LOCUS       sequence-364982-       16984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV 
            site, human TRF1 and TPP1 each with a zz tag and TEV site, and human
            TIN2 with an MBP affinity tag and TEV site in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16984)
  AUTHORS   Jack A, Kim Y, Strom AR, Lee DSW, Williams B, Schaub JM, Kellogg EH,
            Finkelstein IJ, Ferro LS, Yildiz A, Brangwynne CP
  TITLE     Compartmentalization of telomeres through DNA-scaffolded phase 
            separation.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 
            10.1016/j.devcel.2021.12.017.
  PUBMED    35077681
REFERENCE   2  (bases 1 to 16984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "277-290.e9"
COMMENT     Sequence Label: pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV T...
FEATURES             Location/Qualifiers
     source          1..16984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     110..129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     363..380
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  514..738
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(805..1338)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1527..1532,1533..1549,1550..1555))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1951..1984)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2082..2130
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2225..2316
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2396..2569
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2570..2743
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             2801..2821
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2843..2863
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    4313..4447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4806..4854
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4949..5040
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             5120..5293
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             5294..5467
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     TEAEKLNDAQAPK"
     CDS             5525..5545
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5567..5587
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    7658..7792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7948..8039
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             8119..8292
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             8293..8466
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             8524..8544
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             8566..8586
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    10093..10227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        10383..10474
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             10530..10547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             10557..11657
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     11631..11654
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             11712..11732
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    12927..13061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  13377..13542
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        13927..14029
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             14030..14689
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(14096..14115)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      14989..15443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15076..15095)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15285..15306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        15469..15573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15574..15642,15643..16434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15792..15811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(16605..16984,1..209)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
       61 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      121 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      181 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      241 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      301 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      361 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
      421 atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg
      481 ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact
      541 gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac
      601 tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa
      661 actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc
      721 atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
