LOCUS       sequence-365662-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for repair templates to tag a gene with a mNeonGreen 
            and mIAA7 AID degron using homology-directed repair for C. elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, 
            Boxem M
  TITLE     The mIAA7 degron improves auxin-mediated degradation in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222.
  PUBMED    36029236
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Aug 27. pii: 6677660. doi: 
            10.1093/g3journal/jkac222."
COMMENT     Sequence Label: pJJS005
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      307..316
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtctcca agggagagga ggacaacatg gcctccctcc cagccaccca cgagctccac
       61 atcttcggat ccatcaacgg agtcgacttc gacatggtcg gacaaggaac cggaaaccca
      121 aacgacggat acgaggagct caacctcaag tccaccaagg taagtttaaa catatatata
      181 ctaactaacc ctgattattt aaattttcag ggagacctcc aattctcccc atggatcctc
      241 gtcccacaca tcggatacgg attccaccaa tacctcccat acccagacgg aatgtcccca
      301 ttccaagccg ccatggtcga cggatccgga taccaagtcc accgtaccat gcaattcgag
      361 gacggagcct ccctcaccgt caactaccgt tacacctacg agggatccca catcaaggta
      421 agtttaaaca gttcggtact aactaaccat acatatttaa attttcaggg agaggcccaa
      481 gtcaagggaa ccggattccc agccgacgga ccagtcatga ccaactccct caccgccgcc
      541 gactggtgcc gttccaagaa gacctaccca aacgacaagg taagtttaaa catgatttta
      601 ctaactaact aatctgattt aaattttcag accatcatct ccaccttcaa gtggtcctac
      661 accaccggaa acggaaagcg ttaccgttcc accgcccgta ccacctacac cttcgccaag
      721 ccaatggccg ccaactacct caagaaccaa ccaatgtacg tcttccgtaa gaccgagctc
      781 aagcactcca agaccgagct caacttcaag gagtggcaaa aggccttcac cgacgtcatg
      841 ggaatggacg agctctacaa g
//
