LOCUS       sequence-365529-        1440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of LuxC via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxC
FEATURES             Location/Qualifiers
     source          1..1440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1440
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 atgactaaaa aaatttcatt cattattaac ggccaggttg aaatctttcc cgaaggtgat
       61 gatttagtgc aatccattaa ttttggtgat aatagtgttt acctgccaat attgaatgac
      121 tctcatgtaa aaaacattat tgattgtaat ggaaataacg aattacggtt gcataacatt
      181 gtcaattttc tctatacggt agggcaaaga tggaaaaatg aagaatactc aagacgcagg
      241 acatacattc gtgacttaaa aaaatatatg ggatattcag aagaaatggc taagctagag
      301 gccaattgga tatctatgat tttatgttct aaaggcggcc tttatgatgt tgtagaaaat
      361 gaacttggtt ctcgccatat catggatgaa tggctacctc aggatgaaag ttatgttcgg
      421 gcttttccga aaggtaaatc tgtacatctg ttggcaggta atgttccatt atctgggatc
      481 atgtctatat tacgcgcaat tttaactaag aatcagtgta ttataaaaac atcgtcaacc
      541 gatcctttta ccgctaatgc attagcgtta agttttattg atgtagaccc taatcatccg
      601 ataacgcgct ctttatctgt tatatattgg ccccaccaag gtgatacatc actcgcaaaa
      661 gaaattatgc aacatgcgga tgttattgtc gcttggggag ggccagatgc gattaattgg
      721 gcggtagagc atgcgccatc ttatgctgat gtgattaaat ttggttctaa aaagagtctt
      781 tgcattatcg ataatcctgt tgatttgacg tccgcagcga caggtgcggc tcatgatgtt
      841 tgtttttacg atcagcgagc ttgtttttct gcccaaaaca tatattacat gggaaatcat
      901 tatgaggaat ttaagttagc gttgatagaa aaacttaatc tatatgcgca tatattaccg
      961 aatgccaaaa aagattttga tgaaaaggcg gcctattctt tagttcaaaa agaaagcttg
     1021 tttgctggat taaaagtaga ggtggatatt catcaacgtt ggatgattat tgagtcaaat
     1081 gcaggtgtgg aatttaatca accacttggc agatgtgtgt accttcatca cgtcgataat
     1141 attgagcaaa tattgcctta tgttcaaaaa aataagacgc aaaccatatc tatttttcct
     1201 tgggagtcat catttaaata tcgagatgcg ttagcattaa aaggtgcgga aaggattgta
     1261 gaagcaggaa tgaataacat atttcgagtt ggtggatctc atgacggaat gagaccgttg
     1321 caacgattag tgacatatat ttctcatgaa aggccatcta actatacggc taaggatgtt
     1381 gcggttgaaa tagaacagac tcgattcctg gaagaagata agttccttgt atttgtccca
//
