LOCUS       sequence-365705-        6782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector packaging small FRET-based near-infrared 
            genetically-encoded calcium indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Matlashov ME, Vera J, Kasatkina LA, Khodakhah K, Verkhusha VV
  TITLE     Design and Initial Characterization of a Small Near-Infrared 
            Fluorescent Calcium Indicator.
  JOURNAL   Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 
            10.3389/fcell.2022.880107. eCollection 2022.
  PUBMED    35846350
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2022-06-29"; volume: "10:880107. doi"; pages: " 
            10.3389/fcell.2022.880107. eCollection 2022"
COMMENT     Sequence Label: pAAV-CAG-iGECInano
FEATURES             Location/Qualifiers
     source          1..6782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   105..234
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        314..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1211..1233
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1295..1314
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1357..1366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3013..3601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3066..3086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3484..3495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3633..4109
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3832..3851)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4149..4289
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4149..4278
                     /label=AAV2 ITR
     rep_origin      4364..4819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4451..4470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4661..4682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4836..4855)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4955..4977
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5015..5033)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5101..5205
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5206..5274,5275..6066)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5424..5443)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6237..6782,1..43)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6726..6745
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
       61 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcctgca ggcagctgcg
      121 cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag
      181 tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt tcctgcggcc
      241 gcacgcgtaa cactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
      661 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
      721 gggcgggggg gggggggggg gcggggcggg gcgaggggyr krrsrgrgcg aggcggagag
      781 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      841 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
      901 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
      961 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     1021 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     1081 agcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     1141 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     1201 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     1261 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     1321 catcattttg gcaaagaatt gcggcccaac ggtaccgcca ccatgggcat taacgagctt
     1381 gctcttaagt tggctggact tgatattaac aagactggag gaggttctaa tctcgataaa
     1441 atgctcaata caacagtgac cgaggtgcgc aagttcctgc aagcggacag agtgtgcgtg
     1501 ttcaagttcg aggaagatta ctccggcacc gtctcgcacg aagccgtgga cgacagatgg
     1561 attagcatcc tgaagaccca ggtgcaggac agatacttca tggaaaccag aggcgaggaa
     1621 tacgtccacg gcagatacca ggccatcgcc gacatctaca cagccaatct ggtcgagtgc
     1681 tacagagacc tgctgatcga gtttcaggtg cgggccattc tggctgtccc catcctgcaa
     1741 ggcaagaagc tgtggggcct gctggtggcc caccaactgg ccggccctcg ggagtggcag
     1801 acctgggaaa tcgacttcct gaaacagcaa gctgtcgtca tgggaattgc tattcaacag
     1861 ccgccaagcg aagaggaact gagcgagtgc ttcagaatct tcgacttcga cggcaacggc
     1921 ttcatcgaca gagaggagtt tggcgacatc atcagactga ccggcgagca gctgaccgac
     1981 gaggacgtgg acgagatctt cggcgactcc gacaccgaca agaacggcag aatcgatttc
     2041 gacgagttcc tgaagatggt ggaaaacgtg cagccgacct gcgacgatga gccgatccat
     2101 atccccggtg ccatccaacc gcatggactg ctgctcgccc tcgccgccga catgacgatc
     2161 gttgccggca gcgacaacct tcccgaactc accggactgg cgatcggcgc cctgatcggc
     2221 cgctctgcgg ccgatgtctt cgactcggag acgcacaacc gtctgacgat cgccttggcc
     2281 gagcccgggg cggccgtcgg agcaccgatc actgtcggct tcacgatgcg aaaggacgca
     2341 ggcttcatcg gctcctggca tcgccatgat cagctcatct tcctggagct ggagcctccc
     2401 cagcgggacg tcgccgagcc gcaggcgttc ttccgccgca ccaacagcgc catccgccgc
     2461 ctgcaggccg ccgaaacctt ggaaagcgcc tgcgccgccg cggcgcaaga ggtgcggaag
     2521 attaccggct tcgatcgggt gatgatctat cgcttcgcct ccgacttcag cgggtccgtg
     2581 atcgcagagg atcggtgcgc cgaggtcgag tcaaaactag gcctgcacta tcctgcctca
     2641 ttcatcccgg cgcaggcccg tcggctctat accatcaacc cggtacggat cattcccgac
     2701 atcaattatc ggccggtgcc ggtcacccca gacctcaatc cggtcaccgg gcggccgatt
     2761 gatcttagct tcgccatcct gcgcagcgtc tcgcccaacc atctggagtt catgcgcaac
     2821 ataggcatgc acggcacgat gtcgatctcg attttgcgcg gcgagcgact gtggggattg
     2881 atcgtttgcc atcaccgaac gccgtactac gtcgatctcg atggccgcca agcctgcaag
     2941 agggtcgccg agaggctggc cactcagatc ggcgtgatgg aagagtgaga attcgatatc
     3001 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3061 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3121 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3181 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3241 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3301 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3361 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3421 ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     3481 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3541 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3601 catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag
     3661 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     3721 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     3781 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     3841 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     3901 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     3961 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     4021 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     4081 cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga
     4141 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4201 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4261 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     4321 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     4381 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4441 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4501 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4561 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4621 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4681 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4741 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4801 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4861 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4921 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4981 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5041 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5101 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5161 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5221 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5281 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5341 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5401 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5461 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5521 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5581 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5641 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5701 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5761 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5821 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5881 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5941 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6001 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6061 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6121 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6181 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6241 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6301 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6361 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6421 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6481 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6541 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6601 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6661 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6721 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6781 cg
//
