LOCUS       sequence-36490-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Arnold CP, Tan R, Zhou B, Yue SB, Schaffert S, Biggs JR, Doyonnas R,
            Lo MC, Perry JM, Renault VM, Sacco A, Somervaille T, Viatour P, 
            Brunet A, Cleary ML, Li L, Sage J, Zhang DE, Blau HM, Chen C, Chen 
            CZ
  TITLE     MicroRNA programs in normal and aberrant stem and progenitor cells.
  JOURNAL   Genome Res. 2011 May . 21(5):798-810.
  PUBMED    21451113
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2011 May . 21(5):798-810."
COMMENT     Sequence Label: MDH1-miR-221-PGK-GFP-Blast
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    106..567
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(162..179)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     389..408
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gnannctggc tggagagctg tcagtaatga ggatcaccgg gcaattgaag atctagtcga
       61 ccaattccgg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
      121 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
      181 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
      241 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
      301 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
      361 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
      421 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
      481 ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg
      541 gttttccttt gaaaaacacg atgataatac catgaaaaca tttaacattt ctcaacagga
      601 tctagaatta gtagaagtag cgacagagaa gattacaatg ctttatgagg ataataaaca
      661 tcatgtggga gcggcaattc gtacgaaaac aggagaaatc atttcggcag tacatattga
      721 agcgtatata ggacgagtaa ctgtttgtgc agaagccatt gcgattggta gtgcagtttc
      781 gaatggacaa aaggattttg acacgattgt agctgttaga cacccttatt ctgacgaagt
      841 agatagaagt attcgagtgg taagtccttg tggtatgtgt agggagttga tttcagacta
      901 tgcaccagat tgttttgtgt taatagaaat gaatggcaag ttagtcaaaa ctacatgaaa
      961 tctcncaaac
//