      781 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
      841 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
      901 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
      961 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     1021 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     1081 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     1141 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     1201 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     1261 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     1321 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     1381 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     1441 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     1501 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     1561 tttgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     1621 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     1681 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgcccttgc
     1741 ttcaggagat cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     1801 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     1861 gctatagttc tagtggttgg ctacagcttt gtttgtacta tcaacaggtt gaactgctga
     1921 tcaacagatc ctctacgcgg ccgcggtacc ataacttcgt atagcataca ttatacgaag
     1981 ttatctggcc taggttaatt aaacgctcta tggtctaaag tttttatcga ggctaactga
     2041 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     2101 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     2161 actctgtcaa gtgaacgctc tatggtctaa agatttaaat cgacctactc cggattatta
     2221 atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
     2281 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
     2341 accatcgggc gcggatccaa tgtgcatggc aggccttgcg caacacgatg aagccgtgga
     2401 caacaaattc aacaaagaac aacaaaacgc gtactatgag atcttacatt tacctaactt
     2461 aaacgaagga caacgaaacg ccttcatcca aagtttaaaa gatgacccaa gccaaagcgc
     2521 taacctttta gcagaagcta aaaagctaaa tgatgctcag gcgccgaaag tagacaacaa
     2581 attcaacaaa gaacaacaaa acgcgttcta tgagatctta catttaccta acttaaacga
     2641 agaacaacga aacgccttca tccaaagttt aaaagatgac ccaagccaaa gcgctaacct
     2701 tttagcagaa gctgaaaagc taaatgatgc tcaggcgccg aaagtagacg caaattccgc
     2761 ggggaagtca accgattatg atattccaac tactgctagc gagaacctat atttccaggg
     2821 agattatgat atccctacga ctgagaatct ttattttcag ggcatggcgg aggatgtttc
     2881 ctcagcggcc ccgagcccgc ggggctgtgc ggatggtagg gatgccgacc ctactgagga
     2941 gcagatggca gaaacagaga gaaacgacga ggagcagttc gaatgccagg aactgctcga
     3001 gtgccaggtg caggtggggg cccccgagga ggaggaggag gaggaggagg acgcgggcct
     3061 ggtggccgag gccgaggccg tggctgccgg ctggatgctc gatttcctct gcctctctct
     3121 ttgccgagct ttccgcgacg gccgctccga ggacttccgc aggacccgca acagcgcaga
     3181 ggctattatt catggactat ccagtctaac agcttgccag ttgagaacga tatacatatg
     3241 tcagtttttg acaagaattg cagcaggaaa aacccttgat gcacagtttg aaaatgatga
     3301 acgaattaca cccttggaat cagccctgat gatttggggt tcaattgaaa aggaacatga
     3361 caaacttcat gaagaaatac agaatttaat taaaattcag gctatagctg tttgtatgga
     3421 aaatggcaac tttaaagaag cagaagaagt ctttgaaaga atatttggtg atccaaattc
     3481 tcatatgcct ttcaaaagca aattgcttat gataatctct cagaaagata catttcattc
     3541 cttttttcaa cacttcagct acaaccacat gatggagaaa attaagagtt atgtgaatta
     3601 tgtgctaagt gaaaaatcat caacctttct aatgaaggca gcggcaaaag tagtagaaag
     3661 caaaaggaca agaacaataa cttctcaaga taaacctagt ggtaatgatg ttgaaatgga
     3721 aactgaagct aatttggata caagaaaaag tgttagtgac aaacagtctg cggtaactga
     3781 atcctcagag ggtacagtat ccttattgag gtctcacaag aatcttttct tatctaagtt
     3841 gcaacatgga acccagcaac aagaccttaa taagaaagaa agaagagtag gaactcctca
     3901 aagtacaaaa aagaaaaaag aaagcagaag agccactgaa agcagaatac ctgtttcaaa
     3961 gagtcagccg gtaactcctg aaaaacatcg agctagaaaa agacaggcat ggctttggga
     4021 agaagacaag aatttgagat ctggcgtgag gaaatatgga gagggaaact ggtctaaaat
     4081 actgttgcat tataaattca acaaccggac aagtgtcatg ttaaaagaca gatggaggac
     4141 catgaagaaa ctaaaactga tttcctcaga cagcgaagac tgaaagcttg tcgagaagta
     4201 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     4261 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     4321 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4381 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4441 ctggatctga tcactgcttg agcctagaag atccggctgc taacaaagcc cgaaaggaag
     4501 ctgagttggc tgctgccacc gctgagcaat aactatcata accatttaaa taaatctata
     4561 tctcaatcgg ggttacgtcg ataccccacc gagaccccat tgggaccaat acgcccgcgt
     4621 ttcttccttt tccccacccc aacccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     4681 cgtcggggcg gcaagccctg ccatagccac tacgggttaa ttaaaaacta tatctcaatc
     4741 ggggttttta tcgaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     4801 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
