LOCUS       sequence-365586-        1150 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GFP-HiBiT via T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-HisGFPHiBiT
FEATURES             Location/Qualifiers
     source          1..1150
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..43
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     rep_origin      357..812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(444..463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     654..675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(879..898)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 anncnnacgn nancgcganc acatggtcct gctggagttc gtgaccgccg ccgggatcgg
       61 aggtggtgga tcgggcggag ggggatcaat ggtgagcggc tggcggctgt tcaagaagat
      121 tagctaactc gagcaaagcc cgtcgagcaa agcccgccga aaggcgggct tttctgtaca
      181 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
      241 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgggctgg cgtaatagcg
      301 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc
      361 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      421 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      481 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      541 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      601 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      661 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      721 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
      781 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
      841 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
      901 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      961 ctcccggcat ccgnntacag acaanctgtg accgnnctcc gggnnctgca tgnntcagnn
     1021 nnttttcacc gnncatcanc ngaaaacgcn nnnanacnna aannnnnncn nngnnnacnn
     1081 nnnnttttnn nnnntnanng tcatngannn nantnnnnnn nnannnngtn nnnnnnnncn
     1141 nnncggggaa
//
