LOCUS       sequence-36638-e        9620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Petersen LK, Stowers RS
  TITLE     A Gateway MultiSite Recombination Cloning Toolkit.
  JOURNAL   PLoS One. 2011;6(9):e24531. Epub 2011 Sep 9.
  PUBMED    21931740
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(9)"; pages: "e24531. Epub 2011 Sep 9"
COMMENT     Sequence Label: pDESThaw
FEATURES             Location/Qualifiers
     source          1..9620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2354..2423
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    2703..2827
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2852..2857,2858..2875,2876..2882)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2936..3616
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(3002..3021)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3936..4241
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4120..4139
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4282..4406)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4774..4792)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(8975..8994)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8976..8994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9004..9021)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9004..9020)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9013..9035)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9161..9617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9299..9320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9511..9530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggccactgtt taaacttggc
     2221 gcgcccgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2281 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtta ccgtcgacga
     2341 tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg
     2401 gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt
     2461 tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg
     2521 tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg
     2581 tcagcgggtt ctcgacggtc acggcgggca tgtcgacaag ccgaacatat ggctagctat
     2641 acgcgttata ccggtatcct gcaggtagca tgcaagagat cttataggcc tcgagttatc
     2701 aaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
     2761 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
     2821 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
     2881 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga
     2941 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     3001 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     3061 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     3121 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     3181 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     3241 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     3301 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     3361 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     3421 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     3481 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     3541 cagaatgctt aatgaattac aacagtactg cgatgagtgg caggcggggc gtaatctaga
     3601 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
     3661 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc
     3721 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
     3781 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
     3841 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
     3901 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
     3961 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg
     4021 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtcccccgtg
     4081 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     4141 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     4201 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac
     4261 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta
     4321 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     4381 cgttcagctt tcttgtacaa agtggttcga taacgttcga ggtcgactct agataaagcc
     4441 aaatagaaaa ttattcagtt cctggcttaa gtttttaaaa gtgatattat ttatttggtt
     4501 gtaaccaacc aaaagaatgt aaataactaa tacataatta tgttagtttt aagttagcaa
     4561 caaattgatt ttagctatat tagctacttg gttaataaat agaatatatt tatttaaaga
     4621 taattgcgtt tttattgtca gggagtgagt ttgcttaaaa actcgtttag atccaggatc
     4681 cagatccact agtggcctat gcggccgcgg atctgatccc gggcgggtac ctttgaattc
     4741 cacttaatta aggccggccc aattctagta tgtatgtaag ttaataaaac ccatttttgc
     4801 ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat tgaacttatc
     4861 tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt ctttcgatta
     4921 cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct tctctgtcca
     4981 cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag
     5041 cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc gtgacgaagc
     5101 tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc ctctcgcttc
     5161 ttatttttgt ttgttttttg agtgattggg gtggtgattg gtttttgggt gggtaagcag
     5221 gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga
     5281 ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc
     5341 ttaagttcac ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga
     5401 actgcgaccc acaaaaatcc caaaccgcaa tcgcacaaac aaatagtgac acgaaacaga
     5461 ttattctggt agctgtgctc gctatataag acaattttta agatcatatc atgatcaaga
     5521 catctaaagg cattcatttt cgactacatt cttttttaca aaaaatataa caaccagata
     5581 ttttaagctg atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat
     5641 acttcgtttt gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct
     5701 atcattgcag ggtgacagcg gacgcttcgc agagctgcat taaccagggc ttcgggcagg
     5761 ccaaaaacta cggcacgctc ctgccaccca gtccgccgga ggactccggt tcagggagcg
     5821 gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt ggggcggtca
     5881 atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta ttctgcaacg
     5941 agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt ctattcgcag
     6001 tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa actcagtttg
     6061 cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg ccggaaagac
     6121 gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat cgccatccgg
     6181 gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca ggtgcgccta
     6241 tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc tgattttcca
     6301 ggccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc gcgtggatca
     6361 ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg tgcccggcag
     6421 ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg aggcactaac
     6481 cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcat ttaccgccca
     6541 cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca tcctgaccat
     6601 tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga tggccgaggg
     6661 cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct agtgagttcg
     6721 atgtgtttat taagggtatc tagcattaca ttacatctca actcctatcc agcgtgggtg
     6781 cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg gccgttgtgc
     6841 ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atggcgacaa ttttgctatt
     6901 agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatttgga gaagccactg
     6961 gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt ccgggcggtc
     7021 ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt
     7081 cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa ttcttggaaa
     7141 caaattcgct agattttagt tagaattgcc tgattccaca cccttcttag tttttttcaa
     7201 tgagatgtat agtttatagt tttgcagaaa ataaataaat ttcatttaac tcgcgaacat
     7261 gttgaagata tgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat
     7321 cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggcgtga tgaatatcaa
     7381 cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa
     7441 tgtaagtctt gtttagaata catttgcata ttaataattt actaactttc taatgaatcg
     7501 attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac
     7561 tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattaccg ctttttctca
     7621 cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc
     7681 tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg
     7741 gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg
     7801 ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat
     7861 acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta
     7921 accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc
     7981 ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg
     8041 actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa
     8101 gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt
     8161 ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt
     8221 atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc cccttcgaac
     8281 atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca cctgcatacc
     8341 ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt tatatacata
     8401 cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt tcgtagttgc
     8461 tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg acgaccttgg
     8521 agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttccgag tggttcattt
     8581 cgtctcaata gaaattagta ataaatattt gtatgtacaa tttatttgct ccaatatatt
     8641 tgtatatatt tccctcacag ctatatttat tctaatttaa tattatgact ttttaaggta
     8701 attttttgtg acctgttcgg agtgattagc gttacaattt gaactgaaag tgacatccag
     8761 tgtttgttcc ttgtgtagat gcaggtagca tgcaagagat cttataggcc tcgagttaac
     8821 gttcgaggtc gactctagat aaagctttag agctcttctt aagaaggatc cagatccact
     8881 agtggcctat gcggccgcgg atctgatccc gggcgggtac ctttgaattc cacttaatta
     8941 agaaggccgg cccttcctca gcgtggccaa ttcgccctat agtgagtcgt attacgcgcg
     9001 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     9061 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     9121 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt
     9181 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat ttttttaacc aataggccga
     9241 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     9301 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     9361 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     9421 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     9481 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     9541 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     9601 gccgctacag ggcgcgtcag
//