     4861 gcggggttcg gtcccagggc tggcactctg tcaagtgaac gctctatggt ctaaagattt
     4921 aaatcgacct actccggaat attaatagat catggagata attaaaatga taaccatctc
     4981 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
     5041 attccggatt attcataccg tcccaccatc gggcgcggat ccaatgtgca tggcaggcct
     5101 tgcgcaacac gatgaagccg tggacaacaa attcaacaaa gaacaacaaa acgcgtacta
     5161 tgagatctta catttaccta acttaaacga aggacaacga aacgccttca tccaaagttt
     5221 aaaagatgac ccaagccaaa gcgctaacct tttagcagaa gctaaaaagc taaatgatgc
     5281 tcaggcgccg aaagtagaca acaaattcaa caaagaacaa caaaacgcgt tctatgagat
     5341 cttacattta cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa gtttaaaaga
     5401 tgacccaagc caaagcgcta accttttaac agaagctgaa aagctaaatg atgctcaggc
     5461 gccgaaagta gacgcaaatt ccgcggggaa gtcaaccgat tatgatattc caactactgc
     5521 tagcgagaac ctatatttcc agggagatta tgatatccct acgactgaga atctttattt
     5581 tcagggcgac tcattggagt ttatcgcatc aaagctcgca atgtctttgg ttccagcaac
     5641 aaattatata tatacacccc tgaatcaact taagggtggt acaattgtca atgtctatgg
     5701 tgttgtgaag ttctttaagc ccccatatct aagcaaagga actgattatt gctcagttgt
     5761 aactattgtg gaccagacaa atgtaaaact aacttgcctg ctctttagtg gaaactatga
     5821 agcccttcca ataatttata aaaatggaga tattgttcgc tttcacaggc tgaagattca
     5881 agtatataaa aaggagactc agggtatcac cagctctggc tttgcatctt tgacgtttga
     5941 gggaactttg ggagccccta tcatacctcg cacttcaagc aagtatttta acttcactac
     6001 tgaggaccac aaaatggtag aagccttacg tgtttgggca tctactcata tgtcaccgtc
     6061 ttggacatta ctaaaattgt gtgatgttca gccaatgcag tattttgacc tgacttgtca
     6121 gctcttgggc aaagcagaag tggacggagc atcatttctt ctaaaggtat gggatggcac
     6181 caggacacca tttccatctt ggagagtctt aatacaagac cttgttcttg aaggtgattt
     6241 aagtcacatc catcggctac aaaatctgac aatagacatt ttagtctacg ataaccatgt
     6301 tcatgtggca agatctctga aggttggaag ctttcttaga atctatagcc ttcataccaa
     6361 acttcaatca atgaattcag agaatcagac aatgttaagt ttagagtttc atcttcatgg
     6421 aggtaccagt tacggtcggg gaatcagggt cttgccagaa agtaactctg atgtggatca
     6481 actgaaaaag gatttagaat ctgcaaattt gacagccaat cagcattcag atgttatctg
     6541 tcaatcagaa cctgacgaca gctttccaag ctctggatca gtatcattat acgaggtaga
     6601 aagatgtcaa cagctatctg ctacaatact tacagatcat cagtatttgg agaggacacc
     6661 actatgtgcc attttgaaac aaaaagctcc tcaacaatac cgcatccgag caaaattgag
     6721 gtcatataag cccagaagac tatttcagtc tgttaaactt cattgcccta aatgtcattt
     6781 gctgcaagaa gttccacatg agggcgattt ggatataatt tttcaggatg gtgcaactaa
     6841 aaccccagat gtcaagctac aaaatacatc attatatgat tcaaaaatct ggaccactaa
     6901 aaatcaaaaa ggacgaaaag tagcagttca ttttgtgaaa aataatggta ttctcccgct
     6961 ttcaaatgaa tgtctacttt tgatagaagg aggtacactc agtgaaattt gcaaactctc
     7021 gaacaagttt aatagtgtaa ttcctgtgag atctggccac gaagacctgg aacttttgga
     7081 cctttcagca ccatttctta tacaaggaac aatacatcac tatggatgta aacagtgttc
     7141 tagtttgaga tccatacaaa atctaaattc cctggttgat aaaacatcgt ggattccttc
     7201 ttctgtggca gaagcactgg gtattgtacc cctccaatat gtgtttgtta tgacctttac
     7261 acttgatgat ggaacaggag tactagaagc ctatctcatg gattctgaca aattcttcca
     7321 gattccagca tcagaagttc tgatggatga tgaccttcag aaaagtgtgg atatgatcat
     7381 ggatatgttt tgtcctccag gaataaaaat tgatgcatat ccgtggttgg aatgcttcat
     7441 caagtcatac aatgtcacaa atggaacaga taatcaaatt tgctatcaga tttttgacac
     7501 cacagttgca gaagatgtaa tctaatgaaa gcttgtcgag aagtactaga ggatcataat
     7561 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7621 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     7681 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7741 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     7801 gcttgagcct agaagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     7861 ccaccgctga gcaataacta tcataaccat ttaaataaac tggatactat tgcacgttta
     7921 aatcgaccta ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg
     7981 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
     8041 ttccggatta ttcataccgt cccaccatcg ggcgcggatc caatgtgcat ggcaggcctt
     8101 gcgcaacacg atgaagccgt ggacaacaaa ttcaacaaag aacaacaaaa cgcgtactat
     8161 gagatcttac atttacctaa cttaaacgaa ggacaacgaa acgccttcat ccaaagttta
     8221 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct
     8281 caggcgccga aagtagacaa caaattcaac aaagaacaac aaaacgcgtt ctatgagatc
     8341 ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat
     8401 gacccaagcc aaagcgctaa ccttttagca gaagctgaaa agctaaatga tgctcaggcg
     8461 ccgaaagtag acgcaaattc cgcggggaag tcaaccgatt atgatattcc aactactgct
     8521 agcgagaacc tatatttcca gggagattat gatatcccta cgactgagaa tctttatttt
     8581 cagggcatgg caggttcggg gaggctggtc ctacggccct ggattcggga gctgattctg
     8641 gggtcagaga caccctccag tccacgagcc gggcagctgc ttgaggtact acaggacgcc
