LOCUS       sequence-36499-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6347 MSCV-CMV-Flag-HA-Brd4-444-722
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..48
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     58..82
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             164..187
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             200..226
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    242..266
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             290..310
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttagcgactc tcctatagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
       61 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      121 aagacaccgg gaccgatcca gcctccgggg atctgccacc atggactaca aggatgacga
      181 tgacaagctc gatggaggat acccatacga tgttccagat tacgctgctc gaggttatca
      241 aacaagtttg tacaaaaaag caggctccgc ggccgccccc ttcacccctc ctaagaagaa
      301 gaggaaggtg cgcaagctcc aggatgtgtt cgaaatgcgc tttgccaaga tgccggacga
      361 gcctgaggag ccagtggtgg ccgtgtcctc cccggcagtg ccccctccca ccaaggttgt
      421 ggccccgccc tcatccagcg acagcagcag cgatagctcc tcggacagtg acagttcgac
      481 tgatgactct gaggaggagc gagcccagcg gctggctgag ctccaggagc agctcaaagc
      541 cgtgcacgag cagcttgcag ccctctctca gccccagcag aacaaaccaa agaaaaagga
      601 gaaagacaag aaggaaaaga aaaaagaaaa gcacaaaagg aaagaggaag tggaagagaa
      661 taaaaaaagc aaagccaagg aacctcctcc taaaaagacg aagaaaaata atagcagcaa
      721 cagcaatgtg agcaagaagg agccagcgcc catgaagagc aagccccctc ccacgtatga
      781 gtcggaggaa gaggacaagt gcaagcctat gtcctatgag gagaagcggc agctcagctt
      841 ggacatcaac aagctccccg gcgagaagct gggccgcgtg gtgcacatca tccagtcacg
      901 ggagcccttc cctga
//
