LOCUS       sequence-365769-        3864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC10A4_STOP
FEATURES             Location/Qualifiers
     source          1..3864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1421..1520)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1537..1556)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1538..1556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1561..1577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1561..1577)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1679..1698)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1690..2499
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1757..1776)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2646..3234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3135..3154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3388..3405
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3564..3591)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3683..3769)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3818..3840
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3832..3849
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3833..3849
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga cggcaacgat
      121 aatgtgacac tgctgttcgc ccccctgctg agggacaact acaccctggc accaaatgca
      181 agctccctgg gacctggaac agatctggcc ctggcacctg catctagcgc cggacctggc
      241 ccaggactgt ccctgggacc aggaccttct ttcggcttta gcccaggacc aacccctaca
      301 ccagagccaa ccacatctgg actggcagga ggagcagcat cccacggacc ttctccattc
      361 ccaagacctt gggcaccaca cgcactgccc ttttgggaca cccccctgaa ccacggcctg
      421 aacgtgttcg tgggagccgc cctgtgcatc acaatgctgg gcctgggctg taccgtggat
      481 gtgaaccact tcggagcaca cgtgcggaga cctgtgggcg ccctgctggc cgccctgtgc
      541 cagtttggac tgctgccact gctggccttc ctgctggccc tggcctttaa gctggacgag
      601 gtggcagcag tggccgtgct gctgtgcgga tgctgtcctg gcggcaacct gagcaatctg
      661 atgtccctgc tggtggacgg cgatatgaac ctgagcatca tcatgacaat ctcctctacc
      721 ctgctggccc tggtgctgat gcctctgtgc ctgtggatct attcctgggc ctggatcaat
      781 acccctatcg tgcagctgct gccactgggc accgtgaccc tgaccctgtg cagcacactg
      841 atcccaatcg gcctgggcgt gttcatcagg tacaagtatt cccgcgtggc cgattacatc
      901 gtgaaggtga gcctgtggtc cctgctggtg accctggtgg tgctgtttat catgacagga
      961 accatgctgg gaccagagct gctggcatct atcccagcag ccgtgtatgt gatcgccatc
     1021 ttcatgccac tggcaggata cgcaagcgga tatggcctgg ccaccctgtt tcacctgccc
     1081 cctaactgca agcggaccgt gtgcctggag accggctctc agaatgtgca gctgtgcaca
     1141 gccatcctga agctggcctt cccaccccag tttatcggct ctatgtacat gttcccactg
     1201 ctgtatgccc tgtttcagag cgccgaggcc ggcatcttcg tgctgatcta caagatgtat
     1261 ggctccgaga tgctgcacaa gagagacccc ctggacgagg atgaggacac cgatatctcc
     1321 tacaagaagc tgaaggagga ggagatggcc gatacatctt atggcaccgt gaaggccgag
     1381 aatatcatca tgatggagac agcccagacc agcctgtaag acccagcttt cttgtacaaa
     1441 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1501 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1561 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1621 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1681 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1741 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1801 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1861 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1921 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1981 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2041 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2101 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2161 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2221 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2281 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2341 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2401 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2461 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2521 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2581 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2641 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2701 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2761 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2821 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2881 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2941 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3001 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3061 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3121 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3181 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3241 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3301 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3361 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3421 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3481 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3541 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     3601 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     3661 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3721 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     3781 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     3841 acggccagtc ttaagctcgg gccc
//