     8701 gaggccgcgg tcgcgggccc atcccacgcc cctgatacgt ccgacgtcgg ggccacgctg
     8761 cttgtgtctg acgggaccca cagtgtccga tgcctggtga cgcgggaggc cctggacacc
     8821 tcggactggg aggagaagga gttcggcttc cgcgggacag agggccggct gctgctgctg
     8881 caggactgcg gggttcatgt ccaggtcgct gagggcggcg cgcccgcaga gttctatctc
     8941 caggtggacc gcttcagcct gctgcccacg gagcagcccc ggctacgggt gcctggttgc
     9001 aaccaagact tagatgttca gaaaaagctc tatgactgcc ttgaggagca cctttcagag
     9061 tccacctcgt ccaatgcagg cctatcactg tcccagcttc tggatgaaat gcgggaggac
     9121 caggagcatc agggggcact cgtgtgcctg gctgaaagct gcctgacact ggagggccct
     9181 tgcacagcac cccctgtcac ccactgggct gcctcacgat gcaaggccac gggagaagct
     9241 gtgtacactg tccccagctc aatgctgtgc atctctgaga atgaccagct aattctgagc
     9301 tctctaggcc cctgtcagag gacacagggc cctgagctgc ccccaccaga cccggctctg
     9361 caggacctat ctctgaccct catagcctct cctccttcct cacccagttc ctcaggaacc
     9421 ccggccttac ccggccacat gtcatccgag gaaagtggta ccagcatcag ccttctgcct
     9481 gccctgtcct tggctgctcc agacccaggg cagagaagca gctcccagcc ctcaccagcc
     9541 atctgctcag cccctgccac cctgaccccc aggtccccac acgccagccg tacccccagc
     9601 tccccactcc agagctgcac tcccagtctc tcaccccgta gccatgtccc cagtccacac
     9661 caggctcttg tgaccaggcc ccagaaacct agcctggagt tcaaggagtt tgtagggttg
     9721 ccctgcaaga atcggccgcc ttttcccagg accggagcta ccaggggagc ccaggagccc
     9781 tgctctgtct gggaaccccc aaagaggcat cgtgatggtt ctgccttcca gtatgagtat
     9841 gagccaccct gcacgtccct ctgtgctcgg gtccaagctg tcaggcttcc tccccagctc
     9901 atggcctggg ccttgcactt tctgatggat gcacagccag ggtctgagcc aactccgatg
     9961 tgaaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt
    10021 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    10081 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10141 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10201 atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctagaag atccggctgc
    10261 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactatcata
    10321 accatttaaa taaacctaat gatgcctgat gtttaaatcg acctactccg gaatattaat
    10381 agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt
    10441 tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac
    10501 catcgggcgc ggatccaatg ggttcttctc accatcacca tcaccatggt tcttctatga
    10561 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    10621 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    10681 ataaactgga agagaaattc ccacaggttg cggcaactgg cgatggccct gacattatct
    10741 tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    10801 cggacaaagc gttccaggac aagctgtatc cgtttacctg ggatgccgta cgttacaacg
    10861 gcaagctgat tgcttacccg atcgctgttg aagcgttatc gctgatttat aacaaagatc
    10921 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    10981 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    11041 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    11101 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    11161 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    11221 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    11281 attatggtgt aacggtactg ccgaccttca agggtcaacc atccaaaccg ttcgttggcg
    11341 tgctgagcgc aggtattaac gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg
    11401 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    11461 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacta
    11521 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    11581 atgccgtgcg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    11641 tgaaagacgc gcagactaat tcgagctcga acaacaacaa caataacaat aacaacaacc
    11701 tcgggatcga ggaaaacctg tacttccaat ccgctacgcc cctggtggcg ggtcccgcag
    11761 ctctacgctt cgccgccgcg gctagctggc aggttgtgcg cggacgctgc gtggaacatt
    11821 ttccgcgagt actggagttt ctgcgatctc tgcgcgctgt tgcccctggc ttggttcgct
    11881 accggcacca cgaacgcctt tgtatgggcc taaaggccaa ggtggtggtg gagctgatcc
    11941 tgcagggccg gccttgggcc caagtcctga aagccctgaa tcaccacttt ccagaatctg
    12001 gacctatagt gcgggatccc aaggctacaa agcaggatct gaggaagatt ttggaggcac
    12061 aggaaacttt ttaccagcag gtgaagcagc tgtcagaggc tcctgtggat ttggcctcga
    12121 agctgcagga acttgaacaa gagtatgggg aaccctttct ggctgccatg gaaaagctgc
    12181 tttttgagta cttgtgtcag ctggagaaag cactgcctac accgcaggca cagcagcttc
    12241 aggatgtgct gagttggatg cagcctggag tctctatcac ctcttctctt gcctggagac
    12301 aatatggtgt ggacatgggg tggctgcttc cagagtgctc tgttactgac tcagtgaacc
    12361 tggctgagcc catggaacag aatcctcctc agcaacaaag actagcactc cacaatcccc
    12421 tgccaaaagc caagcctggc acacatcttc ctcagggacc atcttcaagg acgcacccag
    12481 aacctctagc tggccgacac ttcaatctgg cccctctagg ccgacgaaga gttcagtccc
    12541 aatgggcctc cactagggga ggccataagg agcgccccac agtcatgctg tttcccttta
    12601 ggaatctcgg ctcaccaacc caggtcatat ctaagcctga gagcaaggaa gaacatgcga
