LOCUS       sequence-365763-        4479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A14_STOP
FEATURES             Location/Qualifiers
     source          1..4479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2036..2135)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2152..2171)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2153..2171)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2176..2192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2176..2192)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2294..2313)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2305..3114
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2372..2391)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3261..3849
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3750..3769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4003..4020
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4179..4206)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4298..4384)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4433..4455
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4447..4464
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4448..4464
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga caagctgaag
      121 tgcccttctt tctttaagtg tagggagaag gagaaggtga gcgccagctc cgagaacttc
      181 cacgtgggcg agaacgacga gaatcaggat cgcggcaatt ggtccaagaa gtctgattac
      241 ctgctgtcta tgatcggcta tgccgtgggc ctgggcaacg tgtggaggtt cccatacctg
      301 acatatagca atggcggcgg cgcctttctg atcccatacg caatcatgct ggccctggca
      361 ggactgcccc tgttctttct ggagtgctcc ctgggacagt ttgcaagcct gggaccagtg
      421 tccgtgtgga ggatcctgcc actgttccag ggagtgggca tcacaatggt gctgatctcc
      481 atctttgtga ccatctacta taacgtgatc atcgcctatt ctctgtacta tatgtttgcc
      541 agcttccagt ccgagctgcc ttggaagaac tgctctagct ggagcgacaa gaattgttcc
      601 cggtctccaa tcgtgacaca ctgcaacgtg agcaccgtga ataagggcat ccaggagatc
      661 atccagatga acaagtcctg ggtggatatc aacaatttca catgtatcaa tggcagcgag
      721 atctaccagc ctggccagct gccatccgag cagtattgga acaaggtggc cctgcagcgg
      781 tcctctggca tgaatgagac cggcgtgatc gtgtggtacc tggccctgtg cctgctgctg
      841 gcatggctga tcgtgggagc cgccctgttt aagggcatca agagctccgg caaggtggtg
      901 tacttcacag ccctgtttcc ctatgtggtg ctgctgatcc tgctggtgag aggagccacc
      961 ctggagggag catctaaggg aatcagctac tatatcggcg cccagtccaa cttcacaaag
     1021 ctgaaggagg cagaagtgtg gaaggacgca gcaacccaga tcttttacag cctgtccgtg
     1081 gcatggggag gactggtggc cctgtctagc tacaacaagt tcaagaacaa ctgttttagc
     1141 gatgccatcg tggtgtgcct gacaaattgt ctgacctccg tgtttgccgg cttcgccatc
     1201 ttttctatcc tgggccacat ggcccacatc tctggcaagg aggtgagcca ggtggtgaag
     1261 tccggcttcg acctggcctt tatcgcctac cctgaggccc tggcacagct gccaggagga
     1321 ccattctggt ccatcctgtt ctttttcatg ctgctgacac tgggcctgga ctcccagttc
     1381 gcctctatcg agaccatcac cacaaccatc caggatctgt ttcccaaagt gatgaagaag
     1441 atgagggtgc ctatcacact gggctgctgt ctggtgctgt tcctgctggg actggtgtgc
     1501 gtgacccagg caggcatcta ctgggtgcac ctgatcgacc acttttgtgc aggatgggga
     1561 atcctgatcg cagcaatcct ggagctggtg ggcatcatct ggatctatgg cggcaaccgg
     1621 ttcatcgagg atacagagat gatgatcggc gccaagcggt ggatcttctg gctgtggtgg
     1681 agagcctgct ggtttgtgat cacccccatc ctgctgatcg ccatcttcat ctggagcctg
     1741 gtgcagtttc acagacctaa ttacggcgcc atcccatatc ctgactgggg agtggccctg
     1801 ggatggtgca tgatcgtgtt ctgtatcatc tggatcccaa tcatggccat catcaagatc
     1861 atccaggcca agggcaacat ctttcagagg ctgatctctt gctgtcgccc cgccagcaat
     1921 tggggacctt acctggagca gcacagggga gagagatata aggacatggt ggatcccaag
     1981 aaggaggccg atcacgagat ccctaccgtg tctggcagcc gcaagccaga gtaagaccca
     2041 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2101 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2161 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2221 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2281 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2341 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2401 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2461 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2521 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2581 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2641 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2701 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2761 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2821 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2881 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2941 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3001 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3061 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3121 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3181 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3241 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3301 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3361 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3421 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3481 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3541 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3601 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3661 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3721 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3781 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3841 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3901 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3961 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4021 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4081 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4141 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4201 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4261 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4321 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4381 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4441 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
