atacgcaatc tacatacatt
     8041 tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt gatactaatg
     8101 gctcaacgtg ataaggaaaa agaattgcac tttaacatta atattgacaa ggaggagggc
     8161 accacacaaa aagttaggtg taacagaaaa tcatgaaact atgattccta atttatatat
     8221 tggaggattt tctctaaaaa aaaaaaaata caacaaataa aaaacactca atgacctgac
     8281 catttgatgg agtttaagtc aataccttct tgaaccattt cccataatgg tgaaagttcc
     8341 ctcaagaatt ttactctgtc agaaacggcc ttaacgacgt agtcgatatg gtgcactctc
     8401 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     8461 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     8521 tccgggagct gcatgtgtca gaggttttca ccgtcatcac c
//
