LOCUS       sequence-366314-       14914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knockdown of murine KRAS gene by targeting the promoter region via 
            CRISPRi system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14914)
  AUTHORS   Qian F, Nettleford SK, Zhou J, Arner BE, Hall MA, Sharma A, 
            Annageldiyev C, Rossi RM, Tukaramrao DB, Sarkar D, Hegde S, Gandhi 
            UH, Finch ER, Goodfield L, Quickel MD, Claxton DF, Paulson RF, 
            Prabhu KS
  TITLE     Activation of GPR44 decreases severity of myeloid leukemia via 
            specific targeting of leukemia initiating stem cells.
  JOURNAL   Cell Rep. 2023 Jul 25;42(7):112794. doi: 
            10.1016/j.celrep.2023.112794. Epub 2023 Jul 18.
  PUBMED    37459233
REFERENCE   2  (bases 1 to 14914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-07-25"; volume: "42(7):112794. doi"; pages: " 
            10.1016/j.celrep.2023.112794. Epub 2023 Jul 18"
COMMENT     Sequence Label: pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1
FEATURES             Location/Qualifiers
     source          1..14914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..87)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(241..829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(321..340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1000..1791,1792..1860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1623..1642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1861..1965)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2040..2059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2231..2610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2612..2810
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2765..2785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2828..3008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3055..3180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3673..3906
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4091..4135
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4433..4550
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(4594..4627)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(4638..4713)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(4734..4982)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(4792..4811)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(4962..4982)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        5015..6226
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6063..6082
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6255..6264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6264..6329
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6336..6356
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6366..6392,6393..6395,6396..8879,8880..8885,8886..8951,
                     8952..8954,8955..10469)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(6366..6392,6393..6395,6396..8882,8883..8885,
                     8886..10469)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6366..10469
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10482..10502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10536..10730
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             10797..10817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10824..10877
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(10884..10886,10887..11597)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(10884..10886,10887..11597)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(10926..10947)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11187..11206)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11534..11555
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(11613..11646)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    11671..12259
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11724..11744)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12142..12153)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12262..12278)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12263..12279)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             12784..12964
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12990..13007)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12996..13220
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13266..13694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13353..13372)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13563..13584
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13703..13723)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13747..13957
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        13769..13965
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      13816..13951
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13878..13897
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        14013..14060
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             14079..14453
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    14583..14704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14620..14639)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14674..14693
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(14753..14769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14753..14769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14766..14788)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14777..14793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14801..14807,14808..14825,14826..14831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14846..14867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
       61 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      121 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      661 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      961 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1861 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1981 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     2041 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     2101 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     2161 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     2221 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2281 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2341 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2401 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2461 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2521 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2581 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2641 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2701 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2761 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     2821 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2881 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2941 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3001 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     3061 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     3121 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     3181 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3241 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3301 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3361 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3421 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3481 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3541 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3601 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3661 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3721 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3781 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3841 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3901 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3961 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     4021 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     4081 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     4141 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     4201 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4261 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4321 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4381 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     4441 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     4501 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     4561 acagggacag cagagatcca gtttggttaa ttaataactt cgtatagcat acattatacg
     4621 aagttattaa aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
     4681 cttattttaa cttgctattt ctagctctaa aaccagcgct gccgcctcag ccgggtgttt
     4741 cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc
     4801 atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac
     4861 tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt aattccaatt
     4921 atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc
     4981 tcttaattaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt tttggcgcct
     5041 cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag
     5101 cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc
     5161 ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca
     5221 ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct
     5281 gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg
     5341 gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt
     5401 gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc
     5461 cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag
     5521 caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt
     5581 cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat
     5641 ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg
     5701 ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag
     5761 aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac
     5821 ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta
     5881 taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac
     5941 gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg
     6001 ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta
     6061 gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt
     6121 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag
     6181 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag cttgggctgc
     6241 aggtcgactc tagagccacc atggactaca aagaccatga cggtgattat aaagatcatg
     6301 acatcgatta caaggatgac gatgacaaga tggcccccaa gaagaagagg aaggtgggcc
     6361 gcggaatgga caagaagtac tccattgggc tcgccatcgg cacaaacagc gtcggctggg
     6421 ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg
     6481 atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc ggggaaaccg
     6541 ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga
     6601 tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc
     6661 ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct
     6721 ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga
     6781 ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg
     6841 cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca
     6901 gcgatgtcga caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga
     6961 acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat
     7021 cccggcggct cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg
     7081 gtaatcttat cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg
     7141 ccgaagatgc caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc
     7201 tggcccagat cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg
     7261 ccattctgct gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg
     7321 ctagtatgat caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg
     7381 tcagacagca actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct
     7441 acgccggata cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca
     7501 tcttggaaaa aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt
     7561 tgcgcaaaca gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac
     7621 tgcacgctat cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa
     7681 agattgagaa aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa
     7741 attccagatt cgcgtggatg actcgcaaat cagaagagac catcactccc tggaacttcg
     7801 aggaagtcgt ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg
     7861 ataaaaatct gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca
     7921 cagtttataa cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat
     7981 tcctgtctgg agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag
     8041 ttaccgtgaa acagctcaaa gaagactatt tcaaaaagat tgaatgtttc gactctgttg
     8101 aaatcagcgg agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga
     8161 aaatcattaa agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca
     8221 ttgtcctcac ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt
     8281 acgctcatct cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat
     8341 gggggcggct gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa
     8401 tcctggattt tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg
     8461 atgactctct cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca
     8521 gtcttcacga gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc
     8581 agaccgttaa ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata
     8641 tcgttatcga gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg
     8701 aaaggatgaa gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac
     8761 acccagttga aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg
     8821 gcagggacat gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg
     8881 atgccatcgt gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa
     8941 gatccgataa aaatagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa
     9001 tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata
     9061 atctgactaa ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa
     9121 ggcagcttgt tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca
     9181 tgaacaccaa gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga
     9241 agtctaagct ggtctcagat ttcagaaagg actttcagtt ttataaggtg agagagatca
     9301 acaattacca ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca
     9361 aaaaatatcc caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta
     9421 ggaaaatgat cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt
     9481 acagcaatat tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga
     9541 agcgaccact tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg
     9601 atttcgcgac agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg
     9661 aagtacagac cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc
     9721 tgatcgcacg caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag
     9781 tcgcttacag tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa
     9841 gcgtcaagga actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca
     9901 tcgactttct cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc
     9961 ccaagtactc tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg
    10021 agctgcagaa aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg
    10081 ccagccacta tgaaaagctc aaagggtctc ccgaagataa tgagcagaag cagctgttcg
    10141 tggaacaaca caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa
    10201 gagtgatcct cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg
    10261 ataagcccat cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg
    10321 gcgcgcctgc agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta
    10381 caaaggaggt cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa
    10441 gaatcgacct ctctcagctc ggtggagaca gcagggctga ccccaagaag aagaggaagg
    10501 tggctagcga tgctaagtca ctgactgcct ggtcccggac actggtgacc ttcaaggatg
    10561 tgtttgtgga cttcaccagg gaggagtgga agctgctgga cactgctcag cagatcctgt
    10621 acagaaatgt gatgctggag aactataaga acctggtttc cttgggttat cagcttacta
    10681 agccagatgt gatcctccgg ttggagaagg gagaagagcc ctggctggtg gagagagaaa
    10741 ttcaccaaga gacccatcct gattcagaga ctgcatttga aatcaaatca tcagttccga
    10801 aaaagaaacg caaagttgct agcgagggca gaggaagtct tctaacatgc ggtgacgtgg
    10861 aggagaatcc cggccctggt accgtgagca agggcgagga gctgttcacc ggggtggtgc
    10921 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
    10981 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
    11041 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
    11101 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
    11161 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
    11221 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
    11281 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
    11341 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
    11401 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
    11461 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
    11521 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
    11581 tggacgagct gtacaagacc ggttgataat agataacttc gtatagcata cattatacga
    11641 agttatgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
    11701 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
    11761 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
    11821 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
    11881 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
    11941 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    12001 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    12061 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
    12121 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    12181 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    12241 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
    12301 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
    12361 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
    12421 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
    12481 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    12541 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
    12601 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
    12661 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
    12721 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
    12781 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    12841 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    12901 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    12961 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
    13021 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
    13081 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
    13141 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
    13201 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
    13261 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    13321 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    13381 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    13441 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    13501 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    13561 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    13621 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    13681 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
    13741 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
    13801 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    13861 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
    13921 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
    13981 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
    14041 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
    14101 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
    14161 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
    14221 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
    14281 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
    14341 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
    14401 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
    14461 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    14521 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    14581 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    14641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    14701 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    14761 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    14821 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    14881 cactgcccgc tttccagtcg ggaaacctgt cgtg
//