    12661 tatacacagc agacctagcc atgggcacaa gagcagcctc cactgggaag tctaagagtc
    12721 catgccagac cctgggggga agggctctga aggagaaccc agttgacttg cctgccacag
    12781 agcaaaagga gtaatgaaag cttgtcgaga agtactagag gatcataatc agccatacca
    12841 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    12901 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    12961 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    13021 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta
    13081 gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
    13141 caataactat cataaccatt taaataaatc tatatctcaa tcggggttac gtcgataccc
    13201 caccgagacc ccattgggac caatacgccc gcgtttcttc cttttcccca ccccaacccc
    13261 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaagc cctgccatag
    13321 ccactacggg ttaattaaaa actggatact attgcacgtt tcaatcagga gatccgaacc
    13381 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg
    13441 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc
    13501 atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc
    13561 tgttatgttt ttaatcaaac atcctgccaa ctccatgtga caaaccgtca tcttcggcta
    13621 ctttttctct gtcacagaat gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa
    13681 ttgactgaat atcaacgctt atttgcagcc tgaactgaac aggagggaca gctgatagaa
    13741 acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
    13801 atcacccgac gcactttgcg ccgaataaat acctgtgacg gaagatcact tcgcagaata
    13861 aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg
    13921 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
    13981 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
    14041 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
    14101 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
    14161 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
    14221 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
    14281 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
    14341 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
    14401 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
    14461 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
    14521 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
    14581 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
    14641 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
    14701 cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat
    14761 gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa
    14821 atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc
    14881 gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt
    14941 gacgatatga tcatttattc tgcctcccag agcctgatgg cgaatgggac gcgccctgta
    15001 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
    15061 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
    15121 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
    15181 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
    15241 agacggtttt tcgccctttg acgttggagt ccacgttctt aatagtggac tcttgttcca
    15301 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    15361 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
    15421 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
    15481 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    15541 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    15601 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    15661 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    15721 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    15781 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
    15841 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    15901 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    15961 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    16021 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    16081 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    16141 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    16201 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    16261 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    16321 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    16381 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    16441 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    16501 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    16561 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    16621 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    16681 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    16741 ccaaatacta ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    16801 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    16861 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    16921 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    16981 tacc
//
