LOCUS       sequence-364916-        6975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. 
            Centromeric URA, PPGK1-OsCYP711A2-Tpho5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y
  TITLE     Establishment of strigolactone-producing bacterium-yeast consortium.
  JOURNAL   Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. 
            Epub 2021 Sep 17.
  PUBMED    34533983
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-09-17"; volume: "7(38):eabh4048. doi"; pages: " 
            10.1126/sciadv.abh4048. Epub 2021 Sep 17"
COMMENT     Sequence Label: p416PGK1-OsCYP711A2(pYL770)
FEATURES             Location/Qualifiers
     source          1..6975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..32)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        100..204
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(205..273,274..1065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(423..442)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1236..1824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1725..1744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1978..1995
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2112..2133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2148..2153,2154..2171,2172..2178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2186..2202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2191..2213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2210..2226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2210..2226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2245..2265
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2247..2265
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2296..2320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4934..4958)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4994..5013)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4995..5013)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5023..5040)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5023..5039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5032..5054)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5180..5635
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5267..5286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5477..5498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5766..6569)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6570..6786)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6810..6829)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6929..6951
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
       61 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      121 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      181 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      241 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      301 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      361 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      421 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      481 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      541 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      601 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      661 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      721 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      781 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      841 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      901 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      961 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1021 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1081 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1141 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1201 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1261 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1321 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1381 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1441 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1501 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1561 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1621 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1681 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1741 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1801 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1861 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1921 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1981 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2041 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2101 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     2161 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     2221 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     2281 ctcccgcggc ccatcacaag tttgtacaaa aaagcaggct aggcatttgc aagaattact
     2341 cgtgagtaag gaaagagtga ggaactatcg catacctgca tttaaagatg ccgatttggg
     2401 cgcgaatcct ttattttggc ttcaccctca tactattatc agggccagaa aaaggaagtg
     2461 tttccctcct tcttgaattg atgttaccct cataaagcac gtggcctctt atcgagaaag
     2521 aaattaccgt cgctcgtgat ttgtttgcaa aaagaacaaa actgaaaaaa cccagacacg
     2581 ctcgacttcc tgtcttccta ttgattgcag cttccaattt cgtcacacaa caaggtccta
     2641 gcgacggctc acaggttttg taacaagcaa tcgaaggttc tggaatggcg ggaaagggtt
     2701 tagtaccaca tgctatgatg cccactgtga tctccagagc aaagttcgtt cgatcgtact
     2761 gttactctct ctctttcaaa cagaattgtc cgaatcgtgt gacaacaaca gcctgttctc
     2821 acacactctt ttcttctaac caagggggtg gtttagttta gtagaacctc gtgaaactta
     2881 catttacata tatataaact tgcataaatt ggtcaatgca agaaatacat atttggtctt
     2941 ttctaattcg tagtttttca agttcttaga tgctttcttt ttctcttttt tacagatcat
     3001 caaggaagta attatctact ttttacaaca aatatataca atggaaatct ctactgtttt
     3061 gggtgctatt ttggctgaat acgctgttac tttggttgct atggctgttg gttttttggt
     3121 tgttggttac ttgtatgagc catactggaa ggttagacat gttccaggtc cagttccatt
     3181 gccattgatt ggtcacttgc atttgttggc tatgcatggt ccagatgttt tctctgtttt
     3241 gactagaaag tacggtccaa tcttcagatt ccatatgggt agacaaccat tggttatggt
     3301 tgctgatgct gaattgtgta aagaagttgg cgttaagaag ttcaagaact tcccaaatag
     3361 atccatgcca tctccaatta ccaattctcc agttcatcaa aagggtctgt ttttcacttc
     3421 tggttctaga tggactacca tgagaaacat gatcttgtct atctaccaac catctcattt
     3481 ggctaccttg attccttcta tggaatcctg tattgaaaga gctgctgaaa acttggaagg
     3541 tcaagaagaa atcaacttct ccaagctgtc tttgtctttc actaccgatg ttttaggtca
     3601 agctgctttt ggtactgatt tcggtttgtc taagaaattg gcctcctctg atgatgatga
     3661 agataccaga aaaattgctg ctgatacttg tgctgaagct aaagcttctt ccgaattcat
     3721 caagatgcat gttcatgcta ccacctcatt gaagatggat atgtctggtt ccttgtccat
     3781 tatcgtcggt caattattgc cattcttgca cgaacctttc agacaagttt tgaagagatt
     3841 gagatggact gccgatcacg aaattgatag agttaatttg actctgggca gacagttgga
     3901 tagaatagtt gctgaaagaa cagctgccat gaagagagat ccagctgctt tacaacaaag
     3961 aaaggatttc ttgtccgtta tgttgaccgc tagggaatct aacaaatctt ctagagaatt
     4021 actgacgccc gattacattt ctgctttgac ttatgaacat ctgttggctg gttctgctac
     4081 tactgctttt actttgacta ctgccttgta cttggttgcc aaacatccag aagttgaaga
     4141 aaagttgttg agggaaattg atggtttcgg tccaagagat agagttccaa ctgctgaaga
     4201 tctgcaaact aagtttccat acttggacca ggttttgaaa gaagctatga ggtattaccc
     4261 atcctcacct ttgattgcca gagaattgaa tcaacagttg gaaatcggtg gttacccatt
     4321 gccaaaaggt acttgggttt ggatggctcc aggtgtattg ggtaaagatc caaagaattt
     4381 tccagagcca gaagtgttta gaccagaaag atttgatcca aacggtgaag aagaaaagcg
     4441 tagacatcca tatgctttgt tcccatttgg tattggtcct agagcttgta ttggtcaaaa
     4501 gttcgctatc caagaaatga agttgtccgc cattcatttc tacagacact acgtttttag
     4561 accctctcca tcaatggaat ctccaccaga atttgtctac tccatcgttt ctaatttcaa
     4621 gaacggtgct aagttgcagg ttatcaagag acatatctaa tgattttgta taactaaata
     4681 atattggaaa ctaaatacga atacccaaat tttttatcta aattttgccg aaagattaaa
     4741 atctgcagag atatccgaaa caggtaaatg gatgtttcaa tccctgtagt cagtcaggaa
     4801 cccatattat attacagtat tagtcgccgc ttaggcacgc ctttaattag caaaatcaaa
     4861 ccttaagtgc atatgccgta taagggaaac tcaaagaact ggcatcgcaa aaatgaaaaa
     4921 aaggaagagt gaaacccagc tttcttgtac aaagtggtga tggggcggcc gcgtttaaac
     4981 ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     5041 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     5101 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     5161 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5221 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5521 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5581 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     5641 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggg taataactga
     5701 tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca
     5761 gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa
     5821 cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata
     5881 atgtcagatc ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct
     5941 cccttgtcat ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt
     6001 ccacccatgt ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg
     6061 tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct
     6121 aacatcaaaa ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta
     6181 acaatacctg ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg
     6241 tatacacccg cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct
     6301 tcgaagagta aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc
     6361 atggaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat
     6421 gcttcaacta actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt
     6481 gtttgctttt cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca
     6541 gcacgttcct tatatgtagc tttcgacatg atttatcttc gtttcctgca ggtttttgtt
     6601 ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt
     6661 atatatacca atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc
     6721 gaatcaaaaa aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaaatgatga
     6781 attgaaaagg tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6841 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     6901 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     6961 tcatcaccga aacgc
//
