LOCUS       sequence-36505-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6352 MSCV-CMV-CMV-Flag-HA-JMJD6
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     65..89
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             171..194
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             207..233
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    249..273
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggctttagca gagctctcct atcagtgata gagatctccc tatcagtgat agagatcgtc
       61 gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      121 tccatagaag acaccgggac cgatccagcc tccggggatc tgccaccatg gactacaagg
      181 atgacgatga caagctcgat ggaggatacc catacgatgt tccagattac gctgctcgag
      241 gttatcaaac aagtttgtac aaaaaagttg gcatgaacca caagagcaag aagcgcatcc
      301 gcgaggccaa gcggagtgcg cggccggagc tcaaggactc gctggattgg acccggcaca
      361 actactacga gagcttctcg ctgagcccgg cggccgtggc ggataacgtg gaaagggcag
      421 atgctttaca gctgtctgtg gaagaatttg tggagcggta tgaaagacct tacaagcccg
      481 tggttttgtt gaatgcgcaa gagggctggt ctgcgcagga gaaatggact ctggagcgcc
      541 taaaaaggaa atatcggaac cagaagttca agtgtggtga ggataacgat ggctactcag
      601 tgaagatgaa gatgaaatac tacatcgagt acatggagag cactcgagat gatagtcccc
      661 tttacatctt tgacagcagc tatggtgaac accctaaaag aaggaaactt ttggaagact
      721 acaaggtgcc aaagtttttc actgatgacc tttttccagt atgct
//
