LOCUS       sequence-366356-        5149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-AB2
FEATURES             Location/Qualifiers
     source          1..5149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1201..1335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1225..1244)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1279..1298
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1448..1468)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1448..1466)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1487..1503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1487..1503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1500..1522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1511..1527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1535..1541,1542..1559,1560..1565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1580..1601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1718..1735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1969..1988)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2648..3439,3440..3508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3271..3290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3509..3613)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3639..4094)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3776..3797)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3988..4007
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4165..5149
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctatga gctttagcca
      121 ggagtctggt gcccatcatc acccccagga ttatgcagga ctttctgatg aggaggatga
      181 agcagctgcg gccgctgcag gagaagatga tccctgcagc ctaaagtcac acttttacct
      241 acagcccaca cattcagtga tgggggacag tgagatgctg agtccttcca agctcagctg
      301 cactgaaagt gagagtgatt cctctggaga gagtgaagga ggtacttcta aagactcctc
      361 aaccacccca actggtagca aagccaaaag gactctggta aagcctcctt actcttacat
      421 tgctctaatc accatggcta tcctgcagag tccccacaag aaactgactc tcagtggcat
      481 ctgtgatttt atcagcagca agttccctta ctacaaggac aagttccctg cttggcagaa
      541 ttccatcagg cacaacctgt cccttaatga ctgctttatt aaaataccac gggaaccagg
      601 aaatccggga aaaggcaatt attggacact ggaccctgct tctgaggaca tgtttgataa
      661 cggaagcttc cttagaagga ggaaaaggtt taagaggcac caacaggagt tctttaagga
      721 tggactcatg atgtacaact ctctgcctta ctacagaccc tacagtgcta tccaacctca
      781 gccagtgctc cagcagactt cactgacatg catggcaata ccagagacct tgcccatgtc
      841 aactcacctt gcaccctacc cagacataaa gaggaaggtt tcttaccctg cccagggggt
      901 acacaggggt tttaaagccc aggatgctga taaccatccc aataattcac agagcaaatg
      961 ttcattcagt attgagaaca tcatgaggaa acccaaggag ccagagccaa atattcaaag
     1021 cttcaactca cactggaact ataatcatgt attccagagg ccaagttcct gccttctccc
     1081 agctgtgctt aacttatcca ctggccctct gctagccaat actcagggtg ccaggcaata
     1141 caacctcata caattccctg ggtgttactg atctagaact atagtgagtc gtattacgta
     1201 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     1261 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     1321 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     1381 gtgtgggagg ttttttaatt cgcggccgcg gcgccaatgc attgggcccg gtacccagct
     1441 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     1501 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccggg agcataaagt
     1561 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1621 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1681 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1741 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1801 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1921 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3601 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     3661 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3721 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3781 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3841 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3901 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3961 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     4021 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     4081 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     4141 gcgggcctct tcgctattac gccagtcgac cgccaattca atatggcgta tatggactca
     4201 tgccaattca atatggtgga tctggacctg tgccaattca atatggcgta tatggactcg
     4261 tgccaattca atatggtgga tctggacccc agccaattca atatggcgga cttggcacca
     4321 tgccaattca atatggcgga cctggcactg tgccaactgg ggaggggtct acttggcacg
     4381 gtgccaagtt tgaggagggg tcttggccct gtgccaagtc cgccatattg aattggcatg
     4441 gtgccaataa tggcggccat attggctata tgccaggatc aatatatagg caatatccaa
     4501 tatggcccta tgccaatatg gctattggcc aggttcaata ctatgtattg gccctatgcc
     4561 atatagtatt ccatatatgg gttttcctat tgacgtagat agcccctccc aatgggcggt
     4621 cccatatacc atatatgggg cttcctaata ccgcccatag ccactccccc attgacgtca
     4681 atggtctcta tatatggtct ttcctattga cgtcatatgg gcggtcctat tgacgtatat
     4741 ggcgcctccc ccattgacgt caattacggt aaatggcccg cctggctcaa tgcccattga
     4801 cgtcaatagg accacccacc attgacgtca atgggatggc tcattgccca ttcatatccg
     4861 ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc cacttggcag tacatcaata
     4921 tctattaata gtaacttggc aagtacatta ctattggaag tacgccaggg tacattggca
     4981 gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat ggctgccaag tacatcccca
     5041 ttgacgtcaa tggggagggg caatgacgca aatgggcgtt ccattgacgt aaatgggcgg
     5101 taggcgtgcc taatgggagg tctatataag caatgctcgt ttagggaac
//
