LOCUS       sequence-366348-        5125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-AB1
FEATURES             Location/Qualifiers
     source          1..5125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1177..1311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1201..1220)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1255..1274
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1424..1444)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1424..1442)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1463..1479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1463..1479)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1476..1498)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1487..1503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1511..1517,1518..1535,1536..1541))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1556..1577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1694..1711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1865..2453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1945..1964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2624..3415,3416..3484))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3247..3266
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3485..3589)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3615..4070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3752..3773)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3964..3983
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4141..5125
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctatga gctttagcca
      121 ggagtctggt gcccatcatc acccccagga ttatgcagga ctttctgatg aggaggatga
      181 agatgatccc tgcagcctaa agtcacactt ttacctacag cccacacatt cagtgatggg
      241 ggacagtgag atgctgagtc cttccaagct cagctgcact gaaagtgaga gtgattcctc
      301 tggagagagt gaaggaggta cttctaaaga ctcctcaacc accccaactg gtagcaaagc
      361 caaaaggact ctggtaaagc ctccttactc ttacattgct ctaatcacca tggctatcct
      421 gcagagtccc cacaagaaac tgactctcag tggcatctgt gattttatca gcagcaagtt
      481 cccttactac aaggacaagt tccctgcttg gcagaattcc atcaggcaca acctgtccct
      541 taatgactgc tttattaaaa taccacggga accaggaaat ccgggaaaag gcaattattg
      601 gacactggac cctgcttctg aggacatgtt tgataacgga agcttcctta gaaggaggaa
      661 aaggtttaag aggcaccaac aggagttctt taaggatgga ctcatgatgt acaactctct
      721 gccttactac agaccctaca gtgctatcca acctcagcca gtgctccagc agacttcact
      781 gacatgcatg gcaataccag agaccttgcc catgtcaact caccttgcac cctacccaga
      841 cataaagagg aaggtttctt accctgccca gggggtacac aggggtttta aagcccagga
      901 tgctgataac catcccaata attcacagag caaatgttca ttcagtattg agaacatcat
      961 gaggaaaccc aaggagccag agccaaatat tcaaagcttc aactcacact ggaactataa
     1021 tcatgtattc cagaggccaa gttcctgcct tctcccagct gtgcttaact tatccactgg
     1081 ccctctgcta gccaatactc agggtgccag gcaatacaac ctcatacaat tccctgggtg
     1141 ttactgatct agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt
     1201 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     1261 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1321 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaattcgcg
     1381 gccgcggcgc caatgcattg ggcccggtac ccagcttttg ttccctttag tgagggttaa
     1441 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     1501 caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt gcctaatgag
     1561 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1621 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1681 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1741 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1801 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1861 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1921 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1981 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2041 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2101 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2161 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2221 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2281 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2341 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2401 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2461 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2521 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2581 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2641 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2701 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2761 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2821 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2881 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2941 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3001 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3061 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3121 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3181 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3241 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3301 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3361 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3421 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3481 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3541 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3601 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     3661 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3721 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3781 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3841 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     3901 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     3961 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4021 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     4081 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     4141 gtcgaccgcc aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg
     4201 gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg
     4261 gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacctg
     4321 gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt
     4381 ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg
     4441 gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta
     4501 ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat atatgggttt
     4561 tcctattgac gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc
     4621 ctaataccgc ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc
     4681 tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat
     4741 tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg
     4801 acgtcaatgg gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca
     4861 atgacggtaa atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt
     4921 acattactat tggaagtacg ccagggtaca ttggcagtac tcccattgac gtcaatggcg
     4981 gtaaatggcc cgcgatggct gccaagtaca tccccattga cgtcaatggg gaggggcaat
     5041 gacgcaaatg ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat gggaggtcta
     5101 tataagcaat gctcgtttag ggaac
//
