LOCUS       sequence-365870-        2211 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Santa Cruz Garcia AB, Schnur KP, Malik AB, Mo GCH
  TITLE     Gasdermin D pores are dynamically regulated by local 
            phosphoinositide circuitry.
  JOURNAL   Nat Commun. 2022 Jan 10;13(1):52. doi: 10.1038/s41467-021-27692-9.
  PUBMED    35013201
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-01-10"; volume: "13(1):52. doi"; pages: " 
            10.1038/s41467-021-27692-9"
COMMENT     Sequence Label: pcDNA3-PhoDer
FEATURES             Location/Qualifiers
     source          1..2211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             844..1569
                     /codon_start=1
                     /product="photocleavable protein that separates into two 
                     fragments upon exposure to violet light (Zhang et al., 
                     2017)"
                     /label=PhoCl
                     /translation="VIPDYFKQSFPEGYSWERSMTYEDGGICIATNDITMEGDSFINKI
                     HFKGTNFPPNGPVMQKRTVGWEASTEKMYERDGVLKGDVKMKLLLKGGGHYRCDYRTTY
                     KVKQKPVKLPDYHFVDHRIEILSHDKDYNKVKLYEHAVARNSTDSMDELYKGGSGGMVS
                     KGEETITSVIKPDMKNKLRMEGNVNGHAFVIEGEGSGKPFEGIQTIDLEVKEGAPLPFA
                     YDILTTAFHYGNRVFTKYPR"
ORIGIN
        1 aagcttgcgg ccgccaccat ggggtcggcc tttgagcggg tagtccggag agtggtccag
       61 gagctggacc atggtgggga gttcatccct gtgaccagcc tgcagagctc cactggcttc
      121 cagccctact gcctggtggt taggaagccc tcaagctcat ggttctggaa accccgttat
      181 aagtgtgtca acctgtctat caaggacatc ctggagccgg atgccgcgga accagacgtg
      241 cagcgtggca ggagcttcca cttctacgat gccatggatg ggcagataca gggcagcgtg
      301 gagctggcag ccccaggaca ggcaaagatc gcaggcgggg ccgcggtgtc tgacagctcc
      361 agcacctcaa tgaatgtgta ctcgctgagt gtggacccta acacctggca gactctgctc
      421 catgagaggc acctgcggca gccagaacac aaagtcctgc agcagctgcg cagccgcggg
      481 gacaacgtgt acgtggtgac tgaggtgcta cagacacaga aggaggtgga agtcacgcgc
      541 acccacaagc gggagggctc gggccggttt tccctgcccg gagccacgtg cttgcagggt
      601 gagggccagg gccatctgag ccagaagaag acggtcacca tcccctcagg cagcaccctc
      661 gcattccggg tggcccagct ggttattgac tctgacttgg acgtccttct cttcccggat
      721 aagaagcaga ggaccttcca gccacccgcg acaggccaca agcgttccac gagcgaaggc
      781 gcctggccac agctgccctc tggcctctcc atgatgaggt gcctccacaa cggaggtgga
      841 agcgtgatcc ctgactactt caagcagagc ttccccgagg gctacagctg ggagcgcagc
      901 atgacctacg aggacggcgg catctgcatc gccaccaacg acatcacaat ggagggggac
      961 agcttcatca acaagatcca cttcaagggc acgaacttcc cccccaacgg ccccgtgatg
     1021 cagaagagga ccgtgggctg ggaggccagc accgagaaga tgtacgagcg cgacggcgtg
     1081 ctgaagggcg acgtgaagat gaagctgctg ctgaagggcg gcggccacta tcgctgcgac
     1141 taccgcacca cctacaaggt caagcagaag cccgtaaagc tgcccgacta ccacttcgtg
     1201 gaccaccgca tcgagatcct gagccacgac aaggactaca acaaggtgaa gctgtacgag
     1261 cacgccgtgg cccgcaactc caccgacagc atggacgagc tgtacaaggg tggcagcggt
     1321 ggcatggtga gcaagggcga ggagaccatt acaagcgtga tcaagcctga catgaagaac
     1381 aagctgcgca tggagggcaa cgtgaacggc cacgccttcg tgatcgaggg cgagggcagc
     1441 ggcaagccct tcgagggcat ccagacgatt gatttggagg tgaaggaggg cgccccgctg
     1501 cccttcgcct acgacatcct gaccaccgcc ttccactacg gcaaccgcgt gttcaccaag
     1561 tacccacggg gaggtggggt ccctgcggag ggggcgttca ctgaagactt ccagggccta
     1621 cgggcagagg tggagaccat ctccaaggaa ctggagcttt tggacagaga gctgtgccag
     1681 ctgctgctgg agggcctgga gggggtgctg cgggaccagc tggccctgcg agccttggag
     1741 gaggcgctgg agcagggcca gagccttggg ccggtggagc ccctggacgg tccagcaggt
     1801 gctgtcctgg agtgcctggt gttgtcctcc ggaatgctgg tgccggaact cgctatccct
     1861 gttgtctacc tgctgggggc actgaccatg ctgagtgaaa cgcagcacaa gctgctggcg
     1921 gaggcgctgg agtcgcagac cctgttgggg ccgctcgagc tggtgggcag cctcttggag
     1981 cagagtgccc cgtggcagga gcgcagcacc atgtccctgc cccccgggct cctggggaac
     2041 agctggggcg aaggagcacc ggcctgggtc ttgctggacg agtgtggcct agagctgggg
     2101 gaggacactc cccacgtgtg ctgggagccg caggcccagg gccgcatgtg tgcactctac
     2161 gcctccctgg cactgctatc aggactgagc caggagcccc actgagaatt c
//
